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The map label for this gene is vspIM [H]

Identifier: 108562880

GI number: 108562880

Start: 467482

End: 469119

Strand: Direct

Name: vspIM [H]

Synonym: HPAG1_0455

Alternate gene names: 108562880

Gene position: 467482-469119 (Clockwise)

Preceding gene: 108562879

Following gene: 108562881

Centisome position: 29.28

GC content: 33.46

Gene sequence:

>1638_bases
ATGCCTTCAAACGCTCTTTTGATTGAAGAAATCGCTCGCTTAATCAATGTTTCTCATAGCAGCGTGCATAACTGGATCAA
AACCAATCTTTTAGAGAAACTAGAGATTGATCATAAAATTTATGTGAAAACGAGTTCTTTTTTAGATTTTTGCCGCAACC
ATTTAGGGAAAAACAAGCTCAACAAATACGCTAACAAATCCTTAAAAGGCGCGCATAACCATCAAGAATTGATTTTAAAA
TACCTAAAAATATTAGAAAATAGCTCTGATTTAGAAAAGTTGGGTTCTCATTATGAAAAAGAGCTTTCTAATACCACCAG
AAATTTAGAAGGCATTTACTACACTCCTAACAGGATAGTAGAACAACGTTTCACCCTCCCTAAAGATTTTGATGCCTCTC
AAGCGATTTTTTGCGATCCGGCTGTGGGGAGCGGGAATTTTATCATGCATGCTTTAAAACTGGGTTTTAAGGTTGAAAAT
ATTTATGGCTATGATACGGACGCTTTCGCTATCGCTTTGACTAAAAAGCGTATTAAAGAGCGTTATCATTTAGATTGCCC
TAATATTGCACAAAAAGATTTTTTAAGTTTAAAACACACCCCACAATTTGATTGCATTTTCACTAACCCACCATGGGGCA
AGAAATACAATCAAGACCAAAAAGAAAATTTCAAACAGCAATTCAACCTCTCTCAAAGCCTAGATAGCGCGTCGCTCTTT
TTTATAGCGAGTTTGAATTGTTTAAAAGAAAACGCTCATTTGGGGCTATTATTACCCGAAAGTTGTTTGAATATTGATGC
GTTTAGCAAAATGCGAGAAATGGCTCTGAAGTTTCAAATTAAAAGCCTGATTGATTTCAACAAACCCTTTAAAACCCTAA
TGACTAAGGCTGTGGGTTTGGTGCTTAAAAAAACCCCTAACAAGGATCAAAAAATCTCATGCTTTTATCAAAATAGCAAG
TTCAAACGCTCGCCCTCTTCTTTTTTTAACAACCCTAAAAAGATTTTTAATATCCATTGCTCTAATAAAGAAAATAAAAT
TTTAGACCACCTTTTTTCCCTTCCTCATATCACTTTAAAAAATAACGCTCATTTTGCTTTAGGGATTGTTACAGGCAACA
ATAAAGAAAAATTACATTCCAAGCAAGAAAAAAATACCATTCCCATTTTTAGGGGTTCAGATATTTTAAAAGACAGATTA
AAAGCCCCTAGCCAATTCATTAACGCTGATTTAAAAGACTGCCAACAAGTCGCCCCATTAAGCCTTTATCAGGCTAGAGA
AAAAATCGTGTATAAATTCATTTCTTCAAAGCTTGTCTTTTTTTGTGATAATGAGCAACGCCTTTTTTTAAATAGCGCGA
ACATGTTTGTTTTAAAAGAAAATTTCCCTATCAACGCTAATGCGCTAAAAGAATTATTAAACAGCGATTTAATGCAATTT
ATTTTTGAATCGCTTTTTAAAACGCATAAAATCTTAAGAAAAGATTTGGAATGTTTGCCCCTATTTGCGCAATTTATCAA
CAATAGTTTTGATGAAAAATTTTATTTAAAAAATTTGGGGATAGAAAAAAAAGATCCTAAACATTTTACAATCAGGAAAA
ATCATGCACATCGCTTGTCTTTTGGCTTTAGGGGATAA

Upstream 100 bases:

>100_bases
CCTTTTATGAAACGCATGGCAAAGGGCTAAACACTTCCCTCTTTTTCAAACGCCTTGTGGCGTTTAATGTGAGTTATGTT
TATAGTTTTTAGGGGGGTAA

Downstream 100 bases:

>100_bases
CCTCATCACGCTTAGCCTTTTAAAAGAAATCGCTTCCAAACAGCAACAACCCCTTAAAATCCTAGGCACTCATTTGACTT
TAAAAATCGCCAGGCTTTTA

Product: type II adenine specific methyltransferase

Products: NA

Alternate protein names: M.VspI; Adenine-specific methyltransferase VspI [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MPSNALLIEEIARLINVSHSSVHNWIKTNLLEKLEIDHKIYVKTSSFLDFCRNHLGKNKLNKYANKSLKGAHNHQELILK
YLKILENSSDLEKLGSHYEKELSNTTRNLEGIYYTPNRIVEQRFTLPKDFDASQAIFCDPAVGSGNFIMHALKLGFKVEN
IYGYDTDAFAIALTKKRIKERYHLDCPNIAQKDFLSLKHTPQFDCIFTNPPWGKKYNQDQKENFKQQFNLSQSLDSASLF
FIASLNCLKENAHLGLLLPESCLNIDAFSKMREMALKFQIKSLIDFNKPFKTLMTKAVGLVLKKTPNKDQKISCFYQNSK
FKRSPSSFFNNPKKIFNIHCSNKENKILDHLFSLPHITLKNNAHFALGIVTGNNKEKLHSKQEKNTIPIFRGSDILKDRL
KAPSQFINADLKDCQQVAPLSLYQAREKIVYKFISSKLVFFCDNEQRLFLNSANMFVLKENFPINANALKELLNSDLMQF
IFESLFKTHKILRKDLECLPLFAQFINNSFDEKFYLKNLGIEKKDPKHFTIRKNHAHRLSFGFRG

Sequences:

>Translated_545_residues
MPSNALLIEEIARLINVSHSSVHNWIKTNLLEKLEIDHKIYVKTSSFLDFCRNHLGKNKLNKYANKSLKGAHNHQELILK
YLKILENSSDLEKLGSHYEKELSNTTRNLEGIYYTPNRIVEQRFTLPKDFDASQAIFCDPAVGSGNFIMHALKLGFKVEN
IYGYDTDAFAIALTKKRIKERYHLDCPNIAQKDFLSLKHTPQFDCIFTNPPWGKKYNQDQKENFKQQFNLSQSLDSASLF
FIASLNCLKENAHLGLLLPESCLNIDAFSKMREMALKFQIKSLIDFNKPFKTLMTKAVGLVLKKTPNKDQKISCFYQNSK
FKRSPSSFFNNPKKIFNIHCSNKENKILDHLFSLPHITLKNNAHFALGIVTGNNKEKLHSKQEKNTIPIFRGSDILKDRL
KAPSQFINADLKDCQQVAPLSLYQAREKIVYKFISSKLVFFCDNEQRLFLNSANMFVLKENFPINANALKELLNSDLMQF
IFESLFKTHKILRKDLECLPLFAQFINNSFDEKFYLKNLGIEKKDPKHFTIRKNHAHRLSFGFRG
>Mature_544_residues
PSNALLIEEIARLINVSHSSVHNWIKTNLLEKLEIDHKIYVKTSSFLDFCRNHLGKNKLNKYANKSLKGAHNHQELILKY
LKILENSSDLEKLGSHYEKELSNTTRNLEGIYYTPNRIVEQRFTLPKDFDASQAIFCDPAVGSGNFIMHALKLGFKVENI
YGYDTDAFAIALTKKRIKERYHLDCPNIAQKDFLSLKHTPQFDCIFTNPPWGKKYNQDQKENFKQQFNLSQSLDSASLFF
IASLNCLKENAHLGLLLPESCLNIDAFSKMREMALKFQIKSLIDFNKPFKTLMTKAVGLVLKKTPNKDQKISCFYQNSKF
KRSPSSFFNNPKKIFNIHCSNKENKILDHLFSLPHITLKNNAHFALGIVTGNNKEKLHSKQEKNTIPIFRGSDILKDRLK
APSQFINADLKDCQQVAPLSLYQAREKIVYKFISSKLVFFCDNEQRLFLNSANMFVLKENFPINANALKELLNSDLMQFI
FESLFKTHKILRKDLECLPLFAQFINNSFDEKFYLKNLGIEKKDPKHFTIRKNHAHRLSFGFRG

Specific function: This methylase recognizes the double-stranded sequence ATTAAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the VspI endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 63141; Mature: 63010

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNALLIEEIARLINVSHSSVHNWIKTNLLEKLEIDHKIYVKTSSFLDFCRNHLGKNKL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHH
NKYANKSLKGAHNHQELILKYLKILENSSDLEKLGSHYEKELSNTTRNLEGIYYTPNRIV
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHH
EQRFTLPKDFDASQAIFCDPAVGSGNFIMHALKLGFKVENIYGYDTDAFAIALTKKRIKE
HHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCHHEEHHHHHHHHH
RYHLDCPNIAQKDFLSLKHTPQFDCIFTNPPWGKKYNQDQKENFKQQFNLSQSLDSASLF
HHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCHHHCCCCHHHH
FIASLNCLKENAHLGLLLPESCLNIDAFSKMREMALKFQIKSLIDFNKPFKTLMTKAVGL
HHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VLKKTPNKDQKISCFYQNSKFKRSPSSFFNNPKKIFNIHCSNKENKILDHLFSLPHITLK
HHCCCCCCCCEEEEEEECCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEE
NNAHFALGIVTGNNKEKLHSKQEKNTIPIFRGSDILKDRLKAPSQFINADLKDCQQVAPL
CCCEEEEEEEECCCHHHHHHHHHCCCCCEECCCHHHHHHHCCCHHHHCCCHHHHHHHCCH
SLYQAREKIVYKFISSKLVFFCDNEQRLFLNSANMFVLKENFPINANALKELLNSDLMQF
HHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
IFESLFKTHKILRKDLECLPLFAQFINNSFDEKFYLKNLGIEKKDPKHFTIRKNHAHRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCEEEC
FGFRG
CCCCC
>Mature Secondary Structure 
PSNALLIEEIARLINVSHSSVHNWIKTNLLEKLEIDHKIYVKTSSFLDFCRNHLGKNKL
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCHHHH
NKYANKSLKGAHNHQELILKYLKILENSSDLEKLGSHYEKELSNTTRNLEGIYYTPNRIV
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHH
EQRFTLPKDFDASQAIFCDPAVGSGNFIMHALKLGFKVENIYGYDTDAFAIALTKKRIKE
HHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCHHEEHHHHHHHHH
RYHLDCPNIAQKDFLSLKHTPQFDCIFTNPPWGKKYNQDQKENFKQQFNLSQSLDSASLF
HHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCHHHCCCCHHHH
FIASLNCLKENAHLGLLLPESCLNIDAFSKMREMALKFQIKSLIDFNKPFKTLMTKAVGL
HHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VLKKTPNKDQKISCFYQNSKFKRSPSSFFNNPKKIFNIHCSNKENKILDHLFSLPHITLK
HHCCCCCCCCEEEEEEECCCCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCEEEE
NNAHFALGIVTGNNKEKLHSKQEKNTIPIFRGSDILKDRLKAPSQFINADLKDCQQVAPL
CCCEEEEEEEECCCHHHHHHHHHCCCCCEECCCHHHHHHHCCCHHHHCCCHHHHHHHCCH
SLYQAREKIVYKFISSKLVFFCDNEQRLFLNSANMFVLKENFPINANALKELLNSDLMQF
HHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHH
IFESLFKTHKILRKDLECLPLFAQFINNSFDEKFYLKNLGIEKKDPKHFTIRKNHAHRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECCCCEEEC
FGFRG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8493116; 7607528 [H]