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The map label for this gene is tuf

Identifier: 103488297

GI number: 103488297

Start: 2973661

End: 2974854

Strand: Reverse

Name: tuf

Synonym: Sala_2820

Alternate gene names: 103488297

Gene position: 2974854-2973661 (Counterclockwise)

Preceding gene: 103488298

Following gene: 103488296

Centisome position: 88.93

GC content: 62.81

Gene sequence:

>1194_bases
ATGGCCAAGGCTAAATTTGAGCGGACGAAGCCGCACTGCAACATCGGCACCATCGGTCACGTCGACCATGGCAAGACGTC
GCTGACCGCCGCGATCACCAAGGTGCTCGCCGAAAACGTCGCGGGTAACGCCGCCGTCGACTTCGCGAACATCGACAAGG
CGCCCGAAGAGCGTGAGCGCGGCATCACCATTTCGACCGCGCACGTCGAATATGAAACCGAAAGCCGCCACTATGCGCAC
GTCGACTGTCCGGGCCACGCCGACTATGTGAAGAACATGATCACCGGCGCCGCGCAGATGGACGGCGCGATCCTCGTTGT
GTCGGCCGCCGACGGCCCGATGCCGCAGACGAAGGAGCACATCCTGCTCGCGAAGCAGGTCGGCGTTCCGACGATGGTTG
TCTTCCTGAACAAGGTCGACCAGCTCGACGATCCCGAACTGCTCGAACTCGTCGAACTCGAAATCCGCGAAGAACTTTCG
AAGCGCGACTTCGATGGCGACAATATCCCGATCATCGCCGGTTCGGCGCTCGCGGCGCTCGAAGGCCGCGACGACAATAT
CGGCAAGGACGCGATCCTGAAGCTGATGGCGGCCGTTGACGAATGGATTCCGCAGCCGGAACGTCCGCTCGACAAGCCCT
TCCTGATGCCGATCGAAGACGTGTTCTCGATCTCGGGTCGCGGCACCGTCGTCACCGGCCGCGTCGAAACCGGCGTTGTC
AAGGTGGGCGAAGAAGTCGAGATCGTCGGCATCAAGGACACCAAGAAGACCGTCGTCACCGGCGTCGAAATGTTCCGCAA
GCTGCTCGACCAGGGCCAGGCCGGCGACAACATCGGCGCGCTGATCCGCGGCGTCGGCCGTGAAGAAGTCGAGCGTGGCC
AGGTTCTGGCGAAGCCTGGCTCGATCACGCCGCACACCGAGTTCACCTCGGAAGTCTATGTCCTGTCGAAGGACGAAGGC
GGTCGTCACACGCCGTTCTTTGCGAACTACCGTCCGCAATTCTACTTCCGCACCACCGACGTCACCGGCGAGGTCATCCT
CCCCGAGGGCACCGAGATGGTCATGCCGGGCGACAACGTCCAGCTGTCGGTCAAGCTGATTGCCCCGATCGCCATGGACC
CGGGTCTGCGCTTCGCCATTCGCGAAGGCGGCCGCACGGTCGGTGCAGGGGTTGTGGCGACCGTCACAAAGTAA

Upstream 100 bases:

>100_bases
CTTGCACCGCGCGGCAAGACCTACTAAGGGCGGCGCCTGATTCAGCGGGCTCGTCCAGGACTGATCAACGCAAACACATC
GAACAAGAAGGTTCAATATC

Downstream 100 bases:

>100_bases
TATAGGGCCACGAGCCGCGCGATTCGGATCGATTCGCGCGGCTCGTAGCTTAAAGGTTTTCGATGGCCGATCCGACTTCC
AGCGCGGAAGTCGGAGCCGG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAENVAGNAAVDFANIDKAPEERERGITISTAHVEYETESRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTKEHILLAKQVGVPTMVVFLNKVDQLDDPELLELVELEIREELS
KRDFDGDNIPIIAGSALAALEGRDDNIGKDAILKLMAAVDEWIPQPERPLDKPFLMPIEDVFSISGRGTVVTGRVETGVV
KVGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAGDNIGALIRGVGREEVERGQVLAKPGSITPHTEFTSEVYVLSKDEG
GRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVQLSVKLIAPIAMDPGLRFAIREGGRTVGAGVVATVTK

Sequences:

>Translated_397_residues
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAENVAGNAAVDFANIDKAPEERERGITISTAHVEYETESRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTKEHILLAKQVGVPTMVVFLNKVDQLDDPELLELVELEIREELS
KRDFDGDNIPIIAGSALAALEGRDDNIGKDAILKLMAAVDEWIPQPERPLDKPFLMPIEDVFSISGRGTVVTGRVETGVV
KVGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAGDNIGALIRGVGREEVERGQVLAKPGSITPHTEFTSEVYVLSKDEG
GRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVQLSVKLIAPIAMDPGLRFAIREGGRTVGAGVVATVTK
>Mature_396_residues
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAENVAGNAAVDFANIDKAPEERERGITISTAHVEYETESRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTKEHILLAKQVGVPTMVVFLNKVDQLDDPELLELVELEIREELSK
RDFDGDNIPIIAGSALAALEGRDDNIGKDAILKLMAAVDEWIPQPERPLDKPFLMPIEDVFSISGRGTVVTGRVETGVVK
VGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAGDNIGALIRGVGREEVERGQVLAKPGSITPHTEFTSEVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVQLSVKLIAPIAMDPGLRFAIREGGRTVGAGVVATVTK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=56.0102301790281, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=30.3769401330377, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=31.2638580931264, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=28.4738041002278, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=28.4738041002278, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=29.5384615384615, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=325, Percent_Identity=30.4615384615385, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI46094014, Length=337, Percent_Identity=30.2670623145401, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194097354, Length=325, Percent_Identity=30.4615384615385, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI194018522, Length=325, Percent_Identity=30.4615384615385, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=29.7397769516729, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI157426893, Length=240, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=31.1594202898551, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.4303797468354, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.4303797468354, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=31.3609467455621, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=31.9502074688797, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=288, Percent_Identity=28.125, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789738, Length=146, Percent_Identity=31.5068493150685, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=132, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.1278195488722, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=31.2638580931264, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=31.2638580931264, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=30.1401869158879, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=428, Percent_Identity=27.1028037383178, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=428, Percent_Identity=27.3364485981308, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=354, Percent_Identity=28.8135593220339, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=29.6703296703297, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=267, Percent_Identity=27.3408239700375, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=26.4957264957265, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556745, Length=135, Percent_Identity=34.0740740740741, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=65.4911838790932, Blast_Score=531, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.3063063063063, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.3063063063063, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=26.4957264957265, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=357, Percent_Identity=27.7310924369748, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=29.2592592592593, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=29.7520661157025, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=142, Percent_Identity=35.2112676056338, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=136, Percent_Identity=30.1470588235294, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=58.6734693877551, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=58.6734693877551, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=48.3116883116883, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=31.4855875831486, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=31.7777777777778, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=31.7777777777778, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.9078947368421, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI17137380, Length=322, Percent_Identity=31.055900621118, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550900, Length=336, Percent_Identity=27.0833333333333, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=27.7580071174377, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=27.7580071174377, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=36.241610738255, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_SPHAL (Q1GP97)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617858.1
- ProteinModelPortal:   Q1GP97
- SMR:   Q1GP97
- GeneID:   4080401
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2820
- NMPDR:   fig|317655.9.peg.2685
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- BioCyc:   SALA317655:SALA_2820-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 42954; Mature: 42822

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAENVAGNAAVDFANIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHC
GITISTAHVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTKEH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLAKQVGVPTMVVFLNKVDQLDDPELLELVELEIREELSKRDFDGDNIPIIAGSALAAL
EEEEHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEE
EGRDDNIGKDAILKLMAAVDEWIPQPERPLDKPFLMPIEDVFSISGRGTVVTGRVETGVV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCEEECEE
KVGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAGDNIGALIRGVGREEVERGQVLAKPG
EECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCC
SITPHTEFTSEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNV
CCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCC
QLSVKLIAPIAMDPGLRFAIREGGRTVGAGVVATVTK
EEEEEEEEEEECCCCCEEEEECCCCEECCCEEEEECC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHVDHGKTSLTAAITKVLAENVAGNAAVDFANIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHC
GITISTAHVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTKEH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLAKQVGVPTMVVFLNKVDQLDDPELLELVELEIREELSKRDFDGDNIPIIAGSALAAL
EEEEHHCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEE
EGRDDNIGKDAILKLMAAVDEWIPQPERPLDKPFLMPIEDVFSISGRGTVVTGRVETGVV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCEEECEE
KVGEEVEIVGIKDTKKTVVTGVEMFRKLLDQGQAGDNIGALIRGVGREEVERGQVLAKPG
EECCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCC
SITPHTEFTSEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNV
CCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCC
QLSVKLIAPIAMDPGLRFAIREGGRTVGAGVVATVTK
EEEEEEEEEEECCCCCEEEEECCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA