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The map label for this gene is gdhA [H]

Identifier: 103488248

GI number: 103488248

Start: 2925064

End: 2926419

Strand: Reverse

Name: gdhA [H]

Synonym: Sala_2771

Alternate gene names: 103488248

Gene position: 2926419-2925064 (Counterclockwise)

Preceding gene: 103488250

Following gene: 103488247

Centisome position: 87.48

GC content: 63.27

Gene sequence:

>1356_bases
ATGGCAGTTTCGGATCACGTCGATTTTGCGACGTTCATGGAGGGCGTGAAACGGCGCAACCCGGGGCAGCCCGAGTTCGT
CCAGGCGGTGCAGGAAGTGTCCGAGGACATTTTCGATTTCATCGCCGACAAGGAAGAATATCACGCGCAGCAGATACTGC
GCCGCATCGCCGAACCCGACCGCGTCGTGTCCTTCCGCGTCTGCTGGGAGGACGACAATGGCAATATCCGCGTCCAGCGC
GGCTGGCGCGTCCAGAACAACAACGCCATCGGTCCGTACAAGGGCGGCATCCGCTTTCACCCCAGCGTCACCGAGAGCGT
GCTCAAGTTCCTCGCGTTCGAGCAGACGTTCAAGAATGCGCTGACCGGGCTGCCGATGGGCGGCGGCAAGGGCGGGTCGA
ACTTCAACCCCAAGGGCAAGAGCGTGCGCGAGATCATGCGCTTTTGCCAGAGCTTCATGACCGAGCTGTACCGCCACATC
GGCGCCGACGTCGACGTGCCCGCGGGCGACATCGGGGTCGGCGGACGCGAGATCGGCTTCATGTTCGGCCAGTATAAACG
CATCACCAACGAGTTTACCGGGGTGCTCACCGGCAAGGGGCTCGAATGGGGCGGCTCGCTGATCCGCACCGAGGCGACGG
GTTACGGCGCGGTTTATTTCCTCGCCAACATGCTCGCGGCGAAGGGGCAGGACCTCGTCGGCAAGAGCGCGGTCATTTCG
GGTTCGGGCAATGTCGCGACACACGCCGCCGAAAAGATCGTCCAGCTTGGCGGCAAGGTACTGACGCTGTCCGATTCGGG
CGGCTTCATCCACGATCCCGACGGCATCACCCAGGAAAAGATCGACTGGGTGAAGGCGCACAAGACGCACCGCCGCGGGC
GGATCGAGGAGTATTGCGAGGCGTTCAAAGGCGCGAGCTTCACCGCGGGCAAAACGCCGTGGGGCGTGCCGTGCGACGTC
GCGCTGCCGTGCGCGACGCAGAACGAACTGCTCGGCGAGGATGCGAAGACGCTCGTCAGGAACGGCTGCATCGCGGTAAG
CGAGGGCGCCAACATGCCGACGAACCTCGACGGCGTGCATGTCTTCAAGGATGCCAGGATCATGTTTGCACCGGGCAAGG
CGGCGAATGCCGGGGGGGTCGCGGTGTCGGGGCTCGAAATGAGCCAGAACAGCGCGCGGCGCGCGTGGAGTGAAGGCGAG
CTGCAACAGATGCTGAAGGACATCATGGACGGCATCCACGCGCGCTGCCTGACCTATGGCGATCAGGGCAATGGCTATAT
CGACTATGTGAAGGGTGCGAATATCGCGGGGTTCAAGAAGGTTGCCGACGCGATGCTGGCTTTCGGGGTGGTGTAA

Upstream 100 bases:

>100_bases
CTTTGCCGGGCGATGGTGTAGCCTTGGACCCACGAGGTCGGTCAACGACAAAATCGTCAGGGGTCGCACCGGCTTTCCCA
GTCGTACAGGGGAGGGGTGC

Downstream 100 bases:

>100_bases
ATCGGCTCAAGGCGTAAAAGCGCCGGGTTGGGGAAAAGGGGTATTGCGGATGACGGCGCACGCTGGGAAGGAGCGGAGCC
ATCGGCCTTGGGGGGCGTTT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH; NAD(P)H-utilizing glutamate dehydrogenase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAVSDHVDFATFMEGVKRRNPGQPEFVQAVQEVSEDIFDFIADKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNIRVQR
GWRVQNNNAIGPYKGGIRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
GADVDVPAGDIGVGGREIGFMFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLAAKGQDLVGKSAVIS
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGITQEKIDWVKAHKTHRRGRIEEYCEAFKGASFTAGKTPWGVPCDV
ALPCATQNELLGEDAKTLVRNGCIAVSEGANMPTNLDGVHVFKDARIMFAPGKAANAGGVAVSGLEMSQNSARRAWSEGE
LQQMLKDIMDGIHARCLTYGDQGNGYIDYVKGANIAGFKKVADAMLAFGVV

Sequences:

>Translated_451_residues
MAVSDHVDFATFMEGVKRRNPGQPEFVQAVQEVSEDIFDFIADKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNIRVQR
GWRVQNNNAIGPYKGGIRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
GADVDVPAGDIGVGGREIGFMFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLAAKGQDLVGKSAVIS
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGITQEKIDWVKAHKTHRRGRIEEYCEAFKGASFTAGKTPWGVPCDV
ALPCATQNELLGEDAKTLVRNGCIAVSEGANMPTNLDGVHVFKDARIMFAPGKAANAGGVAVSGLEMSQNSARRAWSEGE
LQQMLKDIMDGIHARCLTYGDQGNGYIDYVKGANIAGFKKVADAMLAFGVV
>Mature_450_residues
AVSDHVDFATFMEGVKRRNPGQPEFVQAVQEVSEDIFDFIADKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNIRVQRG
WRVQNNNAIGPYKGGIRFHPSVTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIG
ADVDVPAGDIGVGGREIGFMFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLAAKGQDLVGKSAVISG
SGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGITQEKIDWVKAHKTHRRGRIEEYCEAFKGASFTAGKTPWGVPCDVA
LPCATQNELLGEDAKTLVRNGCIAVSEGANMPTNLDGVHVFKDARIMFAPGKAANAGGVAVSGLEMSQNSARRAWSEGEL
QQMLKDIMDGIHARCLTYGDQGNGYIDYVKGANIAGFKKVADAMLAFGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=355, Percent_Identity=30.9859154929577, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI4885281, Length=355, Percent_Identity=30.7042253521127, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI1788059, Length=441, Percent_Identity=56.0090702947846, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=356, Percent_Identity=30.3370786516854, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=49.1150442477876, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=50, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45549226, Length=385, Percent_Identity=28.051948051948, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45553475, Length=385, Percent_Identity=28.051948051948, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24649283, Length=361, Percent_Identity=28.808864265928, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24649460, Length=290, Percent_Identity=28.9655172413793, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 48889; Mature: 48758

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSDHVDFATFMEGVKRRNPGQPEFVQAVQEVSEDIFDFIADKEEYHAQQILRRIAEPD
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
RVVSFRVCWEDDNGNIRVQRGWRVQNNNAIGPYKGGIRFHPSVTESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIGADVDVPAGDIGVGGREIGF
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLAAKGQDLVGKSAVIS
HHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGITQEKIDWVKAHKTHRRGRIEEYCE
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AFKGASFTAGKTPWGVPCDVALPCATQNELLGEDAKTLVRNGCIAVSEGANMPTNLDGVH
HHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCEEEECCCCCCCCCCCEE
VFKDARIMFAPGKAANAGGVAVSGLEMSQNSARRAWSEGELQQMLKDIMDGIHARCLTYG
EEECCEEEECCCCCCCCCCEEEECCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEC
DQGNGYIDYVKGANIAGFKKVADAMLAFGVV
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVSDHVDFATFMEGVKRRNPGQPEFVQAVQEVSEDIFDFIADKEEYHAQQILRRIAEPD
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
RVVSFRVCWEDDNGNIRVQRGWRVQNNNAIGPYKGGIRFHPSVTESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIGADVDVPAGDIGVGGREIGF
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLAAKGQDLVGKSAVIS
HHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGITQEKIDWVKAHKTHRRGRIEEYCE
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AFKGASFTAGKTPWGVPCDVALPCATQNELLGEDAKTLVRNGCIAVSEGANMPTNLDGVH
HHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHCCCEEEECCCCCCCCCCCEE
VFKDARIMFAPGKAANAGGVAVSGLEMSQNSARRAWSEGELQQMLKDIMDGIHARCLTYG
EEECCEEEECCCCCCCCCCEEEECCHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEC
DQGNGYIDYVKGANIAGFKKVADAMLAFGVV
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955404; 12663928 [H]