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The map label for this gene is 103487758

Identifier: 103487758

GI number: 103487758

Start: 2399025

End: 2400407

Strand: Direct

Name: 103487758

Synonym: Sala_2277

Alternate gene names: NA

Gene position: 2399025-2400407 (Clockwise)

Preceding gene: 103487757

Following gene: 103487759

Centisome position: 71.72

GC content: 67.1

Gene sequence:

>1383_bases
ATGCGCGAAATGAGCACGCGCACCGCTTCGGACCGCCACGATCCCGTCATCGAACATCGCGACCAGCTTGCGGCCCCCAT
GGCCGACGGCGAAAAGCCCAAGGAGCGCTGGCGCATCGGCACCGAGCATGAAAAGTTCGTCTACCGTGTGAGCGATCACC
GCGCGCCCTCCTATGACGAACCCGGCGGCATCCACGACCTCCTGATGGCGCTCACCCGCTTCGGCTGGGAGCCGGTGATC
GAGGGCGGCAAGGTCATCGCGCTGGCGGGCAGCGACGGCACCGTCAGCCTCGAACCCGCGGGGCAACTCGAACTCTCGGG
CGCCCCGCTCGAAAATCTGCACCAGACCTGCGCCGAAACCGGCCGCCATCTGAAGCAGGTGAAGGAAGTCGGCGCCGAAC
TCGGGCTCGGCTTCCTTGGGCTCGGCATGTGGCCCGACAAGAGGCGCGACGAGCTGCCGATCATGCCCAAGGGGCGCTAC
CGGATCATGCTCGATCATATGCCGCGCGTCGGCAGCCTCGGCCTCGACATGATGCTGCGCACCTGCACAATCCAGACCAA
CCTCGACTATGCGAGCGAAGCCGACATGGTGCAGAAGTTCCGCGTGTCGCTCGCGCTCCAGCCGCTCGCGACCGCGCTCT
TCGCCTCGTCGCCCTTCACCGAGGGTCGGCCCAACGGCTATCTGTCGTACCGCAGCCATATCTGGACCGACACCGACCCC
GCGCGCACCGGAATGCTGCCCTTCGTCTTCGAGGATGGTTTCGGCTACGACCGCTATGTCGACTATATGCTCGACGTGCC
GATGTATTTCGTCTTCCGCGACGGCCAATATATCGACGCCGCCGGCCAGAGCTTCCGCGACTTTCTGAAGGGCGAGCTGC
CGGCGCTCCCCGGCGAACTGCCGCGCCTGTCCGACTGGACCGACCATCTCTCGACCGCCTTTCCCGAAGTGCGGCTCAAA
AGCTTCCTCGAAATGCGCGGCGCCGACGGCGGCCCGTGGAACCGCATCTGCGCGCTTCCGGCGCTGTGGGTCGGCCTCCT
CTACGACCAGGACGCACTCGACGCCGCCTGGGATCTCGTCAAGGGGTGGAGCATCGCCGAGCAACAGGCGCTGCGCGACG
CAGTGCCGCGCGAAGGGCTCGACGCGCCCGCGCCCGGCGGCGGCACGGTGGGGCAATTGGCGCACCGCGTGCTCGACATC
GCCGCCGCGGGCCTCGCCGCGCGCGGCGAGGTCAATTCGATGGGCGACAATGAAGTCGGTTTCCTCGAACCGCTGCGCCG
CATCGCGAAAAGCGGCAGATCCCCCGCGCACGACCTGCTCGACCGCTACGAGGATGCGTGGGGCCACGACCTCTCCCGAA
TCTACGACGAGCTGAGCTTCTGA

Upstream 100 bases:

>100_bases
CACAGCCACACTCGCCCCCGGCGGCGCGATGGCGATCGCTTTCACCGTCACGATCGACTGATCGCACGGCCCCGATTGCA
TTCTATACCGGATAGTCTAA

Downstream 100 bases:

>100_bases
CGATCGGGCGGTCGATGGGTATCCGGCGACGCGCCCGTCGCAGCGCTTCCCCGAAGCGCGACCGTCCTTCTGCCGACCGC
TTCCATCACGACCGCGCCAG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MREMSTRTASDRHDPVIEHRDQLAAPMADGEKPKERWRIGTEHEKFVYRVSDHRAPSYDEPGGIHDLLMALTRFGWEPVI
EGGKVIALAGSDGTVSLEPAGQLELSGAPLENLHQTCAETGRHLKQVKEVGAELGLGFLGLGMWPDKRRDELPIMPKGRY
RIMLDHMPRVGSLGLDMMLRTCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTEGRPNGYLSYRSHIWTDTDP
ARTGMLPFVFEDGFGYDRYVDYMLDVPMYFVFRDGQYIDAAGQSFRDFLKGELPALPGELPRLSDWTDHLSTAFPEVRLK
SFLEMRGADGGPWNRICALPALWVGLLYDQDALDAAWDLVKGWSIAEQQALRDAVPREGLDAPAPGGGTVGQLAHRVLDI
AAAGLAARGEVNSMGDNEVGFLEPLRRIAKSGRSPAHDLLDRYEDAWGHDLSRIYDELSF

Sequences:

>Translated_460_residues
MREMSTRTASDRHDPVIEHRDQLAAPMADGEKPKERWRIGTEHEKFVYRVSDHRAPSYDEPGGIHDLLMALTRFGWEPVI
EGGKVIALAGSDGTVSLEPAGQLELSGAPLENLHQTCAETGRHLKQVKEVGAELGLGFLGLGMWPDKRRDELPIMPKGRY
RIMLDHMPRVGSLGLDMMLRTCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTEGRPNGYLSYRSHIWTDTDP
ARTGMLPFVFEDGFGYDRYVDYMLDVPMYFVFRDGQYIDAAGQSFRDFLKGELPALPGELPRLSDWTDHLSTAFPEVRLK
SFLEMRGADGGPWNRICALPALWVGLLYDQDALDAAWDLVKGWSIAEQQALRDAVPREGLDAPAPGGGTVGQLAHRVLDI
AAAGLAARGEVNSMGDNEVGFLEPLRRIAKSGRSPAHDLLDRYEDAWGHDLSRIYDELSF
>Mature_460_residues
MREMSTRTASDRHDPVIEHRDQLAAPMADGEKPKERWRIGTEHEKFVYRVSDHRAPSYDEPGGIHDLLMALTRFGWEPVI
EGGKVIALAGSDGTVSLEPAGQLELSGAPLENLHQTCAETGRHLKQVKEVGAELGLGFLGLGMWPDKRRDELPIMPKGRY
RIMLDHMPRVGSLGLDMMLRTCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTEGRPNGYLSYRSHIWTDTDP
ARTGMLPFVFEDGFGYDRYVDYMLDVPMYFVFRDGQYIDAAGQSFRDFLKGELPALPGELPRLSDWTDHLSTAFPEVRLK
SFLEMRGADGGPWNRICALPALWVGLLYDQDALDAAWDLVKGWSIAEQQALRDAVPREGLDAPAPGGGTVGQLAHRVLDI
AAAGLAARGEVNSMGDNEVGFLEPLRRIAKSGRSPAHDLLDRYEDAWGHDLSRIYDELSF

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51185; Mature: 51185

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREMSTRTASDRHDPVIEHRDQLAAPMADGEKPKERWRIGTEHEKFVYRVSDHRAPSYDE
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHEEEEECCCCCCCCCC
PGGIHDLLMALTRFGWEPVIEGGKVIALAGSDGTVSLEPAGQLELSGAPLENLHQTCAET
CCCHHHHHHHHHHCCCHHHCCCCEEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHH
GRHLKQVKEVGAELGLGFLGLGMWPDKRRDELPIMPKGRYRIMLDHMPRVGSLGLDMMLR
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
TCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTEGRPNGYLSYRSHIWTDTDP
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHCCCCCCCCC
ARTGMLPFVFEDGFGYDRYVDYMLDVPMYFVFRDGQYIDAAGQSFRDFLKGELPALPGEL
HHHCCCCEEEECCCCHHHHHHHHHHCCEEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCC
PRLSDWTDHLSTAFPEVRLKSFLEMRGADGGPWNRICALPALWVGLLYDQDALDAAWDLV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KGWSIAEQQALRDAVPREGLDAPAPGGGTVGQLAHRVLDIAAAGLAARGEVNSMGDNEVG
HCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCC
FLEPLRRIAKSGRSPAHDLLDRYEDAWGHDLSRIYDELSF
HHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MREMSTRTASDRHDPVIEHRDQLAAPMADGEKPKERWRIGTEHEKFVYRVSDHRAPSYDE
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHEEEEECCCCCCCCCC
PGGIHDLLMALTRFGWEPVIEGGKVIALAGSDGTVSLEPAGQLELSGAPLENLHQTCAET
CCCHHHHHHHHHHCCCHHHCCCCEEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHH
GRHLKQVKEVGAELGLGFLGLGMWPDKRRDELPIMPKGRYRIMLDHMPRVGSLGLDMMLR
HHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
TCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTEGRPNGYLSYRSHIWTDTDP
HHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHCCCCCCCCC
ARTGMLPFVFEDGFGYDRYVDYMLDVPMYFVFRDGQYIDAAGQSFRDFLKGELPALPGEL
HHHCCCCEEEECCCCHHHHHHHHHHCCEEEEEECCCEEHHHHHHHHHHHCCCCCCCCCCC
PRLSDWTDHLSTAFPEVRLKSFLEMRGADGGPWNRICALPALWVGLLYDQDALDAAWDLV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KGWSIAEQQALRDAVPREGLDAPAPGGGTVGQLAHRVLDIAAAGLAARGEVNSMGDNEVG
HCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCC
FLEPLRRIAKSGRSPAHDLLDRYEDAWGHDLSRIYDELSF
HHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA