BacMap

Definition	Sphingopyxis alaskensis RB2256, complete genome.
Accession	NC_008048
Length	3,345,170

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The map label for this gene is dnaJ [H]

Identifier: 103487540

GI number: 103487540

Start: 2169178

End: 2170308

Strand: Direct

Name: dnaJ [H]

Synonym: Sala_2059

Alternate gene names: 103487540

Gene position: 2169178-2170308 (Clockwise)

Preceding gene: 103487539

Following gene: 103487543

Centisome position: 64.85

GC content: 67.37

Gene sequence:

>1131_bases
ATGTCGCTCGACCTCGATTATTACGAACTGCTCGAATGCGATCGCAGCGCCGACGAAGCGGCGCTGAAAGCGAGCTACCG
CAAGCTGGCGATGAAATATCACCCCGACAGGAATCCGGGGTGCAAGGACAGCGAAGCGCGCTTCAAGGCGATCAACGAGG
CCTATGACTGCCTTCGCGATCCACAGAGACGCGCCGCCTATGACCGCTTCGGCAAGGCCGGCGTCAATGGCGGCGCGGGC
GGTTTCGGCGGCGGGCATGGCAGCGATTTCGGCGACATCGGCGACATTTTCGAATCGATCTTCGGCTCCGCGTTCGGCGG
CGCGCGCCAGCAGCGCGGCCCGGCGCGCGGCGCCGACCTGCGCTATGACATGGAGATCCGGCTCGAGGACGCCTTCACCG
GCGTGACGCGCGAGATCGAGGTCGATGTCGCCGCGCGCTGCGACGCCTGCGACGGATCGGGTGCGAAGCCCGGCACCCGC
ACGCACCGCTGTTCGACCTGCGGCGGCCACGGCAAGGTGCGCGCGCAGCAGGGATTTTTCATGGTCGAACGCACCTGTCC
CGCCTGTCAGGGCGCGGGCGAAGTCATCGCCGATCCATGCAATAGCTGCCATGGCGAAGGCCGCGTCGACCGGCGCAAGC
GGCTCACCGTCACGATCCCGCCGGGGGTCGACGAAGGCACACGCATCCGCCTGTCGGGCGAGGGCGAAAGCGGCGCGCGC
GGCGCGGCGCCGGGCGACCTTTACATCTTCCTCCACATGGCGCGGCACAAATTGTTCGAGCGCGAAGGCACGACGCTGTT
CACCCGCGCGCCGATCAGTTTCACCACCGCGGCGCTCGGCGGCTGCATCACCATTCCCGGCCTCGACGGCCGCAAGCACG
AAATCGCCATCCCCGCGGGCATCCAGTCGGGCAAGCAGCTCCGCCAGCGCGGCGCGGGGATGCCCGTGCTCAACGGCCGC
GGCCACGGCGACCTGGTCATCCAGATCGACGTCGAAACCCCCACGCGGCTGACCGCGAAGCAGAAGGAATTGCTGCAGGC
GTTCCGCGACACCGAAACCGGCGAGGAATGCCCCGCAAGCCAGGGCTTTTTCGGGCGCATCAAGGATATGTGGGAAGATC
TGACGGATTAG

Upstream 100 bases:

>100_bases
ATGCCAGCGCAGGCTGGCATCGCTCTCGGCAAGGCACCGCCGGACCGGCAGCGACCCCAGCTTTCGCTGGGGTGACGGGT
TGAAGCAGGGGCTTTGACGC

Downstream 100 bases:

>100_bases
GGTCAGATGCGCGCGCAGATGCGTTTGACGGCGGCGAGGTCGTTTTCGGGGCCCTTGTCGTATCGGGTGGCGATGCGTCG
GACGTCTTTGGGACGGCAGA

Product: chaperone protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSLDLDYYELLECDRSADEAALKASYRKLAMKYHPDRNPGCKDSEARFKAINEAYDCLRDPQRRAAYDRFGKAGVNGGAG
GFGGGHGSDFGDIGDIFESIFGSAFGGARQQRGPARGADLRYDMEIRLEDAFTGVTREIEVDVAARCDACDGSGAKPGTR
THRCSTCGGHGKVRAQQGFFMVERTCPACQGAGEVIADPCNSCHGEGRVDRRKRLTVTIPPGVDEGTRIRLSGEGESGAR
GAAPGDLYIFLHMARHKLFEREGTTLFTRAPISFTTAALGGCITIPGLDGRKHEIAIPAGIQSGKQLRQRGAGMPVLNGR
GHGDLVIQIDVETPTRLTAKQKELLQAFRDTETGEECPASQGFFGRIKDMWEDLTD

Sequences:

>Translated_376_residues
MSLDLDYYELLECDRSADEAALKASYRKLAMKYHPDRNPGCKDSEARFKAINEAYDCLRDPQRRAAYDRFGKAGVNGGAG
GFGGGHGSDFGDIGDIFESIFGSAFGGARQQRGPARGADLRYDMEIRLEDAFTGVTREIEVDVAARCDACDGSGAKPGTR
THRCSTCGGHGKVRAQQGFFMVERTCPACQGAGEVIADPCNSCHGEGRVDRRKRLTVTIPPGVDEGTRIRLSGEGESGAR
GAAPGDLYIFLHMARHKLFEREGTTLFTRAPISFTTAALGGCITIPGLDGRKHEIAIPAGIQSGKQLRQRGAGMPVLNGR
GHGDLVIQIDVETPTRLTAKQKELLQAFRDTETGEECPASQGFFGRIKDMWEDLTD
>Mature_375_residues
SLDLDYYELLECDRSADEAALKASYRKLAMKYHPDRNPGCKDSEARFKAINEAYDCLRDPQRRAAYDRFGKAGVNGGAGG
FGGGHGSDFGDIGDIFESIFGSAFGGARQQRGPARGADLRYDMEIRLEDAFTGVTREIEVDVAARCDACDGSGAKPGTRT
HRCSTCGGHGKVRAQQGFFMVERTCPACQGAGEVIADPCNSCHGEGRVDRRKRLTVTIPPGVDEGTRIRLSGEGESGARG
AAPGDLYIFLHMARHKLFEREGTTLFTRAPISFTTAALGGCITIPGLDGRKHEIAIPAGIQSGKQLRQRGAGMPVLNGRG
HGDLVIQIDVETPTRLTAKQKELLQAFRDTETGEECPASQGFFGRIKDMWEDLTD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360838, Length=357, Percent_Identity=32.7731092436975, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI205360840, Length=357, Percent_Identity=32.7731092436975, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI194328760, Length=388, Percent_Identity=32.4742268041237, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI194328758, Length=388, Percent_Identity=32.4742268041237, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5031741, Length=347, Percent_Identity=34.8703170028818, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4504511, Length=372, Percent_Identity=34.1397849462366, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI7706495, Length=347, Percent_Identity=31.700288184438, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI194328762, Length=343, Percent_Identity=31.1953352769679, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI6631085, Length=369, Percent_Identity=26.8292682926829, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI5453690, Length=391, Percent_Identity=24.5524296675192, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI47777312, Length=106, Percent_Identity=45.2830188679245, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI21553335, Length=100, Percent_Identity=40, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI27151736, Length=106, Percent_Identity=38.6792452830189, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI88501736, Length=106, Percent_Identity=38.6792452830189, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI39204547, Length=296, Percent_Identity=26.0135135135135, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI201862518, Length=117, Percent_Identity=40.1709401709402, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI201862587, Length=117, Percent_Identity=40.1709401709402, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI194306642, Length=107, Percent_Identity=43.9252336448598, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI194306640, Length=107, Percent_Identity=43.9252336448598, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI56549115, Length=117, Percent_Identity=40.1709401709402, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI23503241, Length=103, Percent_Identity=39.8058252427184, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI9558755, Length=79, Percent_Identity=45.5696202531646, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI217035105, Length=73, Percent_Identity=49.3150684931507, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4885495, Length=69, Percent_Identity=47.8260869565217, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI68077166, Length=101, Percent_Identity=41.5841584158416, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI68077168, Length=101, Percent_Identity=41.5841584158416, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI17388799, Length=69, Percent_Identity=47.8260869565217, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI45504382, Length=68, Percent_Identity=44.1176470588235, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI24308127, Length=78, Percent_Identity=37.1794871794872, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI56687498, Length=114, Percent_Identity=35.0877192982456, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI22749377, Length=106, Percent_Identity=40.5660377358491, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786197, Length=374, Percent_Identity=50.8021390374332, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787235, Length=352, Percent_Identity=30.6818181818182, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553098, Length=365, Percent_Identity=32.6027397260274, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17553096, Length=356, Percent_Identity=32.8651685393258, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17507263, Length=341, Percent_Identity=31.0850439882698, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI212645553, Length=348, Percent_Identity=32.183908045977, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17563890, Length=226, Percent_Identity=29.646017699115, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554644, Length=99, Percent_Identity=39.3939393939394, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534355, Length=134, Percent_Identity=35.8208955223881, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115532378, Length=82, Percent_Identity=42.6829268292683, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554874, Length=77, Percent_Identity=41.5584415584416, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538236, Length=112, Percent_Identity=39.2857142857143, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324265, Length=352, Percent_Identity=33.5227272727273, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323870, Length=354, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324252, Length=431, Percent_Identity=25.5220417633411, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321094, Length=393, Percent_Identity=32.0610687022901, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323119, Length=382, Percent_Identity=26.7015706806283, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6323813, Length=111, Percent_Identity=41.4414414414414, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320888, Length=102, Percent_Identity=36.2745098039216, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322194, Length=102, Percent_Identity=36.2745098039216, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324321, Length=71, Percent_Identity=47.887323943662, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324103, Length=71, Percent_Identity=43.6619718309859, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45552813, Length=382, Percent_Identity=29.5811518324607, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45549272, Length=382, Percent_Identity=29.5811518324607, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45552811, Length=382, Percent_Identity=29.5811518324607, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24646562, Length=346, Percent_Identity=32.3699421965318, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646560, Length=346, Percent_Identity=32.3699421965318, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646558, Length=346, Percent_Identity=32.3699421965318, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646556, Length=346, Percent_Identity=32.3699421965318, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21357547, Length=346, Percent_Identity=32.3699421965318, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI20129487, Length=354, Percent_Identity=30.7909604519774, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24584014, Length=354, Percent_Identity=30.7909604519774, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI85726398, Length=370, Percent_Identity=26.7567567567568, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19920464, Length=349, Percent_Identity=29.512893982808, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24658562, Length=361, Percent_Identity=28.808864265928, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24658555, Length=361, Percent_Identity=28.808864265928, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18858081, Length=357, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574949, Length=364, Percent_Identity=23.9010989010989, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24654066, Length=72, Percent_Identity=45.8333333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654070, Length=72, Percent_Identity=45.8333333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654068, Length=72, Percent_Identity=45.8333333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654072, Length=72, Percent_Identity=45.8333333333333, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24651439, Length=79, Percent_Identity=39.2405063291139, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24651436, Length=75, Percent_Identity=41.3333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21358311, Length=75, Percent_Identity=41.3333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24762799, Length=102, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161077138, Length=65, Percent_Identity=46.1538461538462, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17737735, Length=68, Percent_Identity=42.6470588235294, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28574723, Length=68, Percent_Identity=42.6470588235294, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24668523, Length=68, Percent_Identity=42.6470588235294, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 40658; Mature: 40527

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2 ; PS00637 DNAJ_CXXCXGXG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDLDYYELLECDRSADEAALKASYRKLAMKYHPDRNPGCKDSEARFKAINEAYDCLRD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PQRRAAYDRFGKAGVNGGAGGFGGGHGSDFGDIGDIFESIFGSAFGGARQQRGPARGADL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCE
RYDMEIRLEDAFTGVTREIEVDVAARCDACDGSGAKPGTRTHRCSTCGGHGKVRAQQGFF
EEEEEEEEEHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEE
MVERTCPACQGAGEVIADPCNSCHGEGRVDRRKRLTVTIPPGVDEGTRIRLSGEGESGAR
EEECCCCCCCCCCHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCC
GAAPGDLYIFLHMARHKLFEREGTTLFTRAPISFTTAALGGCITIPGLDGRKHEIAIPAG
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCEEECCCCCCCCEEEEECCC
IQSGKQLRQRGAGMPVLNGRGHGDLVIQIDVETPTRLTAKQKELLQAFRDTETGEECPAS
CHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
QGFFGRIKDMWEDLTD
CCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLDLDYYELLECDRSADEAALKASYRKLAMKYHPDRNPGCKDSEARFKAINEAYDCLRD
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PQRRAAYDRFGKAGVNGGAGGFGGGHGSDFGDIGDIFESIFGSAFGGARQQRGPARGADL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCE
RYDMEIRLEDAFTGVTREIEVDVAARCDACDGSGAKPGTRTHRCSTCGGHGKVRAQQGFF
EEEEEEEEEHHHCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEE
MVERTCPACQGAGEVIADPCNSCHGEGRVDRRKRLTVTIPPGVDEGTRIRLSGEGESGAR
EEECCCCCCCCCCHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCC
GAAPGDLYIFLHMARHKLFEREGTTLFTRAPISFTTAALGGCITIPGLDGRKHEIAIPAG
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCEEECCCCCCCCEEEEECCC
IQSGKQLRQRGAGMPVLNGRGHGDLVIQIDVETPTRLTAKQKELLQAFRDTETGEECPAS
CHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
QGFFGRIKDMWEDLTD
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA