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The map label for this gene is rpoH [H]

Identifier: 103487228

GI number: 103487228

Start: 1836030

End: 1836932

Strand: Direct

Name: rpoH [H]

Synonym: Sala_1743

Alternate gene names: 103487228

Gene position: 1836030-1836932 (Clockwise)

Preceding gene: 103487227

Following gene: 103487229

Centisome position: 54.89

GC content: 62.79

Gene sequence:

>903_bases
ATGGCTAACAAAAGCAATGTTCCGGCCGTGGTCCCAGCGCTTGGCGGCGAAGCCAGCCTGAACCGCTATCTGGCCGAAAT
TCGCAAATTCCCCCTTCTGACGCCCGAGCAGGAATATATGCTCGCCAAGCGCTTTCAGGAACATGGCGACAATGAAGCGG
CGGCGCAGCTCGTCACCTCGCACCTCCGCCTCGTCGCCAAGATCGCGATGGGCTATCGCGGCTATGGCCTGCCCGTCAGC
GAACTGATCAGCGAAGGCAATATCGGCCTGATGCAGGGGGTGAAGAAATTCGACCCCGAACGCGGCTTCCGCCTTGCCAC
CTATGCGATGTGGTGGATTCGCGCGTCGATCCAGGAGTTCATCCTTCGCTCGTGGAGCCTCGTCAAAATGGGCACGACGG
CGGCGCAGAAAAAGCTGTTCTTCAATCTCCGCCGGATGAAGAACAACCTCGAAGCGTTCGAGGACGGCGACCTCAGCCCC
GAACATGTCGCCAAGATCGCGACCGACCTTGGCGTGACCGAGGACGAGGTGGTCAGCATGAACCGCCGCATGGCGATGGG
CGGCGACACCTCGCTCAACGTCCCGATGGGCGAGGATGGCGACAGCCAGTGGCAGGATCTGCTGGGCGACGACGGCCCGT
TGCAGGACGAGCGTGTCGCCGAGGCGCAGGAGCGCGACGTGCGGCACGACCTGCTGACCGAAGCGCTCGAAACGCTCAAC
GAGCGCGAACGTCACATCCTGACCGAACGCCGCCTGACTGACGACCCCAAGACGCTCGAGGATCTGAGCCAGCTTTACGA
CGTCAGCCGCGAGCGCGTCCGCCAGATCGAGGTGCGCGCCTTTGAAAAGCTGCAAAAGGCGATGCTCAAGCTCGCTGGCG
ACCGGCGCCTCCTGACTGCCTGA

Upstream 100 bases:

>100_bases
ATCGATGAACTGCGCGTTTAGGTTTCGAATGAAAATCTATTTTGACCGGCGGACTGAACCGCGGCAAGATGTTTTCAGGA
TTTAGGGAAGACTCTCTCCG

Downstream 100 bases:

>100_bases
CGCCGGACAGCGCGGATGATGCGGCGCAGCGCGGATAGCGGTCTATGATCCGTCGCCGCGCCCCCGCCACCCGCATTGGA
TGGAGCGCGGTGATACTGGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MANKSNVPAVVPALGGEASLNRYLAEIRKFPLLTPEQEYMLAKRFQEHGDNEAAAQLVTSHLRLVAKIAMGYRGYGLPVS
ELISEGNIGLMQGVKKFDPERGFRLATYAMWWIRASIQEFILRSWSLVKMGTTAAQKKLFFNLRRMKNNLEAFEDGDLSP
EHVAKIATDLGVTEDEVVSMNRRMAMGGDTSLNVPMGEDGDSQWQDLLGDDGPLQDERVAEAQERDVRHDLLTEALETLN
ERERHILTERRLTDDPKTLEDLSQLYDVSRERVRQIEVRAFEKLQKAMLKLAGDRRLLTA

Sequences:

>Translated_300_residues
MANKSNVPAVVPALGGEASLNRYLAEIRKFPLLTPEQEYMLAKRFQEHGDNEAAAQLVTSHLRLVAKIAMGYRGYGLPVS
ELISEGNIGLMQGVKKFDPERGFRLATYAMWWIRASIQEFILRSWSLVKMGTTAAQKKLFFNLRRMKNNLEAFEDGDLSP
EHVAKIATDLGVTEDEVVSMNRRMAMGGDTSLNVPMGEDGDSQWQDLLGDDGPLQDERVAEAQERDVRHDLLTEALETLN
ERERHILTERRLTDDPKTLEDLSQLYDVSRERVRQIEVRAFEKLQKAMLKLAGDRRLLTA
>Mature_299_residues
ANKSNVPAVVPALGGEASLNRYLAEIRKFPLLTPEQEYMLAKRFQEHGDNEAAAQLVTSHLRLVAKIAMGYRGYGLPVSE
LISEGNIGLMQGVKKFDPERGFRLATYAMWWIRASIQEFILRSWSLVKMGTTAAQKKLFFNLRRMKNNLEAFEDGDLSPE
HVAKIATDLGVTEDEVVSMNRRMAMGGDTSLNVPMGEDGDSQWQDLLGDDGPLQDERVAEAQERDVRHDLLTEALETLNE
RERHILTERRLTDDPKTLEDLSQLYDVSRERVRQIEVRAFEKLQKAMLKLAGDRRLLTA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=291, Percent_Identity=40.5498281786942, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=36.1313868613139, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=32.6359832635983, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34030; Mature: 33899

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKSNVPAVVPALGGEASLNRYLAEIRKFPLLTPEQEYMLAKRFQEHGDNEAAAQLVTS
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
HLRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFDPERGFRLATYAMWWIRASIQEF
HHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ILRSWSLVKMGTTAAQKKLFFNLRRMKNNLEAFEDGDLSPEHVAKIATDLGVTEDEVVSM
HHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHH
NRRMAMGGDTSLNVPMGEDGDSQWQDLLGDDGPLQDERVAEAQERDVRHDLLTEALETLN
HHHHCCCCCCEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERERHILTERRLTDDPKTLEDLSQLYDVSRERVRQIEVRAFEKLQKAMLKLAGDRRLLTA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ANKSNVPAVVPALGGEASLNRYLAEIRKFPLLTPEQEYMLAKRFQEHGDNEAAAQLVTS
CCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
HLRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFDPERGFRLATYAMWWIRASIQEF
HHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ILRSWSLVKMGTTAAQKKLFFNLRRMKNNLEAFEDGDLSPEHVAKIATDLGVTEDEVVSM
HHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHH
NRRMAMGGDTSLNVPMGEDGDSQWQDLLGDDGPLQDERVAEAQERDVRHDLLTEALETLN
HHHHCCCCCCEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERERHILTERRLTDDPKTLEDLSQLYDVSRERVRQIEVRAFEKLQKAMLKLAGDRRLLTA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]