Click here to switch to the map view.

The map label for this gene is celR [H]

Identifier: 103485798

GI number: 103485798

Start: 302837

End: 303844

Strand: Direct

Name: celR [H]

Synonym: Sala_0303

Alternate gene names: 103485798

Gene position: 302837-303844 (Clockwise)

Preceding gene: 103485797

Following gene: 103485799

Centisome position: 9.05

GC content: 69.05

Gene sequence:

>1008_bases
ATGGCAAAGGGATCGGGGTCCAATGGCGGCGGCAACGCGCGCCCGACGATGGCGGACATCGCGAACGAACTCGGTCTTGC
CAAGATCACCGTCTCGCGCGCGCTGTCGAACCCGTCGAGCGTCAAGGAAAGCACGCGGCGGCTGGTCCGTGAAACCGCCG
AGCGCATGGGATATCGGCTCAACGTATCGGCGCGCAACCTGCGCCAACAGCGTACGCGCACGATCGCGGTGGTGATCGAA
ATGACGCCGTCGCACGAACGCTTGATGAGCGAGCCCTATCCGCTGCTGCTGCTCGGCGGCATCATGCAGGAACTGACCGC
GGCCGGGCAGAATATGGTGCTGACGACGATCGACCTGTTCACCGCGGCGCCGCCCTCGGCCGATGGTGTCATCCTGCTGG
GGCAGGGGGTGCATGACGATGCCGCCGCAGTGATCGAAGCGACCGGGCTGCCCTATGTGGTTTGGGGTGCCGAACATGGC
CCGCCGGGGCGCATCGTCGTCGGCAGCGACAACCGCGAGGGCGGACGGCTCGCTGCCGACCATCTGCTCGGCCGCGGATG
CCGCCGCCTCCTGTTCCTCGGCGATGCCGAACATGGCGAGGCCGAGGACCGGCTGCGCGGGTTCGAGGCGCGCGTCGCGG
CGTCGGACGCCGCGATCGTCGGCAATATCCCCTGCGCCTTCACCCTGCCCGCGGGGCGCGAAGCGGTAGGGCCGCTGCTC
GACGCGCGCGGCCTCGATTTCGACGGCATATTTGCGGCGAGCGATGCGATCGCGATGGGCGCGATCGAGGCGTTGCAGGC
GCGCGGCCACCGTGTGCCGGGCGAGGTGTCGGTCGTCGGCTTCGACGATTCGCCCGGCGCCGCGCTCTTTTCGCCGTCGC
TCACCAGCATCCGGCAGGACTGGGCGGTGGGCGGCGAACTGCTGGCACAAAAAGTGCTCGCCAAGGTGGCGGGCCAGCCG
GTCGAGTCGCGCGCGATGCCGGTGGTTCTGGTCAAGCGCGAAAGCTGA

Upstream 100 bases:

>100_bases
TGAACACGCCGTGGCAGGACGGGCAGGATTATATCTATTGGACCAACTGATCGCGTCTGACCGCTTGTGAAACAAGGCCG
CCGCACGTTAAGGGGCGGGG

Downstream 100 bases:

>100_bases
TATTTCAGACATTTTCCCGGTCGGTCACGCGCCTTTGACCGCGAAATGGTATCGATACCTTGACATCGATACCATTTCGC
GCGAAACAGCATCGAGAGCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAKGSGSNGGGNARPTMADIANELGLAKITVSRALSNPSSVKESTRRLVRETAERMGYRLNVSARNLRQQRTRTIAVVIE
MTPSHERLMSEPYPLLLLGGIMQELTAAGQNMVLTTIDLFTAAPPSADGVILLGQGVHDDAAAVIEATGLPYVVWGAEHG
PPGRIVVGSDNREGGRLAADHLLGRGCRRLLFLGDAEHGEAEDRLRGFEARVAASDAAIVGNIPCAFTLPAGREAVGPLL
DARGLDFDGIFAASDAIAMGAIEALQARGHRVPGEVSVVGFDDSPGAALFSPSLTSIRQDWAVGGELLAQKVLAKVAGQP
VESRAMPVVLVKRES

Sequences:

>Translated_335_residues
MAKGSGSNGGGNARPTMADIANELGLAKITVSRALSNPSSVKESTRRLVRETAERMGYRLNVSARNLRQQRTRTIAVVIE
MTPSHERLMSEPYPLLLLGGIMQELTAAGQNMVLTTIDLFTAAPPSADGVILLGQGVHDDAAAVIEATGLPYVVWGAEHG
PPGRIVVGSDNREGGRLAADHLLGRGCRRLLFLGDAEHGEAEDRLRGFEARVAASDAAIVGNIPCAFTLPAGREAVGPLL
DARGLDFDGIFAASDAIAMGAIEALQARGHRVPGEVSVVGFDDSPGAALFSPSLTSIRQDWAVGGELLAQKVLAKVAGQP
VESRAMPVVLVKRES
>Mature_334_residues
AKGSGSNGGGNARPTMADIANELGLAKITVSRALSNPSSVKESTRRLVRETAERMGYRLNVSARNLRQQRTRTIAVVIEM
TPSHERLMSEPYPLLLLGGIMQELTAAGQNMVLTTIDLFTAAPPSADGVILLGQGVHDDAAAVIEATGLPYVVWGAEHGP
PGRIVVGSDNREGGRLAADHLLGRGCRRLLFLGDAEHGEAEDRLRGFEARVAASDAAIVGNIPCAFTLPAGREAVGPLLD
ARGLDFDGIFAASDAIAMGAIEALQARGHRVPGEVSVVGFDDSPGAALFSPSLTSIRQDWAVGGELLAQKVLAKVAGQPV
ESRAMPVVLVKRES

Specific function: Binds to the 14-bp operator sequence 5'-TGGGAGCGCTCCCA- 3' in the upstream regions of cellulase genes; these operator sequences are modulated by cellobiose [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=29.6666666666667, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789202, Length=317, Percent_Identity=26.813880126183, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788474, Length=339, Percent_Identity=27.4336283185841, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=25.2252252252252, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=25.5384615384615, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=26.7692307692308, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=26.6862170087977, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789456, Length=326, Percent_Identity=26.0736196319018, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=25.1515151515152, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790192, Length=268, Percent_Identity=26.4925373134328, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35122; Mature: 34990

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGSGSNGGGNARPTMADIANELGLAKITVSRALSNPSSVKESTRRLVRETAERMGYRL
CCCCCCCCCCCCCCCHHHHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHCCEEE
NVSARNLRQQRTRTIAVVIEMTPSHERLMSEPYPLLLLGGIMQELTAAGQNMVLTTIDLF
ECHHHHHHHHCCEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHH
TAAPPSADGVILLGQGVHDDAAAVIEATGLPYVVWGAEHGPPGRIVVGSDNREGGRLAAD
HCCCCCCCCEEEEECCCCCCHHHHEEECCCCEEEECCCCCCCCEEEECCCCCCCCCHHHH
HLLGRGCRRLLFLGDAEHGEAEDRLRGFEARVAASDAAIVGNIPCAFTLPAGREAVGPLL
HHHHHHHHEEEEECCCCCCCHHHHHHCHHHEEECCCCEEEECCCEEEECCCCCHHHHHHH
DARGLDFDGIFAASDAIAMGAIEALQARGHRVPGEVSVVGFDDSPGAALFSPSLTSIRQD
HCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHH
WAVGGELLAQKVLAKVAGQPVESRAMPVVLVKRES
HHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEEECC
>Mature Secondary Structure 
AKGSGSNGGGNARPTMADIANELGLAKITVSRALSNPSSVKESTRRLVRETAERMGYRL
CCCCCCCCCCCCCCHHHHHHHHCCHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHCCEEE
NVSARNLRQQRTRTIAVVIEMTPSHERLMSEPYPLLLLGGIMQELTAAGQNMVLTTIDLF
ECHHHHHHHHCCEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHH
TAAPPSADGVILLGQGVHDDAAAVIEATGLPYVVWGAEHGPPGRIVVGSDNREGGRLAAD
HCCCCCCCCEEEEECCCCCCHHHHEEECCCCEEEECCCCCCCCEEEECCCCCCCCCHHHH
HLLGRGCRRLLFLGDAEHGEAEDRLRGFEARVAASDAAIVGNIPCAFTLPAGREAVGPLL
HHHHHHHHEEEEECCCCCCCHHHHHHCHHHEEECCCCEEEECCCEEEECCCCCHHHHHHH
DARGLDFDGIFAASDAIAMGAIEALQARGHRVPGEVSVVGFDDSPGAALFSPSLTSIRQD
HCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHH
WAVGGELLAQKVLAKVAGQPVESRAMPVVLVKRES
HHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10224066 [H]