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The map label for this gene is pspA [H]

Identifier: 103485703

GI number: 103485703

Start: 221379

End: 222041

Strand: Direct

Name: pspA [H]

Synonym: Sala_0207

Alternate gene names: 103485703

Gene position: 221379-222041 (Clockwise)

Preceding gene: 103485702

Following gene: 103485704

Centisome position: 6.62

GC content: 62.14

Gene sequence:

>663_bases
ATGGGTATTTTTTCACGAACCCGCGACATCATCGCCGCCAACGTCACCGACCTGCTCGACCGGGCCGAGGATCCTGAAAA
GATGATCCGCCAGATCATCTTCGAGATGAACGAAACGCTTGTCGAAGTTCGCGCTTCCGCCGCCCGCACCATTGCGGACC
AGAAGGAGATGCGCCGCCACATCGCCAAACTCGAGAGTCTGCAGGACAGCTGGAAGGAAAAGGCCGAACTCGCGCTGTCG
AAGGACCGCGAAGACCTCGCCACCGCCGCGCTCGTCGAAAAGCAGAAGGCCGGCGACATGGCCGAGCGACTCAAGGCCGA
GATCGCCGTCCTCGACGACGCGCTGTCCGGTTATGAAGCCGACATCGCCAAGCTTCAGAAAAAGCTGTCGGAAGCGCGCG
CGCGCCAGTCGAATATCGTCAACCGCCTCGAAAGCGCCGAGAACCGCTATCGCCTCCGCGAAATGACCAACGGCGACCGC
GTCGAAGACGCTTTCTCGCGCTTCGAGATCCTTGAGCGCCGCGTCGACGAAGCCGAGGGCCGGGCCGATGCGCTGGGTCT
CGGTTACAAGAAGTCGCTCGACGAAGAGATCGCCGAACTTCAGGCTGCCGACAAGGTCGCCGACGAGCTCGCCGCGCTGA
AGGCCGCGCAGGCCAAGAAATAA

Upstream 100 bases:

>100_bases
CGGCACTGGTGCCGGGGCACTCTCCCCCCTTGCCCCGCCCCGGCACCAGCCTTTTTTGAACAAGACAACCAGGTCTCAAG
TTTTCGACAGGAGTTTCCCA

Downstream 100 bases:

>100_bases
GGAGCCGGACCGATGGAAGAAGTCATCATCGTCCCGATCGTCATCGGAACGCTCTTCCTCGGTCTGCCCTGGCTGATCCT
CCACTATATCACCAAGTGGA

Product: phage shock protein A, PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MGIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVRASAARTIADQKEMRRHIAKLESLQDSWKEKAELALS
KDREDLATAALVEKQKAGDMAERLKAEIAVLDDALSGYEADIAKLQKKLSEARARQSNIVNRLESAENRYRLREMTNGDR
VEDAFSRFEILERRVDEAEGRADALGLGYKKSLDEEIAELQAADKVADELAALKAAQAKK

Sequences:

>Translated_220_residues
MGIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVRASAARTIADQKEMRRHIAKLESLQDSWKEKAELALS
KDREDLATAALVEKQKAGDMAERLKAEIAVLDDALSGYEADIAKLQKKLSEARARQSNIVNRLESAENRYRLREMTNGDR
VEDAFSRFEILERRVDEAEGRADALGLGYKKSLDEEIAELQAADKVADELAALKAAQAKK
>Mature_219_residues
GIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVRASAARTIADQKEMRRHIAKLESLQDSWKEKAELALSK
DREDLATAALVEKQKAGDMAERLKAEIAVLDDALSGYEADIAKLQKKLSEARARQSNIVNRLESAENRYRLREMTNGDRV
EDAFSRFEILERRVDEAEGRADALGLGYKKSLDEEIAELQAADKVADELAALKAAQAKK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=215, Percent_Identity=45.5813953488372, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 24762; Mature: 24631

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVRASAARTIADQKEMRRH
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLESLQDSWKEKAELALSKDREDLATAALVEKQKAGDMAERLKAEIAVLDDALSGYEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
DIAKLQKKLSEARARQSNIVNRLESAENRYRLREMTNGDRVEDAFSRFEILERRVDEAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
RADALGLGYKKSLDEEIAELQAADKVADELAALKAAQAKK
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVRASAARTIADQKEMRRH
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLESLQDSWKEKAELALSKDREDLATAALVEKQKAGDMAERLKAEIAVLDDALSGYEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
DIAKLQKKLSEARARQSNIVNRLESAENRYRLREMTNGDRVEDAFSRFEILERRVDEAEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
RADALGLGYKKSLDEEIAELQAADKVADELAALKAAQAKK
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]