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The map label for this gene is ada [H]

Identifier: 158340505

GI number: 158340505

Start: 54103

End: 54990

Strand: Direct

Name: ada [H]

Synonym: AM1_C0050

Alternate gene names: 158340505

Gene position: 54103-54990 (Clockwise)

Preceding gene: 158340502

Following gene: 158340506

Centisome position: 19.81

GC content: 47.97

Gene sequence:

>888_bases
ATGGGTCTACGAAGACTTACCCCGGAAGACGACCCGTCAGACCACTATCAGATGGTCGCAGCAGCAATCGCTTTTATTCG
TGAGCATCGCTTTGAGCAGCCAGCCTTATCTACCGTTGCTCAGCACATCGGACTGAGTGAGTACCACTTTCAAAGGCTAT
TTACCTATTGGGCAGGTATTAGCCCAAAGCGGTTTCTGCAATATCTGACGATTGAGCAAGCCAAAGCGACTATTGCTCAA
ACGGATAGTTTACTTACACTCAGCGAAGATCTAGGTCTTTCAGGCCCTAGCCGTTTACACGATCTGTTCGTTTCTATAGA
AGCGATGTCACCTGGCGAATATAAAAATGGTGGCGAAGGACTAGAAATATACAACGGTTCGCACAACACCTTGTTTGGAA
CCGTTGTGATCGCCACAACAAAAAGAGGTATCTGCTCACTTCAGTTTCAAGACAAGGCCACGCTGCCTGTAGACGACTTG
CTAACCAATACTTGGCCGAATGCCACGTTGACTGTGGATCAGGAGAAAACACAGGGGTTGTGTGATCGCATCTTTTTACC
AGAGATGTCTCAGCCGACTAAGCCCTTGACACTGCTAGTTAAGGGAACAAACTTTCAAGTCCAAGTCTGGCGAGCGTTGC
TTCAAATTCCACTAGGTGGTCTCACAACTTACCAAACAATCGCTGAGATGATCGAGCGACCTAAAGCTGCTAGGGCTGTC
GGTACTGCCATTGGCAAGAATCCGATTGGCTATCTCATTCCTTGTCATCGTGTTATTCGAGGCTCAGGAGAGATCGGTGG
CTACAGTTGGGGACTAGATCGCAAGCAAGCCATCTTAGGTTGGGAAGCTGCCCAACTCAATCATTCAACCATACAGGAAG
GAAGCTAA

Upstream 100 bases:

>100_bases
CAGTGAAGCCTGTCCACTATCTAATCATCGCAAGAATCGGGTGGCTCATTTGTTCATAGCTTTTGTAAGCTAATGCGAAC
ACATCCTAGAGAAAACTGGT

Downstream 100 bases:

>100_bases
TGCGGTTATCGGATGTCCTTGAACTTCTTCTGCTATCTGCGCTATGGGGCGGTTCGTTTTTGTTCATGCGCATAGCGGCA
CCCGTACTAGGCCCAATCTG

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MGLRRLTPEDDPSDHYQMVAAAIAFIREHRFEQPALSTVAQHIGLSEYHFQRLFTYWAGISPKRFLQYLTIEQAKATIAQ
TDSLLTLSEDLGLSGPSRLHDLFVSIEAMSPGEYKNGGEGLEIYNGSHNTLFGTVVIATTKRGICSLQFQDKATLPVDDL
LTNTWPNATLTVDQEKTQGLCDRIFLPEMSQPTKPLTLLVKGTNFQVQVWRALLQIPLGGLTTYQTIAEMIERPKAARAV
GTAIGKNPIGYLIPCHRVIRGSGEIGGYSWGLDRKQAILGWEAAQLNHSTIQEGS

Sequences:

>Translated_295_residues
MGLRRLTPEDDPSDHYQMVAAAIAFIREHRFEQPALSTVAQHIGLSEYHFQRLFTYWAGISPKRFLQYLTIEQAKATIAQ
TDSLLTLSEDLGLSGPSRLHDLFVSIEAMSPGEYKNGGEGLEIYNGSHNTLFGTVVIATTKRGICSLQFQDKATLPVDDL
LTNTWPNATLTVDQEKTQGLCDRIFLPEMSQPTKPLTLLVKGTNFQVQVWRALLQIPLGGLTTYQTIAEMIERPKAARAV
GTAIGKNPIGYLIPCHRVIRGSGEIGGYSWGLDRKQAILGWEAAQLNHSTIQEGS
>Mature_294_residues
GLRRLTPEDDPSDHYQMVAAAIAFIREHRFEQPALSTVAQHIGLSEYHFQRLFTYWAGISPKRFLQYLTIEQAKATIAQT
DSLLTLSEDLGLSGPSRLHDLFVSIEAMSPGEYKNGGEGLEIYNGSHNTLFGTVVIATTKRGICSLQFQDKATLPVDDLL
TNTWPNATLTVDQEKTQGLCDRIFLPEMSQPTKPLTLLVKGTNFQVQVWRALLQIPLGGLTTYQTIAEMIERPKAARAVG
TAIGKNPIGYLIPCHRVIRGSGEIGGYSWGLDRKQAILGWEAAQLNHSTIQEGS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=85, Percent_Identity=44.7058823529412, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788542, Length=252, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=50, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=89, Percent_Identity=41.5730337078652, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=41.5730337078652, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=41.9753086419753, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137554, Length=143, Percent_Identity=33.5664335664336, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32625; Mature: 32493

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRRLTPEDDPSDHYQMVAAAIAFIREHRFEQPALSTVAQHIGLSEYHFQRLFTYWAGI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
SPKRFLQYLTIEQAKATIAQTDSLLTLSEDLGLSGPSRLHDLFVSIEAMSPGEYKNGGEG
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHEEEECCCCCCCCCCCC
LEIYNGSHNTLFGTVVIATTKRGICSLQFQDKATLPVDDLLTNTWPNATLTVDQEKTQGL
EEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCHHHHHH
CDRIFLPEMSQPTKPLTLLVKGTNFQVQVWRALLQIPLGGLTTYQTIAEMIERPKAARAV
HHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHH
GTAIGKNPIGYLIPCHRVIRGSGEIGGYSWGLDRKQAILGWEAAQLNHSTIQEGS
HHHHCCCCCCEEECHHHHHCCCCCCCCEECCCCCHHHHHCCHHHHCCCCHHCCCC
>Mature Secondary Structure 
GLRRLTPEDDPSDHYQMVAAAIAFIREHRFEQPALSTVAQHIGLSEYHFQRLFTYWAGI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
SPKRFLQYLTIEQAKATIAQTDSLLTLSEDLGLSGPSRLHDLFVSIEAMSPGEYKNGGEG
CHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHEEEECCCCCCCCCCCC
LEIYNGSHNTLFGTVVIATTKRGICSLQFQDKATLPVDDLLTNTWPNATLTVDQEKTQGL
EEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCCCEEEECCHHHHHH
CDRIFLPEMSQPTKPLTLLVKGTNFQVQVWRALLQIPLGGLTTYQTIAEMIERPKAARAV
HHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHH
GTAIGKNPIGYLIPCHRVIRGSGEIGGYSWGLDRKQAILGWEAAQLNHSTIQEGS
HHHHCCCCCCEEECHHHHHCCCCCCCCEECCCCCHHHHHCCHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]