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The map label for this gene is yfiK [H]

Identifier: 158340472

GI number: 158340472

Start: 15735

End: 16382

Strand: Direct

Name: yfiK [H]

Synonym: AM1_C0017

Alternate gene names: 158340472

Gene position: 15735-16382 (Clockwise)

Preceding gene: 158340471

Following gene: 158340473

Centisome position: 5.76

GC content: 47.53

Gene sequence:

>648_bases
ATGGCTATTCAAATTTTGTTGGTCGATGATCAATACCTGATCCGGGAAGGGATTGCCTCCTTGTTAGAACTGGAAGAGGA
TCTGGAGGTAGCGGGGATGGCAGCTAATGGTAAAGAGGCCATAAAACAAGCCATTGACCTGAAACCCAATATCATCCTGA
TGGATATCAGAATGCCTGAACTGAATGGGGTTGAGGCAACGGCCAAAATCAAGCAATCACTCCCCCATTGTCAAATCATT
ATGTTGACCACATTCGATGATGAAGAGCTAATTGTGCAGTCGCTACTGGCAGGGGCGTGTGGTTATCTGATGAAGGACAT
TCCCCCGGACGATCTTGGGCAAGCGATTAAGTTGGCCGATGCGGGCGTGTTTCAGCTCGCACCTGAGGTTGCTGGTAAAT
TAATTGGTTCACTCAAAACTCAACGGGCTGGTGACGCTCCCAAACTTCCTACCGATACACCACTGACAACCAGAGAACTC
GAAGTTTTAAAGCTGCTCGCCAAAGGCAACACGAATAAAGAAATTGCCAAAGCGTTTAACTTGAGTGAGGGAACCGTCAA
AAACCACGTTTCCAATATTTTAATGAGACTTGGACTCAGAGACCGCACCCAGGCAGCCGTTTACGCAGTAGAGCATGGCT
TGAGTTGA

Upstream 100 bases:

>100_bases
GTGGAACCCTCGATATCACCAGTCATTCCCAAGCCACAACCCTCGCTATCAATATCCCCCAAGACCTTCCTCAGATCAAT
TACCGGGCTGAGTAAACGCT

Downstream 100 bases:

>100_bases
AACGAAATAGAGGGTCACGGGACCCAATCTGACTACCTATTCATCATTACCTCTCAGTCTATTGGTACTTTTTGCCTAAA
TTGATTTATGTCAAAAGAGC

Product: two-component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MAIQILLVDDQYLIREGIASLLELEEDLEVAGMAANGKEAIKQAIDLKPNIILMDIRMPELNGVEATAKIKQSLPHCQII
MLTTFDDEELIVQSLLAGACGYLMKDIPPDDLGQAIKLADAGVFQLAPEVAGKLIGSLKTQRAGDAPKLPTDTPLTTREL
EVLKLLAKGNTNKEIAKAFNLSEGTVKNHVSNILMRLGLRDRTQAAVYAVEHGLS

Sequences:

>Translated_215_residues
MAIQILLVDDQYLIREGIASLLELEEDLEVAGMAANGKEAIKQAIDLKPNIILMDIRMPELNGVEATAKIKQSLPHCQII
MLTTFDDEELIVQSLLAGACGYLMKDIPPDDLGQAIKLADAGVFQLAPEVAGKLIGSLKTQRAGDAPKLPTDTPLTTREL
EVLKLLAKGNTNKEIAKAFNLSEGTVKNHVSNILMRLGLRDRTQAAVYAVEHGLS
>Mature_214_residues
AIQILLVDDQYLIREGIASLLELEEDLEVAGMAANGKEAIKQAIDLKPNIILMDIRMPELNGVEATAKIKQSLPHCQIIM
LTTFDDEELIVQSLLAGACGYLMKDIPPDDLGQAIKLADAGVFQLAPEVAGKLIGSLKTQRAGDAPKLPTDTPLTTRELE
VLKLLAKGNTNKEIAKAFNLSEGTVKNHVSNILMRLGLRDRTQAAVYAVEHGLS

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787473, Length=204, Percent_Identity=37.7450980392157, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1788521, Length=210, Percent_Identity=34.2857142857143, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=32.5471698113208, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790102, Length=204, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=27.1844660194175, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788712, Length=192, Percent_Identity=25.5208333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=116, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082052, Length=106, Percent_Identity=33.0188679245283, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788546, Length=202, Percent_Identity=26.2376237623762, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23278; Mature: 23147

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQILLVDDQYLIREGIASLLELEEDLEVAGMAANGKEAIKQAIDLKPNIILMDIRMPE
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCC
LNGVEATAKIKQSLPHCQIIMLTTFDDEELIVQSLLAGACGYLMKDIPPDDLGQAIKLAD
CCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
AGVFQLAPEVAGKLIGSLKTQRAGDAPKLPTDTPLTTRELEVLKLLAKGNTNKEIAKAFN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHC
LSEGTVKNHVSNILMRLGLRDRTQAAVYAVEHGLS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AIQILLVDDQYLIREGIASLLELEEDLEVAGMAANGKEAIKQAIDLKPNIILMDIRMPE
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCC
LNGVEATAKIKQSLPHCQIIMLTTFDDEELIVQSLLAGACGYLMKDIPPDDLGQAIKLAD
CCCCHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
AGVFQLAPEVAGKLIGSLKTQRAGDAPKLPTDTPLTTRELEVLKLLAKGNTNKEIAKAFN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHC
LSEGTVKNHVSNILMRLGLRDRTQAAVYAVEHGLS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]