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The map label for this gene is pleD [H]

Identifier: 158340437

GI number: 158340437

Start: 249694

End: 250926

Strand: Direct

Name: pleD [H]

Synonym: AM1_C0365

Alternate gene names: 158340437

Gene position: 249694-250926 (Clockwise)

Preceding gene: 158340435

Following gene: 158340438

Centisome position: 91.42

GC content: 49.07

Gene sequence:

>1233_bases
GTGGTGGATGACCAGCCTAATATCATGAACCTACTCACCAGCATTATGGAACAGGATGAGTATGAGGTCTGGCAGGCCAG
CAGTGGGGCGGAGGGGTTGCAAATGGCCGCGAAGGTGCTGCCGGATTTGATTTTGCTGGATGTCAACATGCCTGATATGG
ATGGTTATACCGTCTGCCAGCAGCTCAAGGCAGACCCACAGACTACCGCCATTCCCATTATTTTTGTCAGCGCCCTGGAT
GAAACCTGGGATAAGGTGAAAGGGTTTTCCGTCGGTGGCAGTGACTACATTACCAAACCCTTTAACACCCTAGAGGTGAT
TGTTCGCATTGAGGATCAACTGAAAATTCGCCAGATCCAAGACCAAATGCTGAAGGCGGATCAAGCGCCAACTGATTCTC
CTCAATCGAAGCCATCTTCCGCCAAAGCCGTCATTCTAATGGCGGATGATGAACCCAAGAACTTAGCCCTGCTGACGGAT
GTATTAGAGCAGGAAGGGTATGAAATCTGGCAGGCGGATAGTGGTGCGGAGGCGTTAAGGGTGGCCGGCATGGTGCTACC
GGATCTGATTCTGCTGGATATCAATATGCCCGATATGCAAGGCTACGATGTGTGTCAACAACTGAAAGCAAACGCTAAAA
CCAAACTAATTCCGGTGATTTTTGTCAGCGCCCTGGATGAAACCTGGGATAAGGTGAAGGCATTTTCTGTGGGAGGCAGT
GACTACATCACCAAACCCTTTAACACCCTAGAGGTGATTGTTCGCATTGAGAATCAACTGGAAATTCGCCAGATCCAAGA
CCAAATGCTGAAGGCGGATCAAGCGCCAACTGATTCTCCTCAACCGACGCCATCTTCCGCCAAAGCCGTCATTCTGATGG
TGGATGATGAACCTAAGAATTTAGCCCTGCTGACGGATGTATTAGAGCATGATGGGTATGAAATCTGGCAGGCGGATAGT
GGTTCGGAGGCGTTAAGGGTGGCCGGCATGGTGCTACCGGATCTGATTCTGCTGGATATCAATATGCCCGATATGCAAGG
CTACGATGTGTGTCAACAACTGAAAGCAAACGCTAAAACCAAACTAATTCCGGTGATTTTTGTCAGCGCCCTGGATGAAA
CCTGGGATAAGGTGAAGGCATTTTCTGTGGGGGGCAGTGATTACATTAATAAGCCGATTCAGATCGTTGAATTAATGGCT
CGGATGAAGCATCAACTGCAAATTTGGCACTAA

Upstream 100 bases:

>100_bases
GGCTAAAGCGACTGAAATTAAGGAGACTAACGGTAACAAGACTGACGTTAATCAAGCCGAAGTTAAGGAGAAGGCGACGG
TTATACCATCCGTTATTCTG

Downstream 100 bases:

>100_bases
AAATTTAACGAGATGACGATGCAACGCCACTCAGCCAGGGATCTCAAACTGACAAAACCGATGGCAAATTTAGCATGAAG
GAATCATCAGATGAACCCCA

Product: response regulator receiver, CheY-like protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MVDDQPNIMNLLTSIMEQDEYEVWQASSGAEGLQMAAKVLPDLILLDVNMPDMDGYTVCQQLKADPQTTAIPIIFVSALD
ETWDKVKGFSVGGSDYITKPFNTLEVIVRIEDQLKIRQIQDQMLKADQAPTDSPQSKPSSAKAVILMADDEPKNLALLTD
VLEQEGYEIWQADSGAEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGS
DYITKPFNTLEVIVRIENQLEIRQIQDQMLKADQAPTDSPQPTPSSAKAVILMVDDEPKNLALLTDVLEHDGYEIWQADS
GSEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGSDYINKPIQIVELMA
RMKHQLQIWH

Sequences:

>Translated_410_residues
MVDDQPNIMNLLTSIMEQDEYEVWQASSGAEGLQMAAKVLPDLILLDVNMPDMDGYTVCQQLKADPQTTAIPIIFVSALD
ETWDKVKGFSVGGSDYITKPFNTLEVIVRIEDQLKIRQIQDQMLKADQAPTDSPQSKPSSAKAVILMADDEPKNLALLTD
VLEQEGYEIWQADSGAEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGS
DYITKPFNTLEVIVRIENQLEIRQIQDQMLKADQAPTDSPQPTPSSAKAVILMVDDEPKNLALLTDVLEHDGYEIWQADS
GSEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGSDYINKPIQIVELMA
RMKHQLQIWH
>Mature_410_residues
MVDDQPNIMNLLTSIMEQDEYEVWQASSGAEGLQMAAKVLPDLILLDVNMPDMDGYTVCQQLKADPQTTAIPIIFVSALD
ETWDKVKGFSVGGSDYITKPFNTLEVIVRIEDQLKIRQIQDQMLKADQAPTDSPQSKPSSAKAVILMADDEPKNLALLTD
VLEQEGYEIWQADSGAEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGS
DYITKPFNTLEVIVRIENQLEIRQIQDQMLKADQAPTDSPQPTPSSAKAVILMVDDEPKNLALLTDVLEHDGYEIWQADS
GSEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGSDYINKPIQIVELMA
RMKHQLQIWH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=33.9130434782609, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=32.4561403508772, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=32.2834645669291, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=35.6521739130435, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=27.4074074074074, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082012, Length=158, Percent_Identity=29.746835443038, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=36.283185840708, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=171, Percent_Identity=29.2397660818713, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787229, Length=116, Percent_Identity=33.6206896551724, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789809, Length=145, Percent_Identity=30.3448275862069, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=37.2727272727273, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI145693157, Length=129, Percent_Identity=36.4341085271318, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45563; Mature: 45563

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDDQPNIMNLLTSIMEQDEYEVWQASSGAEGLQMAAKVLPDLILLDVNMPDMDGYTVCQ
CCCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
QLKADPQTTAIPIIFVSALDETWDKVKGFSVGGSDYITKPFNTLEVIVRIEDQLKIRQIQ
HHCCCCCCCEEHEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
DQMLKADQAPTDSPQSKPSSAKAVILMADDEPKNLALLTDVLEQEGYEIWQADSGAEALR
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
VAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGS
HHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEEEECCCC
DYITKPFNTLEVIVRIENQLEIRQIQDQMLKADQAPTDSPQPTPSSAKAVILMVDDEPKN
CCCCCCHHHHHEEHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCC
LALLTDVLEHDGYEIWQADSGSEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKT
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCC
KLIPVIFVSALDETWDKVKAFSVGGSDYINKPIQIVELMARMKHQLQIWH
EEHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVDDQPNIMNLLTSIMEQDEYEVWQASSGAEGLQMAAKVLPDLILLDVNMPDMDGYTVCQ
CCCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHH
QLKADPQTTAIPIIFVSALDETWDKVKGFSVGGSDYITKPFNTLEVIVRIEDQLKIRQIQ
HHCCCCCCCEEHEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
DQMLKADQAPTDSPQSKPSSAKAVILMADDEPKNLALLTDVLEQEGYEIWQADSGAEALR
HHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCHHHHH
VAGMVLPDLILLDINMPDMQGYDVCQQLKANAKTKLIPVIFVSALDETWDKVKAFSVGGS
HHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHEEEECCCC
DYITKPFNTLEVIVRIENQLEIRQIQDQMLKADQAPTDSPQPTPSSAKAVILMVDDEPKN
CCCCCCHHHHHEEHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCC
LALLTDVLEHDGYEIWQADSGSEALRVAGMVLPDLILLDINMPDMQGYDVCQQLKANAKT
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCCC
KLIPVIFVSALDETWDKVKAFSVGGSDYINKPIQIVELMARMKHQLQIWH
EEHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA