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The map label for this gene is rpoD [H]

Identifier: 158340427

GI number: 158340427

Start: 243801

End: 244622

Strand: Direct

Name: rpoD [H]

Synonym: AM1_C0355

Alternate gene names: 158340427

Gene position: 243801-244622 (Clockwise)

Preceding gene: 158340425

Following gene: 158340428

Centisome position: 89.26

GC content: 47.69

Gene sequence:

>822_bases
ATGATTCAAACTGACAGCACTGGCGCTTATCTCAAAGAAATCGGACGCTATCCCCTTCTATCCCACGAAGAAGAAATCCA
ACTTGCCAGACAAGCTAAAGCTGGAAACCTTCGGGCCAAACAACGGATGATCGAGTGCAATCTGCGCCTGGTCGTCTCCA
TTGCTAAAAAGCATCAGAATCGGGGCTTGCCATTCATGGACTTAATCCAAGAAGGCAGCATCGGGCTGAGTCGAGCCGTT
GAGAAGTTCGAGCCTGAGCAGGGGTATCGCTTCAGTACCTATGCCTATTGGTGGATTCGACAGGGAATAACCCGCTCGAT
ACAGAATAACAGTCGTGTGATTCGCCTCCCTGTGTGGCATTGGCAGACAGGAAACCAAATCAAACAGACTCAACGACAAC
TCTCCCAAGAATTCGGTCGTGAGCCTACACTTGCTGAACTTGCTGAGGCAATGGAGATTGACCTCGACAAACTGAAGCAA
AACATGCATCATCTTCAAGATGTTCTCTCTCTCGATATGCAGGTTGGCAAGGAACAAGACACCACACTAGGAGAACTGAT
CGAAGCAGAACAATCGACACCAACCCATCTTGAACTGCTGAACCAGAGTGAAGAGGTATCGAGTTATTTATCACTCCTAG
ATGAACGGCAACGCTATGTGATTGTCCAGCGATATGGATTAGAAGATGGCGAAGCTAAGACCATGCAAGATATTGGTCAG
CAGCTTGGCGTCAGCCGGGAGCGGATTCGACAAATTATCAACAAAGCGATGAAGAAATTACAGAAAAACAGTGACCTAGC
TACAGAAATCAAGACGGCCTAG

Upstream 100 bases:

>100_bases
TTACTTCCTCTGAGTTTCTTAAGAAATAACACAAGACCATTCCCATCTCTGGCATTTCAGGGTATTCCTCTACCAACGAT
GTTTGCAAGGCATCAAGCGG

Downstream 100 bases:

>100_bases
TCCTCGCCAGGACCTAGGATAGAAAGAGCCCCCTAATGCTCATCAGCAGATCCAGGGGGCATACACCAGAATCATTAGGG
TTCTCTGAAAACCCCACTCA

Product: RNA polymerase sigma-70 factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIQTDSTGAYLKEIGRYPLLSHEEEIQLARQAKAGNLRAKQRMIECNLRLVVSIAKKHQNRGLPFMDLIQEGSIGLSRAV
EKFEPEQGYRFSTYAYWWIRQGITRSIQNNSRVIRLPVWHWQTGNQIKQTQRQLSQEFGREPTLAELAEAMEIDLDKLKQ
NMHHLQDVLSLDMQVGKEQDTTLGELIEAEQSTPTHLELLNQSEEVSSYLSLLDERQRYVIVQRYGLEDGEAKTMQDIGQ
QLGVSRERIRQIINKAMKKLQKNSDLATEIKTA

Sequences:

>Translated_273_residues
MIQTDSTGAYLKEIGRYPLLSHEEEIQLARQAKAGNLRAKQRMIECNLRLVVSIAKKHQNRGLPFMDLIQEGSIGLSRAV
EKFEPEQGYRFSTYAYWWIRQGITRSIQNNSRVIRLPVWHWQTGNQIKQTQRQLSQEFGREPTLAELAEAMEIDLDKLKQ
NMHHLQDVLSLDMQVGKEQDTTLGELIEAEQSTPTHLELLNQSEEVSSYLSLLDERQRYVIVQRYGLEDGEAKTMQDIGQ
QLGVSRERIRQIINKAMKKLQKNSDLATEIKTA
>Mature_273_residues
MIQTDSTGAYLKEIGRYPLLSHEEEIQLARQAKAGNLRAKQRMIECNLRLVVSIAKKHQNRGLPFMDLIQEGSIGLSRAV
EKFEPEQGYRFSTYAYWWIRQGITRSIQNNSRVIRLPVWHWQTGNQIKQTQRQLSQEFGREPTLAELAEAMEIDLDKLKQ
NMHHLQDVLSLDMQVGKEQDTTLGELIEAEQSTPTHLELLNQSEEVSSYLSLLDERQRYVIVQRYGLEDGEAKTMQDIGQ
QLGVSRERIRQIINKAMKKLQKNSDLATEIKTA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=41.6030534351145, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=46.5811965811966, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1789871, Length=278, Percent_Identity=29.4964028776978, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788231, Length=198, Percent_Identity=28.2828282828283, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31549; Mature: 31549

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTDSTGAYLKEIGRYPLLSHEEEIQLARQAKAGNLRAKQRMIECNLRLVVSIAKKHQN
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
RGLPFMDLIQEGSIGLSRAVEKFEPEQGYRFSTYAYWWIRQGITRSIQNNSRVIRLPVWH
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
WQTGNQIKQTQRQLSQEFGREPTLAELAEAMEIDLDKLKQNMHHLQDVLSLDMQVGKEQD
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TTLGELIEAEQSTPTHLELLNQSEEVSSYLSLLDERQRYVIVQRYGLEDGEAKTMQDIGQ
HHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHHHHHHH
QLGVSRERIRQIINKAMKKLQKNSDLATEIKTA
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MIQTDSTGAYLKEIGRYPLLSHEEEIQLARQAKAGNLRAKQRMIECNLRLVVSIAKKHQN
CCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
RGLPFMDLIQEGSIGLSRAVEKFEPEQGYRFSTYAYWWIRQGITRSIQNNSRVIRLPVWH
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
WQTGNQIKQTQRQLSQEFGREPTLAELAEAMEIDLDKLKQNMHHLQDVLSLDMQVGKEQD
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TTLGELIEAEQSTPTHLELLNQSEEVSSYLSLLDERQRYVIVQRYGLEDGEAKTMQDIGQ
HHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHHHHHHH
QLGVSRERIRQIINKAMKKLQKNSDLATEIKTA
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA