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The map label for this gene is hup [H]

Identifier: 158340425

GI number: 158340425

Start: 243339

End: 243614

Strand: Direct

Name: hup [H]

Synonym: AM1_C0353

Alternate gene names: 158340425

Gene position: 243339-243614 (Clockwise)

Preceding gene: 158340424

Following gene: 158340427

Centisome position: 89.1

GC content: 44.93

Gene sequence:

>276_bases
ATGAATAAAGGCGAACTCGTTGACGCAATTGCTGAAAATACTGGCGTCACTAAGAAGCAAGCTGATACTACCCTTTCGGC
TACTATTGAGACAATCGTTGATGCTGTTAGCTCTGGTGAAAAAGTTACTCTCGTTGGCTTCGGCAGTTTTGAAGCTAGAG
ACCGCAAAGCTAGAGAGGGACGTAATCCTAAGACGGGTGAGAAAATGACTACACCAGCTTCCAAAGCACCTGTTTTCAGC
GCTGGCAAGCTTTTCAAGGAAAAAGTAAAACCCTGA

Upstream 100 bases:

>100_bases
CAATTGATATTCCCAGACCCAGTTATATTGCCCAGGTTCCCAGATACCCCCTATTACAATCCTTGCAGTACAATACTCGC
AATCCTTTCAAGGTACTGTC

Downstream 100 bases:

>100_bases
TAGACTGAAGCATATCTATCATTCTATTTTCTACAATCTAGCTGGCAGATCTTCTTGTGATTCTGCCAGCTCTTTATTAG
GTGCGATTACTTCCTCTGAG

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MNKGELVDAIAENTGVTKKQADTTLSATIETIVDAVSSGEKVTLVGFGSFEARDRKAREGRNPKTGEKMTTPASKAPVFS
AGKLFKEKVKP

Sequences:

>Translated_91_residues
MNKGELVDAIAENTGVTKKQADTTLSATIETIVDAVSSGEKVTLVGFGSFEARDRKAREGRNPKTGEKMTTPASKAPVFS
AGKLFKEKVKP
>Mature_91_residues
MNKGELVDAIAENTGVTKKQADTTLSATIETIVDAVSSGEKVTLVGFGSFEARDRKAREGRNPKTGEKMTTPASKAPVFS
AGKLFKEKVKP

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. It is essential for heterocyst differentiation [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=43.8202247191011, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=42.2222222222222, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=3e-13,
Organism=Escherichia coli, GI1787141, Length=89, Percent_Identity=31.4606741573034, Blast_Score=59, Evalue=5e-11,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9670; Mature: 9670

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGELVDAIAENTGVTKKQADTTLSATIETIVDAVSSGEKVTLVGFGSFEARDRKAREG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCC
RNPKTGEKMTTPASKAPVFSAGKLFKEKVKP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNKGELVDAIAENTGVTKKQADTTLSATIETIVDAVSSGEKVTLVGFGSFEARDRKAREG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHCC
RNPKTGEKMTTPASKAPVFSAGKLFKEKVKP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655556; 7748931; 11759840 [H]