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The map label for this gene is ltrA [H]

Identifier: 158340400

GI number: 158340400

Start: 229079

End: 230377

Strand: Reverse

Name: ltrA [H]

Synonym: AM1_C0328

Alternate gene names: 158340400

Gene position: 230377-229079 (Counterclockwise)

Preceding gene: 158340406

Following gene: 158340397

Centisome position: 84.35

GC content: 48.65

Gene sequence:

>1299_bases
ATGGCGGAAACAGGAGTTGAACGGATAGCTAGTCGGGCTCGTAATCATCCAGAGGAGCCATTCACGGCTCTGATGCACCA
CTATAGTGTGGAAAATCTGCGAGCCTGCTTCGAGTCGCTGGATGGAAACAAAGCGCTTGGAGTAGATGGAGTGACCAAGG
CGGAGTACCAGGAAAATTTGGAGACTAATCTCCAGAATCTACACCTGAAACTACGCCAAATGTCGTATCGACCCCAACCG
GTGCGTCAGGTAGAGATCCCAAAAGAGGATGGCAGTATGCGTCCTTTAGGGATCAGCTGTACGGAAGATAAAGTAGTTCA
GGAGATGACCCGTCGGATCCTAGAAGCGATCTATGAACCCGTGTTTATCGACACCTCATATGGATTCCGCCCAAAGCGGA
GCTGCCATGATGCTTTAAGGCAGCTAAATAGGGAAGTGATGCGTAAACCCGTAAATTGGGTCGCTGATATCGATCTAGCC
AAATTCTTCGACACAATGCCTCACCAGGAAATCCTTTCCGTATTGAGTATCCGAATAAAGGACGGGAATCTTCTGCGTCT
GATAGCACGGATGCTCAAAGCGGGCATACAAACACCTGGAGGCGTGGTGTACGACGAACTCGGAAGTCCTCAGGGCTCAA
TCGTCTCCCCTGTGATTGCGAATATCTTCCTCGACTACGTGTTGGACCAATGGTTTACCAACGTAGTGAGACATCACTGC
CGAGGGTATTGCGCCATTATTCGATACGCAGACGATGTTGCGGCTGTGTTTGAACATGAAGAAGATGCCATTAGATTTAT
GCGGGTACTGCCGCGACGGCTGGAGAAATATGGCTTACGACTAAATACAAAGAAAACCCACTTGTTGGCCTTTGGTAAAA
GAAATGCTCGGCGCTGTTTCCAGACTGGGCAACGTCCATCAACCTTTGATTTTCTTGGGTTGACCCACTATTGGGGCCGG
AGTCGCAAGGGGTATGTCCGCATGAAACGTAAGACATCAAAGAAACGGCTACGTCGCTCCCTTAAGCAACTAAAAATGTG
GTTGCGGAAGGTACGCAATATCTATAAGCTTCCTGTGATCTGGCGAGTAGTGAGTTTGAAGTTGTGTGGGCACTTTAACT
ACTTTGGAGTGACCGACAATAGTCAATCTCTGTACTACTTCAGGCGAGAAGTCTACAAGCTGCTATTTAAGTGGCTCAAT
CGTCGCAGTCAACGGCGAAGCTTTTCATGGGGTGATTTTCTGCACTATGAAGCATGCTACCCATTACCACGCCCCAGGCG
ATTGGTTTCTCTCTATTGA

Upstream 100 bases:

>100_bases
CTCATAGTAGTGAAGAAATGGAGTAACGCCCATGGAGCGAAGGGGGACACATATAGTCGCCCGTCCAACGGAAACGTCAT
CTACACGCAGAGGTGGACTC

Downstream 100 bases:

>100_bases
ACCTGACAAAGGCTATATGAAGAGGCTCGTGCGGGAAAACCGCAGGCGGGCTTCTGTGGGGGGGAGGCTCTCAACAATAT
AGGGTCAAATCTTGTGACAC

Product: RNA-directed DNA polymerase

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MAETGVERIASRARNHPEEPFTALMHHYSVENLRACFESLDGNKALGVDGVTKAEYQENLETNLQNLHLKLRQMSYRPQP
VRQVEIPKEDGSMRPLGISCTEDKVVQEMTRRILEAIYEPVFIDTSYGFRPKRSCHDALRQLNREVMRKPVNWVADIDLA
KFFDTMPHQEILSVLSIRIKDGNLLRLIARMLKAGIQTPGGVVYDELGSPQGSIVSPVIANIFLDYVLDQWFTNVVRHHC
RGYCAIIRYADDVAAVFEHEEDAIRFMRVLPRRLEKYGLRLNTKKTHLLAFGKRNARRCFQTGQRPSTFDFLGLTHYWGR
SRKGYVRMKRKTSKKRLRRSLKQLKMWLRKVRNIYKLPVIWRVVSLKLCGHFNYFGVTDNSQSLYYFRREVYKLLFKWLN
RRSQRRSFSWGDFLHYEACYPLPRPRRLVSLY

Sequences:

>Translated_432_residues
MAETGVERIASRARNHPEEPFTALMHHYSVENLRACFESLDGNKALGVDGVTKAEYQENLETNLQNLHLKLRQMSYRPQP
VRQVEIPKEDGSMRPLGISCTEDKVVQEMTRRILEAIYEPVFIDTSYGFRPKRSCHDALRQLNREVMRKPVNWVADIDLA
KFFDTMPHQEILSVLSIRIKDGNLLRLIARMLKAGIQTPGGVVYDELGSPQGSIVSPVIANIFLDYVLDQWFTNVVRHHC
RGYCAIIRYADDVAAVFEHEEDAIRFMRVLPRRLEKYGLRLNTKKTHLLAFGKRNARRCFQTGQRPSTFDFLGLTHYWGR
SRKGYVRMKRKTSKKRLRRSLKQLKMWLRKVRNIYKLPVIWRVVSLKLCGHFNYFGVTDNSQSLYYFRREVYKLLFKWLN
RRSQRRSFSWGDFLHYEACYPLPRPRRLVSLY
>Mature_431_residues
AETGVERIASRARNHPEEPFTALMHHYSVENLRACFESLDGNKALGVDGVTKAEYQENLETNLQNLHLKLRQMSYRPQPV
RQVEIPKEDGSMRPLGISCTEDKVVQEMTRRILEAIYEPVFIDTSYGFRPKRSCHDALRQLNREVMRKPVNWVADIDLAK
FFDTMPHQEILSVLSIRIKDGNLLRLIARMLKAGIQTPGGVVYDELGSPQGSIVSPVIANIFLDYVLDQWFTNVVRHHCR
GYCAIIRYADDVAAVFEHEEDAIRFMRVLPRRLEKYGLRLNTKKTHLLAFGKRNARRCFQTGQRPSTFDFLGLTHYWGRS
RKGYVRMKRKTSKKRLRRSLKQLKMWLRKVRNIYKLPVIWRVVSLKLCGHFNYFGVTDNSQSLYYFRREVYKLLFKWLNR
RSQRRSFSWGDFLHYEACYPLPRPRRLVSLY

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=197, Percent_Identity=37.0558375634518, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6226520, Length=180, Percent_Identity=35.5555555555556, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 50913; Mature: 50782

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETGVERIASRARNHPEEPFTALMHHYSVENLRACFESLDGNKALGVDGVTKAEYQENL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
ETNLQNLHLKLRQMSYRPQPVRQVEIPKEDGSMRPLGISCTEDKVVQEMTRRILEAIYEP
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
VFIDTSYGFRPKRSCHDALRQLNREVMRKPVNWVADIDLAKFFDTMPHQEILSVLSIRIK
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DGNLLRLIARMLKAGIQTPGGVVYDELGSPQGSIVSPVIANIFLDYVLDQWFTNVVRHHC
CCHHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGYCAIIRYADDVAAVFEHEEDAIRFMRVLPRRLEKYGLRLNTKKTHLLAFGKRNARRCF
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCHHHHHHH
QTGQRPSTFDFLGLTHYWGRSRKGYVRMKRKTSKKRLRRSLKQLKMWLRKVRNIYKLPVI
HCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRVVSLKLCGHFNYFGVTDNSQSLYYFRREVYKLLFKWLNRRSQRRSFSWGDFLHYEACY
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
PLPRPRRLVSLY
CCCCCCHHHCCC
>Mature Secondary Structure 
AETGVERIASRARNHPEEPFTALMHHYSVENLRACFESLDGNKALGVDGVTKAEYQENL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHH
ETNLQNLHLKLRQMSYRPQPVRQVEIPKEDGSMRPLGISCTEDKVVQEMTRRILEAIYEP
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
VFIDTSYGFRPKRSCHDALRQLNREVMRKPVNWVADIDLAKFFDTMPHQEILSVLSIRIK
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DGNLLRLIARMLKAGIQTPGGVVYDELGSPQGSIVSPVIANIFLDYVLDQWFTNVVRHHC
CCHHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGYCAIIRYADDVAAVFEHEEDAIRFMRVLPRRLEKYGLRLNTKKTHLLAFGKRNARRCF
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCHHHHHHH
QTGQRPSTFDFLGLTHYWGRSRKGYVRMKRKTSKKRLRRSLKQLKMWLRKVRNIYKLPVI
HCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRVVSLKLCGHFNYFGVTDNSQSLYYFRREVYKLLFKWLNRRSQRRSFSWGDFLHYEACY
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCC
PLPRPRRLVSLY
CCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]