Click here to switch to the map view.

The map label for this gene is moeZ [H]

Identifier: 158340346

GI number: 158340346

Start: 193489

End: 194769

Strand: Reverse

Name: moeZ [H]

Synonym: AM1_C0271

Alternate gene names: 158340346

Gene position: 194769-193489 (Counterclockwise)

Preceding gene: 158340347

Following gene: 158340345

Centisome position: 71.31

GC content: 32.71

Gene sequence:

>1281_bases
ATGGAATTACCTTTAATATATATATTTAATGATGAAATAAGTGAAATCTTTAATAAGCTAAAGAACTCAAAATCTGATTG
TCTCAATCTATTTTCTTACTGTTTGGATGAAGGAGAAGTCTATCATATTTATAGTGATTTACCTAAGATTCACTTCTCAG
GTACCCCAAAAAGAGCTGTTGCAAGAATTTTTGATGATATTTCTGATAAAGAAAAGTGTATAAAATTTGCTAAAAAAACT
TACCGGGATACTGACATTAATGACCATGATGATTCAAGAATCATTATATTATTCGCTAATGAACAGGCATTTAGAGTCTT
TATAGTAATTGAAGCAGAAATTTTTGAAGGAATTATAAAAATTGTGCCTGGAATTTCAGAAATTTACTCTCGTAGTAAAG
GTCTCTTGGAGACCTCGATACTTAATGCTTCTAATGTAGGATTAATTGGGTTAGGGAGTGGAGGATCATCAGTAGCGATA
GAGTTAGCTAAAGCAGGCGTTGGAAAATTTATTTTAGTTGATTTTGATAGACTAGAATTATCAAATATATCGAGACATAT
TTGTGGTATTAATGATTTAGGGCGATTCAAAACTAAAGCAATTAAAGATTTACTGTTAGCTAAAAATCCATACATTGAAG
TTGAGACCTATGAGGTTGATATTAATACTGATTTGTCTAAAGTTAGTAAAGAACTTAAACAATGCAACTTAATTATTGGT
GCAACTGATAATAACAGAAGTCGATTTAATATTAATCAGTTTGCCTTGGATCATTCTATAACAACTATTTTTGGAAGAGC
AATTACTAGAGCCGTAGGTGGGGATGTTTTAAGAGTTCGTCCAAGACTTGGCCCATGTTTAGCATGTGTGTTTACGAAGG
AATTCGCCAATAGCTTTCCAGATGAAGTTTCTCATTTTAAGCAAGCCCGCAATGAAATGCCTGACTATGTCTCAGATGAC
AGGGTTGAAGCAACGATACAAGTTGGGTTATCTTCCGACATTCAACCTATTTCTAATATGATTGTTAAGCTTGCTTTGTT
AGAATTATCTAAAAACAAAGAAAGTGGATTAACTTCACTTGAAAAAGACCTAATAGCAGATTTTTATATTTGGGCAAATC
GCCGAGAACTTGCTTATTCAAACTATCAGATAATGGAATATGGTTCTCAAAAGCCCTCAATTTTACGTTGGTATGGCGCT
AAAATTGAACGCAATCCCTATTGTCCTGTATGTGCTGAGATAGAAGAATCTGACTTAAGCGATGACAATATTTTTAAATG
A

Upstream 100 bases:

>100_bases
AAAAAACTTCAGGCTCAATGATTTTAGATATTTATTTAGATTTTTGAAATCAAATTAGATCCAATTAGTTTTTTAAAATT
TTCGTTTTGAGAATATTATA

Downstream 100 bases:

>100_bases
AATACATGAATTATAATTTATAAGCTAATAACATAATTCCTCTTCTCAATATAAGCTTATTCTTTTTCTTCATTTATCAT
CTTCACCTCAGAGTCTACGA

Product: ThiF family protein, putative

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MELPLIYIFNDEISEIFNKLKNSKSDCLNLFSYCLDEGEVYHIYSDLPKIHFSGTPKRAVARIFDDISDKEKCIKFAKKT
YRDTDINDHDDSRIIILFANEQAFRVFIVIEAEIFEGIIKIVPGISEIYSRSKGLLETSILNASNVGLIGLGSGGSSVAI
ELAKAGVGKFILVDFDRLELSNISRHICGINDLGRFKTKAIKDLLLAKNPYIEVETYEVDINTDLSKVSKELKQCNLIIG
ATDNNRSRFNINQFALDHSITTIFGRAITRAVGGDVLRVRPRLGPCLACVFTKEFANSFPDEVSHFKQARNEMPDYVSDD
RVEATIQVGLSSDIQPISNMIVKLALLELSKNKESGLTSLEKDLIADFYIWANRRELAYSNYQIMEYGSQKPSILRWYGA
KIERNPYCPVCAEIEESDLSDDNIFK

Sequences:

>Translated_426_residues
MELPLIYIFNDEISEIFNKLKNSKSDCLNLFSYCLDEGEVYHIYSDLPKIHFSGTPKRAVARIFDDISDKEKCIKFAKKT
YRDTDINDHDDSRIIILFANEQAFRVFIVIEAEIFEGIIKIVPGISEIYSRSKGLLETSILNASNVGLIGLGSGGSSVAI
ELAKAGVGKFILVDFDRLELSNISRHICGINDLGRFKTKAIKDLLLAKNPYIEVETYEVDINTDLSKVSKELKQCNLIIG
ATDNNRSRFNINQFALDHSITTIFGRAITRAVGGDVLRVRPRLGPCLACVFTKEFANSFPDEVSHFKQARNEMPDYVSDD
RVEATIQVGLSSDIQPISNMIVKLALLELSKNKESGLTSLEKDLIADFYIWANRRELAYSNYQIMEYGSQKPSILRWYGA
KIERNPYCPVCAEIEESDLSDDNIFK
>Mature_426_residues
MELPLIYIFNDEISEIFNKLKNSKSDCLNLFSYCLDEGEVYHIYSDLPKIHFSGTPKRAVARIFDDISDKEKCIKFAKKT
YRDTDINDHDDSRIIILFANEQAFRVFIVIEAEIFEGIIKIVPGISEIYSRSKGLLETSILNASNVGLIGLGSGGSSVAI
ELAKAGVGKFILVDFDRLELSNISRHICGINDLGRFKTKAIKDLLLAKNPYIEVETYEVDINTDLSKVSKELKQCNLIIG
ATDNNRSRFNINQFALDHSITTIFGRAITRAVGGDVLRVRPRLGPCLACVFTKEFANSFPDEVSHFKQARNEMPDYVSDD
RVEATIQVGLSSDIQPISNMIVKLALLELSKNKESGLTSLEKDLIADFYIWANRRELAYSNYQIMEYGSQKPSILRWYGA
KIERNPYCPVCAEIEESDLSDDNIFK

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 48080; Mature: 48080

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPLIYIFNDEISEIFNKLKNSKSDCLNLFSYCLDEGEVYHIYSDLPKIHFSGTPKRAV
CCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHH
ARIFDDISDKEKCIKFAKKTYRDTDINDHDDSRIIILFANEQAFRVFIVIEAEIFEGIIK
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEHHHHHHHHH
IVPGISEIYSRSKGLLETSILNASNVGLIGLGSGGSSVAIELAKAGVGKFILVDFDRLEL
HCCCHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEECHHHH
SNISRHICGINDLGRFKTKAIKDLLLAKNPYIEVETYEVDINTDLSKVSKELKQCNLIIG
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHCEEEEE
ATDNNRSRFNINQFALDHSITTIFGRAITRAVGGDVLRVRPRLGPCLACVFTKEFANSFP
ECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCH
DEVSHFKQARNEMPDYVSDDRVEATIQVGLSSDIQPISNMIVKLALLELSKNKESGLTSL
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
EKDLIADFYIWANRRELAYSNYQIMEYGSQKPSILRWYGAKIERNPYCPVCAEIEESDLS
HHHHHHHHHEEECCHHHEECCEEEEECCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCC
DDNIFK
CCCCCC
>Mature Secondary Structure
MELPLIYIFNDEISEIFNKLKNSKSDCLNLFSYCLDEGEVYHIYSDLPKIHFSGTPKRAV
CCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHH
ARIFDDISDKEKCIKFAKKTYRDTDINDHDDSRIIILFANEQAFRVFIVIEAEIFEGIIK
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEEEEEHHHHHHHHH
IVPGISEIYSRSKGLLETSILNASNVGLIGLGSGGSSVAIELAKAGVGKFILVDFDRLEL
HCCCHHHHHHCCCCCHHHHHCCCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEECHHHH
SNISRHICGINDLGRFKTKAIKDLLLAKNPYIEVETYEVDINTDLSKVSKELKQCNLIIG
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHCEEEEE
ATDNNRSRFNINQFALDHSITTIFGRAITRAVGGDVLRVRPRLGPCLACVFTKEFANSFP
ECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCH
DEVSHFKQARNEMPDYVSDDRVEATIQVGLSSDIQPISNMIVKLALLELSKNKESGLTSL
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
EKDLIADFYIWANRRELAYSNYQIMEYGSQKPSILRWYGAKIERNPYCPVCAEIEESDLS
HHHHHHHHHEEECCHHHEECCEEEEECCCCCCCEEEEECCCCCCCCCCCEECCCCCCCCC
DDNIFK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]