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The map label for this gene is phaZ1 [H]

Identifier: 148243673

GI number: 148243673

Start: 50396

End: 51322

Strand: Reverse

Name: phaZ1 [H]

Synonym: Acry_3146

Alternate gene names: 148243673

Gene position: 51322-50396 (Counterclockwise)

Preceding gene: 148243674

Following gene: 148243672

Centisome position: 25.21

GC content: 68.72

Gene sequence:

>927_bases
ATGTCGGCAGGTTGCGTGTCGGAAGTCGTCGGGTTCGGCTCCAATCCCGGCGCATTGAGGATGCTGGTTCATGCCCGCGC
CCGGCTGCCCGCGGGCCGGCCGCTGGTCGTCGTCCTGCACGGCTGCGGCCAGGATGCCGCAGCCTTTGCCAACGCGTCCG
GCTGGCTCGCGCTGGCGGACGAGTTGGGGTTCGCGCTGCTGGTGCCCGAACAGGTTCACGCGAACAACCGTGGCCGCTGC
TTCAACTGGTTCCGCCCTGATGACGTCCGCCGGAGCGGCGGCGAGGCGATGTCGATTCGCCAGATGCTGAGAGTCGCGGT
CGCACGCTATGGGTCCGACCCAAGACGAATCTACATTGTCGGATTTTCTGCCGGCGGCGGCATGGCCGCTGCGATGCTCG
CCGCATACCCTGCGGTGTTCGCTGCGGGGGGCATTGTCGCGGGCATGCCGGTCGGCTGCGCGCAAACCCAGGTCGCAGCG
ATGCTTCACATGCGACGGGCAAACATGTCGCGGTCGCGGCAGGCGCTCGCTGGCGACGTCCGTGCAGTGACGCAGGCCCG
TTCACGACGGTCGTGGCCACGCGTCTCGATCTGGCAAGGCGGCCGAGATCGCACGGTTGATCCGGCGAACGCCGAAATAC
TCGCGGCGCAATGGGGCGAGTTGCATGGCCAGGATACGGCGCCGAGGACCGACAACGTGGCATCGGGGCATCGGCATCGG
ACCTGGGGCCGGCCCGATCGACCGCCCGCCGTCGAGATCTGGACGCTGCCCGCGATCGGGCATGGCTTTCCCGTCGATGC
CAGCCTGCCCGGGAGCGGCCGCACCAGCCCGTATCTGGCGCGCGCGGATCTGTCCGCCGCGCGCCAGATCGCGACGTTCT
GGCTTCTGAACAATGACCACGCCAAACGCGCGGCCTTGCACCGCTGA

Upstream 100 bases:

>100_bases
CGCCACCAAAGTCGCGCGGCGGCAGGCCGCGGCGCTGGTAAAGGATGCCGCCCGCGTCTGGAAGCGGGCGTTGGTGGATC
GGCCGGTGTCGGCGGCTCGC

Downstream 100 bases:

>100_bases
ATGGGTTCGCCGATGCTCGTAACACCAGTACCACGGCTACGTTGATGATAGATCTGTCGCCGCCACGGGCGCGACCGGGC
ACGCCAGCCGCGATCAATGA

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSAGCVSEVVGFGSNPGALRMLVHARARLPAGRPLVVVLHGCGQDAAAFANASGWLALADELGFALLVPEQVHANNRGRC
FNWFRPDDVRRSGGEAMSIRQMLRVAVARYGSDPRRIYIVGFSAGGGMAAAMLAAYPAVFAAGGIVAGMPVGCAQTQVAA
MLHMRRANMSRSRQALAGDVRAVTQARSRRSWPRVSIWQGGRDRTVDPANAEILAAQWGELHGQDTAPRTDNVASGHRHR
TWGRPDRPPAVEIWTLPAIGHGFPVDASLPGSGRTSPYLARADLSAARQIATFWLLNNDHAKRAALHR

Sequences:

>Translated_308_residues
MSAGCVSEVVGFGSNPGALRMLVHARARLPAGRPLVVVLHGCGQDAAAFANASGWLALADELGFALLVPEQVHANNRGRC
FNWFRPDDVRRSGGEAMSIRQMLRVAVARYGSDPRRIYIVGFSAGGGMAAAMLAAYPAVFAAGGIVAGMPVGCAQTQVAA
MLHMRRANMSRSRQALAGDVRAVTQARSRRSWPRVSIWQGGRDRTVDPANAEILAAQWGELHGQDTAPRTDNVASGHRHR
TWGRPDRPPAVEIWTLPAIGHGFPVDASLPGSGRTSPYLARADLSAARQIATFWLLNNDHAKRAALHR
>Mature_307_residues
SAGCVSEVVGFGSNPGALRMLVHARARLPAGRPLVVVLHGCGQDAAAFANASGWLALADELGFALLVPEQVHANNRGRCF
NWFRPDDVRRSGGEAMSIRQMLRVAVARYGSDPRRIYIVGFSAGGGMAAAMLAAYPAVFAAGGIVAGMPVGCAQTQVAAM
LHMRRANMSRSRQALAGDVRAVTQARSRRSWPRVSIWQGGRDRTVDPANAEILAAQWGELHGQDTAPRTDNVASGHRHRT
WGRPDRPPAVEIWTLPAIGHGFPVDASLPGSGRTSPYLARADLSAARQIATFWLLNNDHAKRAALHR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 32989; Mature: 32858

Theoretical pI: Translated: 11.74; Mature: 11.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGCVSEVVGFGSNPGALRMLVHARARLPAGRPLVVVLHGCGQDAAAFANASGWLALAD
CCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCCCCEEEHH
ELGFALLVPEQVHANNRGRCFNWFRPDDVRRSGGEAMSIRQMLRVAVARYGSDPRRIYIV
HCCEEEEECHHHCCCCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
GFSAGGGMAAAMLAAYPAVFAAGGIVAGMPVGCAQTQVAAMLHMRRANMSRSRQALAGDV
EECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVTQARSRRSWPRVSIWQGGRDRTVDPANAEILAAQWGELHGQDTAPRTDNVASGHRHR
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCC
TWGRPDRPPAVEIWTLPAIGHGFPVDASLPGSGRTSPYLARADLSAARQIATFWLLNNDH
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEEEEECCCC
AKRAALHR
HHHHHCCC
>Mature Secondary Structure 
SAGCVSEVVGFGSNPGALRMLVHARARLPAGRPLVVVLHGCGQDAAAFANASGWLALAD
CCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHCCCCCCEEEHH
ELGFALLVPEQVHANNRGRCFNWFRPDDVRRSGGEAMSIRQMLRVAVARYGSDPRRIYIV
HCCEEEEECHHHCCCCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
GFSAGGGMAAAMLAAYPAVFAAGGIVAGMPVGCAQTQVAAMLHMRRANMSRSRQALAGDV
EECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAVTQARSRRSWPRVSIWQGGRDRTVDPANAEILAAQWGELHGQDTAPRTDNVASGHRHR
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCCC
TWGRPDRPPAVEIWTLPAIGHGFPVDASLPGSGRTSPYLARADLSAARQIATFWLLNNDH
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHEEEEECCCC
AKRAALHR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]