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The map label for this gene is merA [H]

Identifier: 148243661

GI number: 148243661

Start: 27999

End: 29513

Strand: Reverse

Name: merA [H]

Synonym: Acry_3132

Alternate gene names: 148243661

Gene position: 29513-27999 (Counterclockwise)

Preceding gene: 148243662

Following gene: 148243660

Centisome position: 14.5

GC content: 59.08

Gene sequence:

>1515_bases
ATGCAAGATGGCGTCCCAATAACGAAACCTGGCGACGAGGATCGCTTCCTTCGGAGGGTACGCCCTTCAGATTGGAAAAA
CCCGGTGCCACGGAACCCCTACGACATCGCAATTGTCGGAGCGGGCCCGGCCGGACTGGAAGCGGCAGAGTACGCCGCGC
GGCTGGGGTTCTCCGTCGCACTCATCGAGCGCAATCGCCTGGGCGGAAATTCGCTGAATGCAGGGTCGGTCCCATCCAAG
GCAATCATCTGCACCGCGCGCGTTTGTAGCACGATGCGGGATGCCGAAGCGTTCGGAGCGCCGATCCCGTCTGCGCCGTC
TCTCGATTTTGACAAGGTGATGGCGCGCATGCGCCGGATTCGGACGCGCATATCGGGATATCATTCGGTTCACGAACTCG
CCGAACCGGGTGTCGACATTTTTTTCGGTTCTGCCCGGTTCGAGAGCGCGGATACGCTGTTTGTGGACGACGCGCCCGTG
CATTTCAAAAAGGCGCTCATCGCCACAGGCGCGCGTCCCGGGGTGCCCAATATTCCGGGCCTCGATCAGACGGGTTATCG
CACGAGCGCGACGATCTTCGAGATGGCAGCACTGCCGACGCGTCTCGCCATCATCGGCGGAGGCCCGCTGGGTTGCGAAA
TGGCGCAAGCCTTTTGCGGGCTGGGATCGCACGTAACCATCGTGCAGAACGATCCGAAATTCCTGCCTCGCGAGGAACGT
GACGCCGCGGAAATCCTATCCCGCTCCATGGCGCGCGATGGAGTCGATATCCGTCTGAACACCACGGTGGTCGGGGCGCG
GCGCGTGAACGGCATCAAGATTCTGGAGACCGTCAATAACGATGTAAAGGGCGACATTCAAGCCGACGAAATCCTGTTGA
GCATCGGCCGGGTTCCAAATGTCGAAAAGCTCAATCTTGCAGCCGCCGGCGTCACTTTTGACGAGGACCATGGAATCAAA
GTTGATGATTTTCTGCGCAGCACAAATCCAAATGTGTACGCCGCAGGTGACGTCTGCTTGGCGCTTAAATTCACCAACGC
CGCACAGTCCTCAGCGCGCATGGCGGTTCGGAATGCGCTGATGCAGGCACAACAGCGACAAAGCAGCTTGGTCATACCCT
GGTGCACCTATTGCGATCCCGAAATCGCGCATATCGGTCTGCACGTCTGGGAAGCGCGCCAGCAATCGATTCCCATCAAG
AGTTTCACCGTGATGATGCACGACGTCGATCGCGCGATCACCGATGGACGGGACACGGGCTTCGTCAAGATCCACATATC
GGAAGGGTCCGACAGGATTTTAGGCGCGACCATCGTCTCCTCGCGGGCGAGCGAGTTGATTAACGAGATGGCCGTCATAA
TGAGCGCCGGCATCGGCATGAAGGCGCTGGCAGAGGTGGCACACACCTATCCGGCGCAATCCGAGGCAATCCTGCTTGCG
GCACAAGCCTATACGCGCAATTTCGATGAGGCGGCAAAAAGGGCTGCATCTGGAAACGCGGACAATGGAAAATGA

Upstream 100 bases:

>100_bases
AAAATCATCGCACTGATATGATCTGGGAGATCATGCGGCATTGCAGTTACGTGCACGATGGGCTCCAACGCGCCGGTTTT
GAAGACGGGTGGCTTTAGCA

Downstream 100 bases:

>100_bases
GGCCGCAGGTTGACCGGAAAAATTCTTCAGGCCTTTACCAATCTGGGTCTGATAAACGCTGGCTCGTTCTGATCCCGGCT
TGTGAATCAACGAGCTTCCG

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MQDGVPITKPGDEDRFLRRVRPSDWKNPVPRNPYDIAIVGAGPAGLEAAEYAARLGFSVALIERNRLGGNSLNAGSVPSK
AIICTARVCSTMRDAEAFGAPIPSAPSLDFDKVMARMRRIRTRISGYHSVHELAEPGVDIFFGSARFESADTLFVDDAPV
HFKKALIATGARPGVPNIPGLDQTGYRTSATIFEMAALPTRLAIIGGGPLGCEMAQAFCGLGSHVTIVQNDPKFLPREER
DAAEILSRSMARDGVDIRLNTTVVGARRVNGIKILETVNNDVKGDIQADEILLSIGRVPNVEKLNLAAAGVTFDEDHGIK
VDDFLRSTNPNVYAAGDVCLALKFTNAAQSSARMAVRNALMQAQQRQSSLVIPWCTYCDPEIAHIGLHVWEARQQSIPIK
SFTVMMHDVDRAITDGRDTGFVKIHISEGSDRILGATIVSSRASELINEMAVIMSAGIGMKALAEVAHTYPAQSEAILLA
AQAYTRNFDEAAKRAASGNADNGK

Sequences:

>Translated_504_residues
MQDGVPITKPGDEDRFLRRVRPSDWKNPVPRNPYDIAIVGAGPAGLEAAEYAARLGFSVALIERNRLGGNSLNAGSVPSK
AIICTARVCSTMRDAEAFGAPIPSAPSLDFDKVMARMRRIRTRISGYHSVHELAEPGVDIFFGSARFESADTLFVDDAPV
HFKKALIATGARPGVPNIPGLDQTGYRTSATIFEMAALPTRLAIIGGGPLGCEMAQAFCGLGSHVTIVQNDPKFLPREER
DAAEILSRSMARDGVDIRLNTTVVGARRVNGIKILETVNNDVKGDIQADEILLSIGRVPNVEKLNLAAAGVTFDEDHGIK
VDDFLRSTNPNVYAAGDVCLALKFTNAAQSSARMAVRNALMQAQQRQSSLVIPWCTYCDPEIAHIGLHVWEARQQSIPIK
SFTVMMHDVDRAITDGRDTGFVKIHISEGSDRILGATIVSSRASELINEMAVIMSAGIGMKALAEVAHTYPAQSEAILLA
AQAYTRNFDEAAKRAASGNADNGK
>Mature_504_residues
MQDGVPITKPGDEDRFLRRVRPSDWKNPVPRNPYDIAIVGAGPAGLEAAEYAARLGFSVALIERNRLGGNSLNAGSVPSK
AIICTARVCSTMRDAEAFGAPIPSAPSLDFDKVMARMRRIRTRISGYHSVHELAEPGVDIFFGSARFESADTLFVDDAPV
HFKKALIATGARPGVPNIPGLDQTGYRTSATIFEMAALPTRLAIIGGGPLGCEMAQAFCGLGSHVTIVQNDPKFLPREER
DAAEILSRSMARDGVDIRLNTTVVGARRVNGIKILETVNNDVKGDIQADEILLSIGRVPNVEKLNLAAAGVTFDEDHGIK
VDDFLRSTNPNVYAAGDVCLALKFTNAAQSSARMAVRNALMQAQQRQSSLVIPWCTYCDPEIAHIGLHVWEARQQSIPIK
SFTVMMHDVDRAITDGRDTGFVKIHISEGSDRILGATIVSSRASELINEMAVIMSAGIGMKALAEVAHTYPAQSEAILLA
AQAYTRNFDEAAKRAASGNADNGK

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=28.1995661605206, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=25.5913978494624, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=24.2553191489362, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI148277065, Length=503, Percent_Identity=22.0675944333996, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI148277071, Length=503, Percent_Identity=22.0675944333996, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI33519430, Length=475, Percent_Identity=22.5263157894737, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI33519428, Length=475, Percent_Identity=22.5263157894737, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI33519426, Length=475, Percent_Identity=22.5263157894737, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=28.8503253796095, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=29.3216630196937, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=27.1367521367521, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789915, Length=452, Percent_Identity=27.8761061946903, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789065, Length=216, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=27.2331154684096, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17557007, Length=477, Percent_Identity=26.2054507337526, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=23.0593607305936, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=23.0593607305936, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=476, Percent_Identity=23.3193277310924, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=28.2786885245902, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=25.2609603340292, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=23.2258064516129, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=28.1045751633987, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24640551, Length=464, Percent_Identity=24.3534482758621, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640549, Length=464, Percent_Identity=24.3534482758621, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640553, Length=464, Percent_Identity=24.3534482758621, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=24.8459958932238, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 54219; Mature: 54219

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDGVPITKPGDEDRFLRRVRPSDWKNPVPRNPYDIAIVGAGPAGLEAAEYAARLGFSVA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEE
LIERNRLGGNSLNAGSVPSKAIICTARVCSTMRDAEAFGAPIPSAPSLDFDKVMARMRRI
EEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RTRISGYHSVHELAEPGVDIFFGSARFESADTLFVDDAPVHFKKALIATGARPGVPNIPG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCC
LDQTGYRTSATIFEMAALPTRLAIIGGGPLGCEMAQAFCGLGSHVTIVQNDPKFLPREER
CCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
DAAEILSRSMARDGVDIRLNTTVVGARRVNGIKILETVNNDVKGDIQADEILLSIGRVPN
CHHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEHHCCCCCCCCCHHHHHHHHCCCCC
VEKLNLAAAGVTFDEDHGIKVDDFLRSTNPNVYAAGDVCLALKFTNAAQSSARMAVRNAL
CCEEEEEEECCEECCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHH
MQAQQRQSSLVIPWCTYCDPEIAHIGLHVWEARQQSIPIKSFTVMMHDVDRAITDGRDTG
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
FVKIHISEGSDRILGATIVSSRASELINEMAVIMSAGIGMKALAEVAHTYPAQSEAILLA
EEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
AQAYTRNFDEAAKRAASGNADNGK
EHHHHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQDGVPITKPGDEDRFLRRVRPSDWKNPVPRNPYDIAIVGAGPAGLEAAEYAARLGFSVA
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEE
LIERNRLGGNSLNAGSVPSKAIICTARVCSTMRDAEAFGAPIPSAPSLDFDKVMARMRRI
EEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RTRISGYHSVHELAEPGVDIFFGSARFESADTLFVDDAPVHFKKALIATGARPGVPNIPG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCC
LDQTGYRTSATIFEMAALPTRLAIIGGGPLGCEMAQAFCGLGSHVTIVQNDPKFLPREER
CCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
DAAEILSRSMARDGVDIRLNTTVVGARRVNGIKILETVNNDVKGDIQADEILLSIGRVPN
CHHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEHHCCCCCCCCCHHHHHHHHCCCCC
VEKLNLAAAGVTFDEDHGIKVDDFLRSTNPNVYAAGDVCLALKFTNAAQSSARMAVRNAL
CCEEEEEEECCEECCCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHH
MQAQQRQSSLVIPWCTYCDPEIAHIGLHVWEARQQSIPIKSFTVMMHDVDRAITDGRDTG
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
FVKIHISEGSDRILGATIVSSRASELINEMAVIMSAGIGMKALAEVAHTYPAQSEAILLA
EEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
AQAYTRNFDEAAKRAASGNADNGK
EHHHHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]