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The map label for this gene is tniR [H]

Identifier: 145301536

GI number: 145301536

Start: 149139

End: 149753

Strand: Reverse

Name: tniR [H]

Synonym: ASA_P5G160

Alternate gene names: 145301536

Gene position: 149753-149139 (Counterclockwise)

Preceding gene: 145301537

Following gene: 145301534

Centisome position: 96.55

GC content: 57.07

Gene sequence:

>615_bases
ATGCTGATTGGCTATATGCGGGTATCGAAAGCAGATGGTTCACAAGCAACTGACTTACAGCGTGATGCGCTGATCTCTGC
AGGCCTCGACCCCTCCCATCTCTACGAAGATCAGGCTTCCGGTAAACGAGAGGATCGCCCAGGACTGGCAGCCTGCCTGA
AGGCATTGCGCGAGGGCGATACATTGGTTGTATGGAAACTGGATCGTCTCGGGCGAGACCTGCGCCACCTGATCAACACA
GTCCACGACCTCACAGGGCGTGGCATTGGCCTCAAAGTGTTGACCGGTCACGGCGCGGCCATCGATACCACCACTGCCGC
AGGCAAGCTTGTATTTGGCATCTTCGCTGCGCTTGCTGAGTTTGAACGAGAGTTGATCGCCGAACGAACCATTGCGGGGT
TGGCATCAGCACGGGCACGCGGCAGGAAAGGTGGCCGACCGTTCAAGATGACCGCAGCCAAGCTACGGTTGGCGATGGCC
GCGATGGGGCAACCTGAAACCAAGGTCGGTGATCTGTGTCAGGAGCTAGGGATCACTCGGCAGACCTTATATCGACATGT
TTCGCCAAAAGGCGAGCTACGTGGTGATGGCGTAAAGCTGCTCAATGGTGGATGA

Upstream 100 bases:

>100_bases
TTCCTTAGAATAAATGACTGGTTAAGTGACCCACTTTGACGAACAAGCGCTTTCAATTCAAAATGTCACATAATCGAACG
TATATGTGACGGATGTAACT

Downstream 100 bases:

>100_bases
AGCGTTCTGGTAGTGCAGTCGGCTTTTGACATTCTGTGCAATCGTCTTCTGAAAATGACACGGTTACTAGCAAAGATGTG
TCAGGCTTTGCCAATGGCTT

Product: TniR protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLIGYMRVSKADGSQATDLQRDALISAGLDPSHLYEDQASGKREDRPGLAACLKALREGDTLVVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELIAERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHVSPKGELRGDGVKLLNGG

Sequences:

>Translated_204_residues
MLIGYMRVSKADGSQATDLQRDALISAGLDPSHLYEDQASGKREDRPGLAACLKALREGDTLVVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELIAERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHVSPKGELRGDGVKLLNGG
>Mature_204_residues
MLIGYMRVSKADGSQATDLQRDALISAGLDPSHLYEDQASGKREDRPGLAACLKALREGDTLVVWKLDRLGRDLRHLINT
VHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELIAERTIAGLASARARGRKGGRPFKMTAAKLRLAMA
AMGQPETKVGDLCQELGITRQTLYRHVSPKGELRGDGVKLLNGG

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=194, Percent_Identity=49.4845360824742, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=34.5744680851064, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787827, Length=188, Percent_Identity=35.1063829787234, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21870; Mature: 21870

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYMRVSKADGSQATDLQRDALISAGLDPSHLYEDQASGKREDRPGLAACLKALREGD
CEEECEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
TLVVWKLDRLGRDLRHLINTVHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAE
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
FERELIAERTIAGLASARARGRKGGRPFKMTAAKLRLAMAAMGQPETKVGDLCQELGITR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
QTLYRHVSPKGELRGDGVKLLNGG
HHHHHHCCCCCCCCCCCEEECCCC
>Mature Secondary Structure
MLIGYMRVSKADGSQATDLQRDALISAGLDPSHLYEDQASGKREDRPGLAACLKALREGD
CEEECEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
TLVVWKLDRLGRDLRHLINTVHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAE
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHH
FERELIAERTIAGLASARARGRKGGRPFKMTAAKLRLAMAAMGQPETKVGDLCQELGITR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHH
QTLYRHVSPKGELRGDGVKLLNGG
HHHHHHCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]