Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 145301534

GI number: 145301534

Start: 146301

End: 147923

Strand: Reverse

Name: eptA [H]

Synonym: ASA_P5G158

Alternate gene names: 145301534

Gene position: 147923-146301 (Counterclockwise)

Preceding gene: 145301536

Following gene: 145301533

Centisome position: 95.37

GC content: 41.34

Gene sequence:

>1623_bases
ATGCCTTTCATAATAAAAATTAAAATTGTGCCACTTGTTTTTTTGTTGGCATTTTATTTTGCATTCATGCTGAACTGGCG
CGGCGTTCTCCATTTTTATGATGTCCTCTACAAACTGGAAGACTTTAAGCTTGGGTTTGCAGTCTCCCTTCCAATTCTGT
TAGTTGCGGCACTAAACTTTGTTTTTGTCCCATTTTCATTTCGATATTTAGTGAAACCATTTTTTGCTCTTCTTCTCGTG
TTGAGTGCAATCGTCAGCTATACCATGATGAAGTATCGTGTCTTGTTTGATCAAAACATGATTCAGAACATTTTTGAAAC
GAACCAAAGCGAAGCATATGCCTACTTTAGCTTACCAATTTTGGGATGGGTTACGTTAGCAGGAATAATTCCAGCGGCAT
CATTGTTTCTTGTTAAAATTGAGTACGAGACAGAATGGTACAAAGGGATCATTCTACGAGTGTTATCCATGATTGCATCC
TTATTTATCATTGGCATTATCGCGACATTGTATTATCAGGATTATGTTTCAGTAGGACGAAATAATCCAAATTTACAGCG
TGAAATTGTCCCTGCAAACTTTATCAACAGTTCAACTAAATACATTTATAATCGCTATTTTGCAGAACCAATTCCTTTTT
CTACTTTGGGAGATGATGCTATACGGGCCATGGACAAAGATAAACCCACCCTAATGTTCCTAGTTGTGGGGGAGACTGCT
CGTGGTAAGAATTTCTCAATGAACGGTTATGAGAAGGATACAAACCCTTTTACCAGTAAAGTCGGTGGGGTCATCTCATT
TAAAGATGTGCGCTCGTGTGGAACTGCAACAGCAGTATCTGTGCCTTGCATGTTCTCCAATATGGGGAGAAAAGAGTTTG
ATGCTAATCAAGCTCGCAACAGTGAGGGTCTGCTAGATGTGTTGCAGAAAACGGGGATCTCCATTTTTTGGAAGGAAAAC
GATGGAGGCTGCAAAGGAGTCTGTGATCGAGTTCCTAACATCGAAATCAAGCCGAAGGATTATCCAAAGTTCTGTGATAA
GAACACATGCTATGACGAGGTTTTACTGCAAAACCTGGATGATGAGATCGCTCGGATGAAAGGGGACAAGCTGATTGGTT
TCCACTTGATAGGTAGCCATGGCCCAACCTACTACAAACGCTACCCTGATGCTTATCGCCAGTTCTTGCCTGATTGCCCA
CGTAGCGATATCGAAAACTGTACAGATGAAGAGCTTACAAACACCTATGACAATACCATTCGTTACACCGACTTCGTGAT
TGCAGAGATGATTGCCAAGTTGAAAACCTACGAAGATAAGTACAACACGGCCTTGATCTACGTCTCTGATCACGGTGAAT
CACTGGGAGCATTGGGGCTTTACCTGCACGGTACACCGTACAAGTTTGCACCAGATGACCAGACCCGTGTGCCTATGCAA
GTCTGGATGTCACCCAATTTTACCAAAGAGAAAGGGCTGGATATGAAGTGCTTGCAGAATAATTCAGCTATCTACCGCTA
TTCGCATGACAATCTTTTTCCATCTGTTTTGGGACTCTGGGACATTAAAACTAAGGTTTACGACAAGACCTTAGATATAT
TCAAACAATGTCGAAAAAACTGA

Upstream 100 bases:

>100_bases
ATTAATCTTCTGTACGGCTTTGTTACTATTCTACCGCAACATGTCGGGACAGGGTGTCTCGCCTATAGATTTATATTTTG
AGGCTGTAAATGGAGCTGTT

Downstream 100 bases:

>100_bases
ATTATGTGTCATGTGTTATTTTTAATAAAACAGTTCTAGTAGAATTGAAGGGTTTATCAGGGAGAAGTTATGTTTTCGGT
AGTCAAAATAAGAGTTGTTC

Product: putative sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MPFIIKIKIVPLVFLLAFYFAFMLNWRGVLHFYDVLYKLEDFKLGFAVSLPILLVAALNFVFVPFSFRYLVKPFFALLLV
LSAIVSYTMMKYRVLFDQNMIQNIFETNQSEAYAYFSLPILGWVTLAGIIPAASLFLVKIEYETEWYKGIILRVLSMIAS
LFIIGIIATLYYQDYVSVGRNNPNLQREIVPANFINSSTKYIYNRYFAEPIPFSTLGDDAIRAMDKDKPTLMFLVVGETA
RGKNFSMNGYEKDTNPFTSKVGGVISFKDVRSCGTATAVSVPCMFSNMGRKEFDANQARNSEGLLDVLQKTGISIFWKEN
DGGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVLLQNLDDEIARMKGDKLIGFHLIGSHGPTYYKRYPDAYRQFLPDCP
RSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
VWMSPNFTKEKGLDMKCLQNNSAIYRYSHDNLFPSVLGLWDIKTKVYDKTLDIFKQCRKN

Sequences:

>Translated_540_residues
MPFIIKIKIVPLVFLLAFYFAFMLNWRGVLHFYDVLYKLEDFKLGFAVSLPILLVAALNFVFVPFSFRYLVKPFFALLLV
LSAIVSYTMMKYRVLFDQNMIQNIFETNQSEAYAYFSLPILGWVTLAGIIPAASLFLVKIEYETEWYKGIILRVLSMIAS
LFIIGIIATLYYQDYVSVGRNNPNLQREIVPANFINSSTKYIYNRYFAEPIPFSTLGDDAIRAMDKDKPTLMFLVVGETA
RGKNFSMNGYEKDTNPFTSKVGGVISFKDVRSCGTATAVSVPCMFSNMGRKEFDANQARNSEGLLDVLQKTGISIFWKEN
DGGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVLLQNLDDEIARMKGDKLIGFHLIGSHGPTYYKRYPDAYRQFLPDCP
RSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
VWMSPNFTKEKGLDMKCLQNNSAIYRYSHDNLFPSVLGLWDIKTKVYDKTLDIFKQCRKN
>Mature_539_residues
PFIIKIKIVPLVFLLAFYFAFMLNWRGVLHFYDVLYKLEDFKLGFAVSLPILLVAALNFVFVPFSFRYLVKPFFALLLVL
SAIVSYTMMKYRVLFDQNMIQNIFETNQSEAYAYFSLPILGWVTLAGIIPAASLFLVKIEYETEWYKGIILRVLSMIASL
FIIGIIATLYYQDYVSVGRNNPNLQREIVPANFINSSTKYIYNRYFAEPIPFSTLGDDAIRAMDKDKPTLMFLVVGETAR
GKNFSMNGYEKDTNPFTSKVGGVISFKDVRSCGTATAVSVPCMFSNMGRKEFDANQARNSEGLLDVLQKTGISIFWKEND
GGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVLLQNLDDEIARMKGDKLIGFHLIGSHGPTYYKRYPDAYRQFLPDCPR
SDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQV
WMSPNFTKEKGLDMKCLQNNSAIYRYSHDNLFPSVLGLWDIKTKVYDKTLDIFKQCRKN

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=495, Percent_Identity=41.2121212121212, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI87082286, Length=550, Percent_Identity=24.7272727272727, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082223, Length=258, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61946; Mature: 61815

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFIIKIKIVPLVFLLAFYFAFMLNWRGVLHFYDVLYKLEDFKLGFAVSLPILLVAALNF
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VFVPFSFRYLVKPFFALLLVLSAIVSYTMMKYRVLFDQNMIQNIFETNQSEAYAYFSLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
LGWVTLAGIIPAASLFLVKIEYETEWYKGIILRVLSMIASLFIIGIIATLYYQDYVSVGR
HHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNPNLQREIVPANFINSSTKYIYNRYFAEPIPFSTLGDDAIRAMDKDKPTLMFLVVGETA
CCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
RGKNFSMNGYEKDTNPFTSKVGGVISFKDVRSCGTATAVSVPCMFSNMGRKEFDANQARN
CCCCCCCCCCCCCCCCHHHHHCCCEEHHHHHHCCCCHHEECCHHHHCCCCCCCCHHHCCC
SEGLLDVLQKTGISIFWKENDGGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVLLQNLD
CCHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHCCC
DEIARMKGDKLIGFHLIGSHGPTYYKRYPDAYRQFLPDCPRSDIENCTDEELTNTYDNTI
HHHHHHCCCCEEEEEEECCCCCHHHHCCCHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCH
RYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEE
VWMSPNFTKEKGLDMKCLQNNSAIYRYSHDNLFPSVLGLWDIKTKVYDKTLDIFKQCRKN
EECCCCCCHHCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFIIKIKIVPLVFLLAFYFAFMLNWRGVLHFYDVLYKLEDFKLGFAVSLPILLVAALNF
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VFVPFSFRYLVKPFFALLLVLSAIVSYTMMKYRVLFDQNMIQNIFETNQSEAYAYFSLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHH
LGWVTLAGIIPAASLFLVKIEYETEWYKGIILRVLSMIASLFIIGIIATLYYQDYVSVGR
HHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNPNLQREIVPANFINSSTKYIYNRYFAEPIPFSTLGDDAIRAMDKDKPTLMFLVVGETA
CCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCC
RGKNFSMNGYEKDTNPFTSKVGGVISFKDVRSCGTATAVSVPCMFSNMGRKEFDANQARN
CCCCCCCCCCCCCCCCHHHHHCCCEEHHHHHHCCCCHHEECCHHHHCCCCCCCCHHHCCC
SEGLLDVLQKTGISIFWKENDGGCKGVCDRVPNIEIKPKDYPKFCDKNTCYDEVLLQNLD
CCHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHHCCC
DEIARMKGDKLIGFHLIGSHGPTYYKRYPDAYRQFLPDCPRSDIENCTDEELTNTYDNTI
HHHHHHCCCCEEEEEEECCCCCHHHHCCCHHHHHHCCCCCCHHHCCCCHHHHHHHHCCCH
RYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEE
VWMSPNFTKEKGLDMKCLQNNSAIYRYSHDNLFPSVLGLWDIKTKVYDKTLDIFKQCRKN
EECCCCCCHHCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]