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The map label for this gene is eptA [H]

Identifier: 145301533

GI number: 145301533

Start: 144606

End: 146231

Strand: Reverse

Name: eptA [H]

Synonym: ASA_P5G157

Alternate gene names: 145301533

Gene position: 146231-144606 (Counterclockwise)

Preceding gene: 145301534

Following gene: 145301532

Centisome position: 94.28

GC content: 42.87

Gene sequence:

>1626_bases
ATGTTTTCGGTAGTCAAAATAAGAGTTGTTCCATTCATTTTATTGTTGGCCTTTGTTTTTTCGTTTTTACTAAACTGGCC
AGTTTTGCTCCATTTTTATGACATTCTGTCCAACCTTGAACACTTTAAGGTCGGTTTTGTTATTTCAATTCCTTTTTTAC
TTGTTGCGGCTCTTAACTTCGTCTTCATGCCATTTTCTATCCGCTATCTGATGAAACCCTTCTTCGCCTTCCTCTTTGTG
ATGGGTTCTATCGCTAGCTATACCATGATGAAATATCGGGTATTGTTTGATGGTGACATGATTCAGAATATTTTTGAAAC
AAATCAAAGTGAAGCATATGCCTACGTAAACCTATCTATCATTATGTGGGTTCTGTTTACAGGAGTGCTTCCTGCCATAT
TAATTTTCTTTGTGAAAATTGAGTATGCATCTACTTGGTATAAAGGTATTGCTCAGAGAGCACTCTCTATGCTGGCCTCG
TTAGCAATAGTTGGGGTTATTGCGGCATTGTACTATCAGGATTATGCATCTATTGGCCGCAACAATCAGACTCTAAATCG
TGAAATTGTCCCAGCCAACTTTATGTATAGCACCAGTAAATATCTTTATAGACGCTATATGGCTGAGCCTGTCCCCTTTA
CTACTTTGGGCGATGACGCTACACGAGTAGCGAAAGAAGACAAGCCCACTCTGATGTTTTTGGTTGTTGGTGAAACCGCT
CGTGGTAAAAATTTCTCCATGAATGGCTACGAGAAAGAAACTAACGCTTTTACCAGTAAGATTGAGGATGTCATTTCTTT
TAACGATGTTCGTTCATGTGGCACTGCTACAGCAGTATCGGTGCCCTGCATGTTCTCCAATATGGGGAGAAAGGAGTTTG
ATGATAATCGAGCTCGCAATAGTGAAGGGCTACTTGATGTATTGAATAAAACCGGGATCTCCATCTTTTGGAAAGAGAAC
GATGGCGGATGCAAAGGTGTTTGCGATAGAGTCCCGAATGTCGAGGTCAAGCCGAAGGATCATCCTAAGTTCTGCGATAA
AAACACATGCTATGACGAGGTTGTCCTCCAAGGTCTTGATAATGAGATCGCTCAAATGAAAGGAGATAAACTGGTTGGCT
TCCACCTCATAGGCAGCCATGGCCCAACCTACTACAAACGCTACCCTGATGCTCATCGCCAGTTCCTGCCTGATTGCCCA
CGTAGCGATATTGAAAACTGTACAGATGAAGAGCTTACAAACACCTATGACAACACCATTCGCTACACCGACTTCGTGAT
TGCAGAGATGATTGCCAAGTTGAAGACTTACGAAGATAAGTACAACACCGCGTTGATCTATGTCTCCGATCACGGTGAAT
CATTGGGGGCATTGGGGCTTTACCTGCACGGTACACCGTACAAGTTTGCACCGGATGACCAGACCCGAGTACCTATGCAG
GTCTGGATGTCACCAGGATTTACCAAAGAGAAAGGGATTGATATGACGTGTTTGCAGCAGAAAGCTGCTGATACCCGTTA
CTCACACGATAATATTTTTTCATCTGTGCTGGGTATCTGGGACATCAAAACATCAGCTTACGAGCAGGGGCTCGATATTT
TCAGTCAATGCCGTCTTGTGCAATGA

Upstream 100 bases:

>100_bases
AGATATATTCAAACAATGTCGAAAAAACTGAATTATGTGTCATGTGTTATTTTTAATAAAACAGTTCTAGTAGAATTGAA
GGGTTTATCAGGGAGAAGTT

Downstream 100 bases:

>100_bases
GTATTATTTTGGGGGCTCAAGCCACCAAAAATCTGTTCAATAGTTAGTTTCATGACCATTGCGCATATGTTTCATTGTTG
AAACTCGGCGTGGGGCAATG

Product: putative sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MFSVVKIRVVPFILLLAFVFSFLLNWPVLLHFYDILSNLEHFKVGFVISIPFLLVAALNFVFMPFSIRYLMKPFFAFLFV
MGSIASYTMMKYRVLFDGDMIQNIFETNQSEAYAYVNLSIIMWVLFTGVLPAILIFFVKIEYASTWYKGIAQRALSMLAS
LAIVGVIAALYYQDYASIGRNNQTLNREIVPANFMYSTSKYLYRRYMAEPVPFTTLGDDATRVAKEDKPTLMFLVVGETA
RGKNFSMNGYEKETNAFTSKIEDVISFNDVRSCGTATAVSVPCMFSNMGRKEFDDNRARNSEGLLDVLNKTGISIFWKEN
DGGCKGVCDRVPNVEVKPKDHPKFCDKNTCYDEVVLQGLDNEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFLPDCP
RSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
VWMSPGFTKEKGIDMTCLQQKAADTRYSHDNIFSSVLGIWDIKTSAYEQGLDIFSQCRLVQ

Sequences:

>Translated_541_residues
MFSVVKIRVVPFILLLAFVFSFLLNWPVLLHFYDILSNLEHFKVGFVISIPFLLVAALNFVFMPFSIRYLMKPFFAFLFV
MGSIASYTMMKYRVLFDGDMIQNIFETNQSEAYAYVNLSIIMWVLFTGVLPAILIFFVKIEYASTWYKGIAQRALSMLAS
LAIVGVIAALYYQDYASIGRNNQTLNREIVPANFMYSTSKYLYRRYMAEPVPFTTLGDDATRVAKEDKPTLMFLVVGETA
RGKNFSMNGYEKETNAFTSKIEDVISFNDVRSCGTATAVSVPCMFSNMGRKEFDDNRARNSEGLLDVLNKTGISIFWKEN
DGGCKGVCDRVPNVEVKPKDHPKFCDKNTCYDEVVLQGLDNEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFLPDCP
RSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
VWMSPGFTKEKGIDMTCLQQKAADTRYSHDNIFSSVLGIWDIKTSAYEQGLDIFSQCRLVQ
>Mature_541_residues
MFSVVKIRVVPFILLLAFVFSFLLNWPVLLHFYDILSNLEHFKVGFVISIPFLLVAALNFVFMPFSIRYLMKPFFAFLFV
MGSIASYTMMKYRVLFDGDMIQNIFETNQSEAYAYVNLSIIMWVLFTGVLPAILIFFVKIEYASTWYKGIAQRALSMLAS
LAIVGVIAALYYQDYASIGRNNQTLNREIVPANFMYSTSKYLYRRYMAEPVPFTTLGDDATRVAKEDKPTLMFLVVGETA
RGKNFSMNGYEKETNAFTSKIEDVISFNDVRSCGTATAVSVPCMFSNMGRKEFDDNRARNSEGLLDVLNKTGISIFWKEN
DGGCKGVCDRVPNVEVKPKDHPKFCDKNTCYDEVVLQGLDNEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFLPDCP
RSDIENCTDEELTNTYDNTIRYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
VWMSPGFTKEKGIDMTCLQQKAADTRYSHDNIFSSVLGIWDIKTSAYEQGLDIFSQCRLVQ

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=496, Percent_Identity=41.3306451612903, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI87082286, Length=471, Percent_Identity=26.963906581741, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082223, Length=290, Percent_Identity=26.2068965517241, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790392, Length=404, Percent_Identity=22.7722772277228, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61648; Mature: 61648

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVVKIRVVPFILLLAFVFSFLLNWPVLLHFYDILSNLEHFKVGFVISIPFLLVAALNF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VFMPFSIRYLMKPFFAFLFVMGSIASYTMMKYRVLFDGDMIQNIFETNQSEAYAYVNLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCEEEEEHHHH
IMWVLFTGVLPAILIFFVKIEYASTWYKGIAQRALSMLASLAIVGVIAALYYQDYASIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNQTLNREIVPANFMYSTSKYLYRRYMAEPVPFTTLGDDATRVAKEDKPTLMFLVVGETA
CCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHCCCCCEEEEEEECCCC
RGKNFSMNGYEKETNAFTSKIEDVISFNDVRSCGTATAVSVPCMFSNMGRKEFDDNRARN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCHHHHCCCCCCCCCCCCCC
SEGLLDVLNKTGISIFWKENDGGCKGVCDRVPNVEVKPKDHPKFCDKNTCYDEVVLQGLD
CCHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
NEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFLPDCPRSDIENCTDEELTNTYDNTI
HHHHHHCCCCEEEEEEECCCCCHHHHCCCCHHHHHCCCCCCHHHCCCCHHHHHHHHCCCH
RYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHH
VWMSPGFTKEKGIDMTCLQQKAADTRYSHDNIFSSVLGIWDIKTSAYEQGLDIFSQCRLV
EECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
Q
C
>Mature Secondary Structure
MFSVVKIRVVPFILLLAFVFSFLLNWPVLLHFYDILSNLEHFKVGFVISIPFLLVAALNF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VFMPFSIRYLMKPFFAFLFVMGSIASYTMMKYRVLFDGDMIQNIFETNQSEAYAYVNLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCEEEEEHHHH
IMWVLFTGVLPAILIFFVKIEYASTWYKGIAQRALSMLASLAIVGVIAALYYQDYASIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNQTLNREIVPANFMYSTSKYLYRRYMAEPVPFTTLGDDATRVAKEDKPTLMFLVVGETA
CCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHCCCCCEEEEEEECCCC
RGKNFSMNGYEKETNAFTSKIEDVISFNDVRSCGTATAVSVPCMFSNMGRKEFDDNRARN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCHHHHCCCCCCCCCCCCCC
SEGLLDVLNKTGISIFWKENDGGCKGVCDRVPNVEVKPKDHPKFCDKNTCYDEVVLQGLD
CCHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
NEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFLPDCPRSDIENCTDEELTNTYDNTI
HHHHHHCCCCEEEEEEECCCCCHHHHCCCCHHHHHCCCCCCHHHCCCCHHHHHHHHCCCH
RYTDFVIAEMIAKLKTYEDKYNTALIYVSDHGESLGALGLYLHGTPYKFAPDDQTRVPMQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHH
VWMSPGFTKEKGIDMTCLQQKAADTRYSHDNIFSSVLGIWDIKTSAYEQGLDIFSQCRLV
EECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]