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The map label for this gene is insF1 [H]

Identifier: 145301530

GI number: 145301530

Start: 143186

End: 143914

Strand: Reverse

Name: insF1 [H]

Synonym: ASA_P5G154

Alternate gene names: 145301530

Gene position: 143914-143186 (Counterclockwise)

Preceding gene: 145301531

Following gene: 145301529

Centisome position: 92.79

GC content: 54.32

Gene sequence:

>729_bases
TTGGTCAAGCAATCCTGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGCGATCTATGTGACTTGGGGGA
AGCCTGCGGCAAGCACCGTGTGGCCAAATTAATGAGGCGGGAGGGGCTGCGTTCGCAAACTGGCTATCAACGCCGTAAGG
GGCATTATGGAGGCAAGAGCCCACAGGCTGCCCCAAACACGCTGGCACGTCAATTTAAGGTGCCAGCCCCCAACATCTCC
TGGGTGACTGACATTACCTACATCCGAACTCAAGAAGGGTGGTTGTACCTGGCGGTGGTGCTCGATCTGTTTTCTCGCCA
GATCATCGGCTGGGCGATGAAGCCACGCATGACGGCAGGCTTGGCGGTTGATGCGTTATTGATGGCGGTTTGGCGACGTA
AGCCAACGCAGCCGGTACTGGTGCATTCGGATCAGGGCAGCCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCC
CACAACCTGAGTTGCAGTATGAGCCGACGTGGTAACTGCCACGACAATGCTGTCGCCGAGAGCTTCTTTCAGTTGCTCAA
ACGGGAGCGGATCAAGCGCAGGATTTACTCGACAAGAGACGAAGCCCGCAGCGACGTATTTGATTATATCGAAATGTTTT
ACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAGGCAGTTTATTTGTCAGCAACAG
AGTGTCTAG

Upstream 100 bases:

>100_bases
TACCCTGTGCCAGACCCTGGGGGTTCACCGAAGTGGCTTCTACGCGTGGCTGAAACAGCCCGCATCGTTACGCCAGCAAG
CGGATGAACGGCTGACCGGA

Downstream 100 bases:

>100_bases
AAAACCAGGGGCGATTCAGTCAAAAGAAAGTTTTAGGTGAGCGAAAAAATGTCGTTTAACAATGATGCTGATAATGCGTA
CTTGGAGATCCTGCTGAAAA

Product: IS3 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSPQAAPNTLARQFKVPAPNIS
WVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLKA
HNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQ
SV

Sequences:

>Translated_242_residues
MVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSPQAAPNTLARQFKVPAPNIS
WVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLKA
HNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQ
SV
>Mature_242_residues
MVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKSPQAAPNTLARQFKVPAPNIS
WVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLKA
HNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQ
SV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=191, Percent_Identity=35.0785340314136, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1788405, Length=234, Percent_Identity=44.8717948717949, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1787263, Length=234, Percent_Identity=44.8717948717949, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1786753, Length=234, Percent_Identity=44.8717948717949, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1786570, Length=234, Percent_Identity=44.8717948717949, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1786490, Length=234, Percent_Identity=44.8717948717949, Blast_Score=194, Evalue=3e-51,
Organism=Escherichia coli, GI1789981, Length=233, Percent_Identity=35.1931330472103, Blast_Score=141, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 27944; Mature: 27944

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKS
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCC
PQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAG
CCCCHHHHHHHHCCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSRRGNCHDNAVAE
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
SFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
SV
CC
>Mature Secondary Structure
MVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGKS
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCC
PQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAG
CCCCHHHHHHHHCCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSRRGNCHDNAVAE
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
SFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
SV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]