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The map label for this gene is klcA [H]

Identifier: 145301509

GI number: 145301509

Start: 127874

End: 128278

Strand: Reverse

Name: klcA [H]

Synonym: ASA_P5G131

Alternate gene names: 145301509

Gene position: 128278-127874 (Counterclockwise)

Preceding gene: 145301510

Following gene: 145301507

Centisome position: 82.71

GC content: 40.74

Gene sequence:

>405_bases
ATGGATGCTTTAATTGCTGGTCTGGTTATTGATTCTAAACGTATGGACATGATGCCTAAATACTTCGGACGCTGGATGAT
GAAAACTGAAGGTAACATTTATGGCTGGATGAAAGAAATCTGCGCATCGTACAGCGGCGGTTATTGGGATATGTGGGAGG
TATCGAATGGTGCATTCTTTATGGTTCCCCCTTTGAAAAAAGAAGGTTACACACTATCAATTAATGGTAATGGTTTTGAG
GGAGTGTTGACTGCCCACGCTACCGGTGTTGTGGCTTGTCTTTTTGCATACTATTACATGTGGGAAATGACAGACGATGA
TCTCTTTGTAAGACTCTATCACGCACTTCGTGAATATGCATTGACGTTAGAAGAGCATAGTTTGATTTTTAGCGCATTAG
ATTAA

Upstream 100 bases:

>100_bases
TAAGGCGATTGCGGCGGCGCAGTGGCATTAAAATTCAGGCAACAAAAACCATCAATGTATAACGGTCTGGGAGTGGCCCA
GACCTAACAAGGGGTGATGT

Downstream 100 bases:

>100_bases
TCAGGTTTGCTTCCCTTGCTGTTATTTGGCCCCTGATGTTTGGGGCCTTTTTTGATCTCGTCATACGTGAGCTTGACCCG
TTCGGCGTACCATTTACGTT

Product: antirestriction protein KlcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MDALIAGLVIDSKRMDMMPKYFGRWMMKTEGNIYGWMKEICASYSGGYWDMWEVSNGAFFMVPPLKKEGYTLSINGNGFE
GVLTAHATGVVACLFAYYYMWEMTDDDLFVRLYHALREYALTLEEHSLIFSALD

Sequences:

>Translated_134_residues
MDALIAGLVIDSKRMDMMPKYFGRWMMKTEGNIYGWMKEICASYSGGYWDMWEVSNGAFFMVPPLKKEGYTLSINGNGFE
GVLTAHATGVVACLFAYYYMWEMTDDDLFVRLYHALREYALTLEEHSLIFSALD
>Mature_134_residues
MDALIAGLVIDSKRMDMMPKYFGRWMMKTEGNIYGWMKEICASYSGGYWDMWEVSNGAFFMVPPLKKEGYTLSINGNGFE
GVLTAHATGVVACLFAYYYMWEMTDDDLFVRLYHALREYALTLEEHSLIFSALD

Specific function: Could be involved in overcoming restriction barriers during establishment after conjugative transfer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the antirestriction protein family [H]

Homologues:

Organism=Escherichia coli, GI1786442, Length=135, Percent_Identity=36.2962962962963, Blast_Score=87, Evalue=5e-19,
Organism=Escherichia coli, GI1788996, Length=130, Percent_Identity=33.0769230769231, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004914
- ProDom:   PD012854 [H]

Pfam domain/function: PF03230 Antirestrict [H]

EC number: NA

Molecular weight: Translated: 15423; Mature: 15423

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
8.2 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
8.2 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALIAGLVIDSKRMDMMPKYFGRWMMKTEGNIYGWMKEICASYSGGYWDMWEVSNGAFF
CCCHHEEEEECCHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCEE
MVPPLKKEGYTLSINGNGFEGVLTAHATGVVACLFAYYYMWEMTDDDLFVRLYHALREYA
EECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LTLEEHSLIFSALD
HHHHHHHEEEEECC
>Mature Secondary Structure
MDALIAGLVIDSKRMDMMPKYFGRWMMKTEGNIYGWMKEICASYSGGYWDMWEVSNGAFF
CCCHHEEEEECCHHHHCCHHHHCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCEE
MVPPLKKEGYTLSINGNGFEGVLTAHATGVVACLFAYYYMWEMTDDDLFVRLYHALREYA
EECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LTLEEHSLIFSALD
HHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7773415 [H]