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The map label for this gene is tnpA

Identifier: 145301427

GI number: 145301427

Start: 48181

End: 49395

Strand: Reverse

Name: tnpA

Synonym: ASA_P5G044

Alternate gene names: NA

Gene position: 49395-48181 (Counterclockwise)

Preceding gene: 145301428

Following gene: 145301404

Centisome position: 31.85

GC content: 56.95

Gene sequence:

>1215_bases
ATGGACCAAGACAAACTCAAAGCACTGGCCGCTGAACTGGCCAAAGACATCAAGTCTGAAAAAGATCTCGGCACCCTCAC
CCAACAGCTGATCAAGCTCACCGTCGAGACCGCGCTCAATGCCGAGATGGACGAGCATCTGGGCTACGAGAAACACGCTC
CACAAGGCCGCGGGACCGGCAACAATCGCAATGGCTATTCCACCAAGCGCCTAAAAGGCCAGCACGGAGAGGTCACCATC
CAGGCGCCTCGCGACCGTAACGCCTCCTTCGAGCCTCAGTTCGTCCGCAAGGGCCAGTCCCGCCTGACCCAGATGGACGA
CCAGATCCTCGCCCTCTACGCCAAGGGCTTGAGTACGCGGGATATCGTGGACGCGTTCAAAGAGATGTATGACGCCGACA
TCTCGGCGGGGCTGGTATCGAAGGTGACCGAACGTGTCATCGAGCAGGTCCACGAATGGCAAAACCGGCCGCTGGATCCG
CTCTATCCCATCGTCTATCTGGACTGCATTGTGCTGAAGATCCGCGCGAACCAGCGGGTGATCAACAAGTCGCTCTATTT
GGCTCTGGGCATCAATATGGAGGGGCACAAGGAGCTGTTGGGCCTGTGGCTGGCCGAGACCGAAGGGGCAAAGTTCTGGT
TGTCGGTCCTGACCGAGCTGAAGAACCGCGGCCTGGAAGACATCCTGCTCGCGTGTGTGGATGGTCTCAAGGGTTTCCCT
GATGCCATTGCCGTGGAGTATCCCCAGACCAAGGTGCAACTGTGTATCGTCCATATGGTCCGCAACTCGTTGCGCTATGT
CTCCTGGAAGGACTACAAGGCGGTGACGGCTGAACTGAAGCAGATCTACCAGTCTGCGACGGAACGTGAGGCTCAGCAGG
CGTTAGCGACCTTCGGCGAGCGCTGGGATAGCCAGTACCCGCAGATAGCGCGCTCGTGGCAGAATAACTGGGACAATCTG
ATCACGCTGTTTGATTATCCCCCCGCCATCTGGAAGGTAATTTACACAACCAATGCGATCGAGTCACTCAACAGCGTGCT
GCGCAAAGCGACCAAACAAAGGAAGCTGTTCCCGACCGATGAGTCGGCGCTCAAAGTGGCGTTTCTCGCGATACAGCAGG
CTTCAAAGAAGTGGACCATGCCAATCCAGAATTGGAAGCCTGCCCTGAATCGCTTTATTATCGAGTTCGGTGATCGCCTG
AACGGTCACCTGTAA

Upstream 100 bases:

>100_bases
AATTTTTTGTTTAGTCAGCATTAATGAGCCTGTAAATCCAATTGTGTAACCGCCCACGAATAAGTAATTTTTTCAAAAGG
ATACCTTCGTGGGTAAGCAA

Downstream 100 bases:

>100_bases
TGGAGTGGGCAGTTACACAGAATTATTTACAGGCTCTCCACTGATGATGAAAAAAGTTAACTGCACGTAGCAGTCGCTGA
TGTAAAAAACCGGCCCATGG

Product: IS256 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTGNNRNGYSTKRLKGQHGEVTI
QAPRDRNASFEPQFVRKGQSRLTQMDDQILALYAKGLSTRDIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDP
LYPIVYLDCIVLKIRANQRVINKSLYLALGINMEGHKELLGLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFP
DAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGERWDSQYPQIARSWQNNWDNL
ITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTDESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRL
NGHL

Sequences:

>Translated_404_residues
MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTGNNRNGYSTKRLKGQHGEVTI
QAPRDRNASFEPQFVRKGQSRLTQMDDQILALYAKGLSTRDIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDP
LYPIVYLDCIVLKIRANQRVINKSLYLALGINMEGHKELLGLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFP
DAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGERWDSQYPQIARSWQNNWDNL
ITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTDESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRL
NGHL
>Mature_404_residues
MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTGNNRNGYSTKRLKGQHGEVTI
QAPRDRNASFEPQFVRKGQSRLTQMDDQILALYAKGLSTRDIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDP
LYPIVYLDCIVLKIRANQRVINKSLYLALGINMEGHKELLGLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFP
DAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGERWDSQYPQIARSWQNNWDNL
ITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTDESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRL
NGHL

Specific function: Required for the transposition of the insertion element [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase mutator family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001207 [H]

Pfam domain/function: PF00872 Transposase_mut [H]

EC number: NA

Molecular weight: Translated: 46121; Mature: 46121

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTG
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC
NNRNGYSTKRLKGQHGEVTIQAPRDRNASFEPQFVRKGQSRLTQMDDQILALYAKGLSTR
CCCCCCCHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDPLYPIVYLDCIVLKIRANQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCHHH
INKSLYLALGINMEGHKELLGLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFP
HHHHEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
DAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGE
CCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWDSQYPQIARSWQNNWDNLITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTD
HHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRLNGHL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQDKLKALAAELAKDIKSEKDLGTLTQQLIKLTVETALNAEMDEHLGYEKHAPQGRGTG
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCC
NNRNGYSTKRLKGQHGEVTIQAPRDRNASFEPQFVRKGQSRLTQMDDQILALYAKGLSTR
CCCCCCCHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DIVDAFKEMYDADISAGLVSKVTERVIEQVHEWQNRPLDPLYPIVYLDCIVLKIRANQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCHHH
INKSLYLALGINMEGHKELLGLWLAETEGAKFWLSVLTELKNRGLEDILLACVDGLKGFP
HHHHEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
DAIAVEYPQTKVQLCIVHMVRNSLRYVSWKDYKAVTAELKQIYQSATEREAQQALATFGE
CCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWDSQYPQIARSWQNNWDNLITLFDYPPAIWKVIYTTNAIESLNSVLRKATKQRKLFPTD
HHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ESALKVAFLAIQQASKKWTMPIQNWKPALNRFIIEFGDRLNGHL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1849509; 1327271; 11481430; 11481432 [H]