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The map label for this gene is garD [H]

Identifier: 159185399

GI number: 159185399

Start: 2824712

End: 2826244

Strand: Direct

Name: garD [H]

Synonym: Atu2822

Alternate gene names: 159185399

Gene position: 2824712-2826244 (Clockwise)

Preceding gene: 15890067

Following gene: 159185403

Centisome position: 99.41

GC content: 59.17

Gene sequence:

>1533_bases
GTGAGTGAGAATTCCGAGGCAACCATTTTGCTGAACGCCAACGACAATGTCGCGGTGGCGCGTCGGTCTATCCCGGCTGG
CGGAGCGACGGGCAGGGGTGATCTGGCGGCACTCGAGCTGATCGGACGCGGGCACAAGGTGGCGCTCAAACTCATCAAAT
CCGGAGACGAGGTGCTGAAATACGGGCAGGTCATCGGCATCGCGACCCAGGACATCGAAGCCGGCCGGCATGTGCACCTG
CACAATCTCGCCATGGTGCCGTCAGAACATGCGCATCAGTTCAGCGTCGACATCGAGGAGAAGGGCATGGTGCCGGAAGC
CGAGCGCCGGACCTTCATGGGTTATGACCGTGGTCTCGGCGGCGTTGGCACACGCAACTATATCGGCGTCATCGCCTCGG
TGAACTGTTCCGCCACTGTGTCGCGCTACATCGCCGATTATTTCAACAAACAGGGCGGCCTTGACGGCTTCGACAATGTC
GATGGCGTCGTGGCGCTGACCCACGGTGGCGGCTGTGCGCTGAACACCAAGTCGGAAGGTTACCGCCTTCTGATCCGCAC
CATTCAGGGTTATGCCCGCCATCCGAATTTCGGCGGGATTCTGCTGATCGGCCTTGGTTGCGAAACCAACCAGATCGCGC
CCATTCTCGAACATTACAAGATGGAAGAGGGCGAGCGGCTGCGCACCATGACCATCCAGCAGCATGGTGGCACCCGCAAG
ACCATCGATGCGGCAATAACCGAAATCAAGGCGATGCTACCGATGGTGAACGCCGCCCGCCGCACCGAGCAACCGCTTTC
CAAGCTGAAGGTCGCACTGGAATGTGGCGGCTCGGATGGATATTCCGGCATCTCCGCCAATCCGGCTCTGGGTTATGCAT
CCGACCTAATCGTCCGCAATGGCGGCACGACCGTGCTTTCCGAAACGCCCGAAATCTACGGCGCGGAGCACCTGCTGACC
AAACGCGCCGTCACACCTGCGGTCGCCGAAAAACTGCTGCAGCGCATCGACTGGTGGCGGGACTATACCGCCCGCAATGG
CGACGAACTCAACAACAATCCTTCGCACGGCAACAAGCTCGGCGGCCTGACTACCATTCTGGAGAAATCGCTGGGCGCGG
TCGCCAAGGGTGGCTCCATGCCGCTGAAGGCGGTTTATGAATTTGCCGAGACCGTGACGGAGCAAGGCTTCGTCTTCATG
GATACACCCGGCTATGACCCCGTTGCCGTCACCGGCCAGGTTGCCGGCGGCTGCAACGTCATCTGTTTCACCACCGGCCG
TGGCTCGGTTTCAGGTTTCAAGCCCTCTCCCTGCATCAAGATCGCCACCAACTCCGAAATGTATGAACACATGAAGGAAG
ACATGGATATCAACTGCGGCGAGATCGTCACCGGAACGGAAACCATCGAAGAATCCGGCAAACGAATTTTCGAACATATT
CTCGCGGTCGCTTCCGGCGACAAGACGGTGAGCGAAATTTATGATTACGGTGACAATGAATTTGTGCCGTGGCAGGTCGG
CGCAGTGACCTGA

Upstream 100 bases:

>100_bases
GCAAGCCGCCATGCACATCCATCTGGAAGGTTCGAAACGCCGCTGGGTGGATAGCTGAATGACAAACATAGCAGGCAATG
TACCGGGAGGAGGAAAACCC

Downstream 100 bases:

>100_bases
AGCGCAATACCGACTGTCCCACGTGAAACAAAAGGCCCGGAAATCACTTTCCGGGCCTTCGTCATTTCTGTCTATATCGC
GCGCTTAATGGCGGAAGTGG

Product: D-galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSENSEATILLNANDNVAVARRSIPAGGATGRGDLAALELIGRGHKVALKLIKSGDEVLKYGQVIGIATQDIEAGRHVHL
HNLAMVPSEHAHQFSVDIEEKGMVPEAERRTFMGYDRGLGGVGTRNYIGVIASVNCSATVSRYIADYFNKQGGLDGFDNV
DGVVALTHGGGCALNTKSEGYRLLIRTIQGYARHPNFGGILLIGLGCETNQIAPILEHYKMEEGERLRTMTIQQHGGTRK
TIDAAITEIKAMLPMVNAARRTEQPLSKLKVALECGGSDGYSGISANPALGYASDLIVRNGGTTVLSETPEIYGAEHLLT
KRAVTPAVAEKLLQRIDWWRDYTARNGDELNNNPSHGNKLGGLTTILEKSLGAVAKGGSMPLKAVYEFAETVTEQGFVFM
DTPGYDPVAVTGQVAGGCNVICFTTGRGSVSGFKPSPCIKIATNSEMYEHMKEDMDINCGEIVTGTETIEESGKRIFEHI
LAVASGDKTVSEIYDYGDNEFVPWQVGAVT

Sequences:

>Translated_510_residues
MSENSEATILLNANDNVAVARRSIPAGGATGRGDLAALELIGRGHKVALKLIKSGDEVLKYGQVIGIATQDIEAGRHVHL
HNLAMVPSEHAHQFSVDIEEKGMVPEAERRTFMGYDRGLGGVGTRNYIGVIASVNCSATVSRYIADYFNKQGGLDGFDNV
DGVVALTHGGGCALNTKSEGYRLLIRTIQGYARHPNFGGILLIGLGCETNQIAPILEHYKMEEGERLRTMTIQQHGGTRK
TIDAAITEIKAMLPMVNAARRTEQPLSKLKVALECGGSDGYSGISANPALGYASDLIVRNGGTTVLSETPEIYGAEHLLT
KRAVTPAVAEKLLQRIDWWRDYTARNGDELNNNPSHGNKLGGLTTILEKSLGAVAKGGSMPLKAVYEFAETVTEQGFVFM
DTPGYDPVAVTGQVAGGCNVICFTTGRGSVSGFKPSPCIKIATNSEMYEHMKEDMDINCGEIVTGTETIEESGKRIFEHI
LAVASGDKTVSEIYDYGDNEFVPWQVGAVT
>Mature_509_residues
SENSEATILLNANDNVAVARRSIPAGGATGRGDLAALELIGRGHKVALKLIKSGDEVLKYGQVIGIATQDIEAGRHVHLH
NLAMVPSEHAHQFSVDIEEKGMVPEAERRTFMGYDRGLGGVGTRNYIGVIASVNCSATVSRYIADYFNKQGGLDGFDNVD
GVVALTHGGGCALNTKSEGYRLLIRTIQGYARHPNFGGILLIGLGCETNQIAPILEHYKMEEGERLRTMTIQQHGGTRKT
IDAAITEIKAMLPMVNAARRTEQPLSKLKVALECGGSDGYSGISANPALGYASDLIVRNGGTTVLSETPEIYGAEHLLTK
RAVTPAVAEKLLQRIDWWRDYTARNGDELNNNPSHGNKLGGLTTILEKSLGAVAKGGSMPLKAVYEFAETVTEQGFVFMD
TPGYDPVAVTGQVAGGCNVICFTTGRGSVSGFKPSPCIKIATNSEMYEHMKEDMDINCGEIVTGTETIEESGKRIFEHIL
AVASGDKTVSEIYDYGDNEFVPWQVGAVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=481, Percent_Identity=38.045738045738, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789475, Length=497, Percent_Identity=35.8148893360161, Blast_Score=283, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 54711; Mature: 54579

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSEATILLNANDNVAVARRSIPAGGATGRGDLAALELIGRGHKVALKLIKSGDEVLK
CCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCHHHHH
YGQVIGIATQDIEAGRHVHLHNLAMVPSEHAHQFSVDIEEKGMVPEAERRTFMGYDRGLG
HCCEEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCHHHHHHCCCCCCCC
GVGTRNYIGVIASVNCSATVSRYIADYFNKQGGLDGFDNVDGVVALTHGGGCALNTKSEG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCCCC
YRLLIRTIQGYARHPNFGGILLIGLGCETNQIAPILEHYKMEEGERLRTMTIQQHGGTRK
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHH
TIDAAITEIKAMLPMVNAARRTEQPLSKLKVALECGGSDGYSGISANPALGYASDLIVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEEC
GGTTVLSETPEIYGAEHLLTKRAVTPAVAEKLLQRIDWWRDYTARNGDELNNNPSHGNKL
CCCEEECCCCCHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
GGLTTILEKSLGAVAKGGSMPLKAVYEFAETVTEQGFVFMDTPGYDPVAVTGQVAGGCNV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCEE
ICFTTGRGSVSGFKPSPCIKIATNSEMYEHMKEDMDINCGEIVTGTETIEESGKRIFEHI
EEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHH
LAVASGDKTVSEIYDYGDNEFVPWQVGAVT
HHHHCCCHHHHHHHHCCCCCCCCEECCCCC
>Mature Secondary Structure 
SENSEATILLNANDNVAVARRSIPAGGATGRGDLAALELIGRGHKVALKLIKSGDEVLK
CCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCHHHHH
YGQVIGIATQDIEAGRHVHLHNLAMVPSEHAHQFSVDIEEKGMVPEAERRTFMGYDRGLG
HCCEEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCHHHHHHCCCCCCCC
GVGTRNYIGVIASVNCSATVSRYIADYFNKQGGLDGFDNVDGVVALTHGGGCALNTKSEG
CCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCCCC
YRLLIRTIQGYARHPNFGGILLIGLGCETNQIAPILEHYKMEEGERLRTMTIQQHGGTRK
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHH
TIDAAITEIKAMLPMVNAARRTEQPLSKLKVALECGGSDGYSGISANPALGYASDLIVRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEEC
GGTTVLSETPEIYGAEHLLTKRAVTPAVAEKLLQRIDWWRDYTARNGDELNNNPSHGNKL
CCCEEECCCCCHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
GGLTTILEKSLGAVAKGGSMPLKAVYEFAETVTEQGFVFMDTPGYDPVAVTGQVAGGCNV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCEE
ICFTTGRGSVSGFKPSPCIKIATNSEMYEHMKEDMDINCGEIVTGTETIEESGKRIFEHI
EEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHH
LAVASGDKTVSEIYDYGDNEFVPWQVGAVT
HHHHCCCHHHHHHHHCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]