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The map label for this gene is leuD

Identifier: 159185383

GI number: 159185383

Start: 2791645

End: 2792250

Strand: Direct

Name: leuD

Synonym: Atu2790

Alternate gene names: 159185383

Gene position: 2791645-2792250 (Clockwise)

Preceding gene: 15890036

Following gene: 15890038

Centisome position: 98.24

GC content: 57.59

Gene sequence:

>606_bases
ATGGAAAAATTTACCAAGCTGACGGGCGTCGCCGCGCCCATGCCGGTCGTCAATATTGACACCGACATGATCATTCCGAA
AGATTATCTGAAGACCATCAAGCGCACCGGTCTCGGCAAGGGTCTTTTCGCCGAATCCCGTTATCTTGAAGACGGTTCGC
CCAATCCCGATTTCGTGCTGAACAAGCCCGCCTACCAGAACGCCCAGATTCTCGTTGCCGGTGACAATTTCGGCTGCGGC
TCGTCGCGCGAACATGCACCATGGGCACTGCTCGATTTCGGCATCCGTTGCGTCATCTCCACCAGCTTCGCCGACATCTT
CTACAACAACTGTTTCAAGAACGGCATCCTGCCGGTTGTCGTCAGCCCCGAGAACCTCGAAAAGCTGCTGGACGACGCCT
CGCGCGGCTCGAACGCCGTTCTCTCCATCGATCTGGAGCGCCAGGAAATCAGCGGTCCGGATGGTGGCACGATCACCTTC
GAGATCGACGAATTCAAGCGACACTGCATGCTGAACGGTCTCGACGATATCGGCCTGACACTGGAACATGCCGGCGCCAT
CGACACGTTCGAAAAGGCGAACGCCTCCGTCCGTCCCTGGGCCTGA

Upstream 100 bases:

>100_bases
CTTCTAAAGCGCCTGGTTTTGCAGTATTTCCCCAGAGGAAACATCCGGCAGGCTTTTGTCAGTGCCGGATTTTGTTCAAG
GGAAACACGGGAAGCGACAG

Downstream 100 bases:

>100_bases
TTACATTTCCGCTCTTCAAAAAAGCCGATCCCGTACCAAACCGTACCGGATCGGCTTTTGCTTTGCTTGAAAAGCCCTTG
AGGCGGAGATAAGAAGCCCT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEKFTKLTGVAAPMPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAESRYLEDGSPNPDFVLNKPAYQNAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSPENLEKLLDDASRGSNAVLSIDLERQEISGPDGGTITF
EIDEFKRHCMLNGLDDIGLTLEHAGAIDTFEKANASVRPWA

Sequences:

>Translated_201_residues
MEKFTKLTGVAAPMPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAESRYLEDGSPNPDFVLNKPAYQNAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSPENLEKLLDDASRGSNAVLSIDLERQEISGPDGGTITF
EIDEFKRHCMLNGLDDIGLTLEHAGAIDTFEKANASVRPWA
>Mature_201_residues
MEKFTKLTGVAAPMPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAESRYLEDGSPNPDFVLNKPAYQNAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSPENLEKLLDDASRGSNAVLSIDLERQEISGPDGGTITF
EIDEFKRHCMLNGLDDIGLTLEHAGAIDTFEKANASVRPWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=52.0833333333333, Blast_Score=206, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=51.5151515151515, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_AGRT5 (Q8UBR0)

Other databases:

- EMBL:   AE007869
- PIR:   AE2919
- PIR:   F97693
- RefSeq:   NP_355718.2
- ProteinModelPortal:   Q8UBR0
- SMR:   Q8UBR0
- STRING:   Q8UBR0
- GeneID:   1134828
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2790
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q8UBR0
- ProtClustDB:   PRK01641
- BioCyc:   ATUM176299-1:ATU2790-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21984; Mature: 21984

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTKLTGVAAPMPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAESRYLEDGSPNPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
NKPAYQNAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSPENLEKLLDDASRGSNAVLSIDLERQEISGPDGGTITFEIDEFKRHCMLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEHAGAIDTFEKANASVRPWA
EECCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MEKFTKLTGVAAPMPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAESRYLEDGSPNPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE
NKPAYQNAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSPENLEKLLDDASRGSNAVLSIDLERQEISGPDGGTITFEIDEFKRHCMLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEHAGAIDTFEKANASVRPWA
EECCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194