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The map label for this gene is amtB [H]

Identifier: 159185365

GI number: 159185365

Start: 2757775

End: 2758980

Strand: Direct

Name: amtB [H]

Synonym: Atu2758

Alternate gene names: 159185365

Gene position: 2757775-2758980 (Clockwise)

Preceding gene: 15890006

Following gene: 159185366

Centisome position: 97.05

GC content: 60.61

Gene sequence:

>1206_bases
ATGTATATCGCAACGATCCTCGTGCTGTTCATGATCATTCCTGGTCTGGCGCTGTTTTACGGCGGTCTGGTCCGCACCAA
GAACATGCTCTCCGTTCTCATGCAGTGCACGGTCATCGGCGCAGTCATGATGCTGGTGTGGGTCATCTACGGTTACTCCT
TCGCCTTCGGCGGCGGCACCGGTCCTTATTTCGGCGGCTTCGGCAAGCTGTTCCTGTCGGGCGTTTCGCTTGACAGCACG
TCTGCCACCTTCTCGCAGGGTGTCGTGATCTACGAATACACCTTCATCGCCTTCCAGATGACTTTTGCGGCCATCACGCC
GGCGCTGATCATCGGTGCTTTTGCAGAACGCGTGAAGTTCTCCGCCGTTATCCTTTTCACCATCCTCTGGGCCACCTTCG
TTTATTTCCCGATCGCGCACATGGTCTGGGACGCGAACGGCCTGATCTTCGGCATGGGCGCTCTCGACTTCGCCGGCGGC
ACCGTCGTTCACATCAATGCCGGTATCGCTGGCCTGATCGGCGCTATCATGGTTGGCAAACGCACCGGCTTCGGCAAGGA
CATGATGGCTCCCCACTCCATGACGCTGACGCTGGTTGGCGCCGCCATGCTGTGGTTCGGCTGGTTCGGCTTTAACGCCG
GCTCCAACCTCGAGGCATCGGGCGGTGCGGTTCTCGCAACCATGAACACCTTCCTCGCCACTGCTGCGGCCATCGTTTCC
TGGTCGCTGGTTGAGAGCTTCACCCGCGGCAAGGCTTCCATGCTGGGTGCTGCTTCGGGCATGATCGCCGGCCTCGTTGC
CGTCACGCCTGCTGCCGGCTCCGTCGGCCCGATGGGTGCGATCGTTCTCGGTCTCATCGTTTCGCCGATCTGCTACTTCT
TCGTCTCCGTGGTGAAGAACAAGTTCGGTTATGACGATACTGCCGACGTCTTCGGCGTCCACGGCATCGGTGGCATCATT
GGCGCCCTCGGCACCGGCATCTTCACCTCGCCGCTGCTTGGCGGCACCGGCAAGGCCGATTTCTCCATCTCCTCGCAGCT
CTGGACCCAGTTCGTTGCCGTCGCCATCACCATCGTCTGGTGCGCGGTCGTTTCGGCTATCCTCTACAAGATCGTCGACG
TGATCGTTGGTCTTCGGGTTCCGGTCGAAGCCGAACGCGAAGGTCTCGACCTCGCGACCCACGGCGAAGCCGCTTACCAC
TCCTGA

Upstream 100 bases:

>100_bases
AGCAACGGCCCTGATGGCCCCGGCCATCGCCTTTGCCCAGGATGCAGCGCCAGCAGTAGCGGCAGCGGCTCCTGTTCCGG
AAAAAGCCGATACCGCCTTC

Downstream 100 bases:

>100_bases
TATCAGGGAAAAGGGGTTCTCCATCCCCTCTCCCGCAAGAAGGCCCGCTCCGGCGGGCCTTTTTCTTTGCCGTAAATCCG
CGCTCGGCCTCAAAAAGCCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MYIATILVLFMIIPGLALFYGGLVRTKNMLSVLMQCTVIGAVMMLVWVIYGYSFAFGGGTGPYFGGFGKLFLSGVSLDST
SATFSQGVVIYEYTFIAFQMTFAAITPALIIGAFAERVKFSAVILFTILWATFVYFPIAHMVWDANGLIFGMGALDFAGG
TVVHINAGIAGLIGAIMVGKRTGFGKDMMAPHSMTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATMNTFLATAAAIVS
WSLVESFTRGKASMLGAASGMIAGLVAVTPAAGSVGPMGAIVLGLIVSPICYFFVSVVKNKFGYDDTADVFGVHGIGGII
GALGTGIFTSPLLGGTGKADFSISSQLWTQFVAVAITIVWCAVVSAILYKIVDVIVGLRVPVEAEREGLDLATHGEAAYH
S

Sequences:

>Translated_401_residues
MYIATILVLFMIIPGLALFYGGLVRTKNMLSVLMQCTVIGAVMMLVWVIYGYSFAFGGGTGPYFGGFGKLFLSGVSLDST
SATFSQGVVIYEYTFIAFQMTFAAITPALIIGAFAERVKFSAVILFTILWATFVYFPIAHMVWDANGLIFGMGALDFAGG
TVVHINAGIAGLIGAIMVGKRTGFGKDMMAPHSMTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATMNTFLATAAAIVS
WSLVESFTRGKASMLGAASGMIAGLVAVTPAAGSVGPMGAIVLGLIVSPICYFFVSVVKNKFGYDDTADVFGVHGIGGII
GALGTGIFTSPLLGGTGKADFSISSQLWTQFVAVAITIVWCAVVSAILYKIVDVIVGLRVPVEAEREGLDLATHGEAAYH
S
>Mature_401_residues
MYIATILVLFMIIPGLALFYGGLVRTKNMLSVLMQCTVIGAVMMLVWVIYGYSFAFGGGTGPYFGGFGKLFLSGVSLDST
SATFSQGVVIYEYTFIAFQMTFAAITPALIIGAFAERVKFSAVILFTILWATFVYFPIAHMVWDANGLIFGMGALDFAGG
TVVHINAGIAGLIGAIMVGKRTGFGKDMMAPHSMTLTLVGAAMLWFGWFGFNAGSNLEASGGAVLATMNTFLATAAAIVS
WSLVESFTRGKASMLGAASGMIAGLVAVTPAAGSVGPMGAIVLGLIVSPICYFFVSVVKNKFGYDDTADVFGVHGIGGII
GALGTGIFTSPLLGGTGKADFSISSQLWTQFVAVAITIVWCAVVSAILYKIVDVIVGLRVPVEAEREGLDLATHGEAAYH
S

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=402, Percent_Identity=52.7363184079602, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI133931100, Length=421, Percent_Identity=27.0783847980998, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=28.537170263789, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=427, Percent_Identity=28.3372365339578, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=28.7703016241299, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=413, Percent_Identity=35.1089588377724, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325396, Length=409, Percent_Identity=33.0073349633252, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=30.7506053268765, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI161078314, Length=407, Percent_Identity=26.2899262899263, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41863; Mature: 41863

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]