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The map label for this gene is leuC

Identifier: 159185339

GI number: 159185339

Start: 2693584

End: 2694993

Strand: Direct

Name: leuC

Synonym: Atu2709

Alternate gene names: 159185339

Gene position: 2693584-2694993 (Clockwise)

Preceding gene: 159185412

Following gene: 159185345

Centisome position: 94.79

GC content: 60.35

Gene sequence:

>1410_bases
ATGAGCGCACCCCGCACTTTATATGACAAGATCTGGGACGACCACGTCGTCAATCGTGACCCGGACGGAACCTGTCTTCT
CTACATCGACCGTCATCTCGTGCATGAGGTGACGAGCCCACAGGCCTTCGAAGGTTTGCGCATTGCCGGTAGACCCGTGC
ATTCGCCGACCCGCACGCTCGCCGTGGTCGACCATAACGTTCCGACCACAGCCGACCGGCTGGAAGGCATCAAGAACGAG
GAAAGCCGCATTCAGGTGGAAGCGCTTGCGCAGAACGCCAAGGAGTTCGGTGTCGAATATTATTCCGAGCGCGACAAGCG
CCAGGGCATCGTCCACATCGTCGGCCCCGAGCAGGGCTTCACCCTGCCCGGCATGACGATCGTCTGCGGCGATAGCCACA
CCTCCACCCACGGCGCTTTCGGCGCTCTGGCGCACGGTATCGGCACGTCGGAAGTGGAGCATGTTCTGGCCACCCAGACG
CTGATCCAGAAAAAGGCCAAGAACATGCTGGTGCGTGTCGATGGCAAGCTTCCGGAAAGCGTGACCGCCAAGGACATCAT
CCTTGCCATTATCGGCGAGATCGGCACGGCCGGCGGCACCGGCCATGTGATCGAATTTGCTGGCGAGGCGATCCGCTCGC
TCTCCATGGAAGGGCGCATGACGGTCTGCAACATGACCATCGAGGGCGGCGCCCGCGCCGGTCTGATCGCACCCGATGAA
ACGACTTTCGATTACATCAAGGACAAACCGCGTGCGCCGAAGGGTGAGACGCTGGAACAGGCCATCGCATATTGGAAGAC
GCTGAAATCCGATGAGGGCGCCCATTACGACAAGGTTGTCATCCTCGATGCAGCCAATCTGCCGCCAATTGTCTCCTGGG
GTTCATCTCCGGAGGATGTGACCTCCGTTGAAGGCATCGTTCCCAATCCCGATGATATCGAGGAAGAAAACAAGCGCACT
TCCAAATGGCGCGCGCTTGACTATATGGGCCTGAAACCCGGCACGCGCATCACCGATATCGCCATCGACCGTGTCTTCAT
CGGCTCCTGCACCAATGGCCGCATCGAGGATCTGCGGGCGGCGGCCAAGATCGTCGATGGCCGCAAGGTGGCTTCCACCG
TCTCGGCCATGATCGTGCCCGGCTCCGGACTCGTCAAGGAACAGGCGGAAAAAGAAGGTCTGGACAAGATTTTCCTCGAC
GCCGGTTTCGAATGGCGCGAACCGGGCTGCTCTATGTGCCTTGCCATGAATGACGACCGCCTGAAGCCCGGCGAACGCTG
TGCCTCCACCTCGAACCGCAACTTCGAGGGCCGTCAGGGCTATAAGAGCCGAACCCACCTCGTCTCGCCAGCCATGGCTG
CGGCTGCGGCAATTGCCGGTCATTTCGTCGACGTGCGCGAGTGGCAATAA

Upstream 100 bases:

>100_bases
CCGGGGCGCTCAACAGCCGACTTAGGCTCGCCTGACAGCACCCGCTGTCCACCGCCTTTCTGAACCCTGATTACCCTTTT
CCATGACAAGGCATAGAGCC

Downstream 100 bases:

>100_bases
TTCTAACAGATGCCAGACAACAAAAAACCCCGGAGCGATCCGGGGTTTTTCTTTGGCTCTATTCCGAAATGATCTTGACC
CGCTGGCCGGGCTGAAGCGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIWDDHVVNRDPDGTCLLYIDRHLVHEVTSPQAFEGLRIAGRPVHSPTRTLAVVDHNVPTTADRLEGIKNE
ESRIQVEALAQNAKEFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPESVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
TTFDYIKDKPRAPKGETLEQAIAYWKTLKSDEGAHYDKVVILDAANLPPIVSWGSSPEDVTSVEGIVPNPDDIEEENKRT
SKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAAAKIVDGRKVASTVSAMIVPGSGLVKEQAEKEGLDKIFLD
AGFEWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGYKSRTHLVSPAMAAAAAIAGHFVDVREWQ

Sequences:

>Translated_469_residues
MSAPRTLYDKIWDDHVVNRDPDGTCLLYIDRHLVHEVTSPQAFEGLRIAGRPVHSPTRTLAVVDHNVPTTADRLEGIKNE
ESRIQVEALAQNAKEFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPESVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
TTFDYIKDKPRAPKGETLEQAIAYWKTLKSDEGAHYDKVVILDAANLPPIVSWGSSPEDVTSVEGIVPNPDDIEEENKRT
SKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAAAKIVDGRKVASTVSAMIVPGSGLVKEQAEKEGLDKIFLD
AGFEWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGYKSRTHLVSPAMAAAAAIAGHFVDVREWQ
>Mature_468_residues
SAPRTLYDKIWDDHVVNRDPDGTCLLYIDRHLVHEVTSPQAFEGLRIAGRPVHSPTRTLAVVDHNVPTTADRLEGIKNEE
SRIQVEALAQNAKEFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMLVRVDGKLPESVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDET
TFDYIKDKPRAPKGETLEQAIAYWKTLKSDEGAHYDKVVILDAANLPPIVSWGSSPEDVTSVEGIVPNPDDIEEENKRTS
KWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRAAAKIVDGRKVASTVSAMIVPGSGLVKEQAEKEGLDKIFLDA
GFEWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGYKSRTHLVSPAMAAAAAIAGHFVDVREWQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=384, Percent_Identity=28.3854166666667, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI8659555, Length=450, Percent_Identity=25.3333333333333, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=66.2337662337662, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=26.7409470752089, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=25.7703081232493, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI2367097, Length=109, Percent_Identity=34.8623853211009, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=383, Percent_Identity=30.5483028720627, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=383, Percent_Identity=30.5483028720627, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=301, Percent_Identity=31.2292358803987, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=399, Percent_Identity=26.0651629072682, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=65.0423728813559, Blast_Score=615, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320440, Length=462, Percent_Identity=27.7056277056277, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=30.1939058171745, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=388, Percent_Identity=27.5773195876289, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365315, Length=481, Percent_Identity=26.1954261954262, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17864292, Length=481, Percent_Identity=26.1954261954262, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.4869109947644, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=28.1767955801105, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.0341207349081, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=25.5936675461741, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_AGRT5 (Q8UBY9)

Other databases:

- EMBL:   AE007869
- PIR:   AD2909
- PIR:   C97684
- RefSeq:   NP_355643.2
- ProteinModelPortal:   Q8UBY9
- SMR:   Q8UBY9
- STRING:   Q8UBY9
- GeneID:   1134747
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2709
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q8UBY9
- ProtClustDB:   PRK05478
- BioCyc:   ATUM176299-1:ATU2709-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51049; Mature: 50918

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIWDDHVVNRDPDGTCLLYIDRHLVHEVTSPQAFEGLRIAGRPVHSPTRTL
CCCCHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHCCCEECCCCCCCCCCEE
AVVDHNVPTTADRLEGIKNEESRIQVEALAQNAKEFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPES
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFDYIKDKPRAPKGETLEQAIAYWKTLKSDEGAHYDKVVILDAANLPPIVSWGSSPEDV
CHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCCH
TSVEGIVPNPDDIEEENKRTSKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRA
HHCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKIVDGRKVASTVSAMIVPGSGLVKEQAEKEGLDKIFLDAGFEWREPGCSMCLAMNDDR
HHHHHCCHHHHHHHHEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGYKSRTHLVSPAMAAAAAIAGHFVDVREWQ
CCCHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
SAPRTLYDKIWDDHVVNRDPDGTCLLYIDRHLVHEVTSPQAFEGLRIAGRPVHSPTRTL
CCCHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHCCCEECCCCCCCCCCEE
AVVDHNVPTTADRLEGIKNEESRIQVEALAQNAKEFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPES
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTVCNMTIEGGARAGLIAPDE
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFDYIKDKPRAPKGETLEQAIAYWKTLKSDEGAHYDKVVILDAANLPPIVSWGSSPEDV
CHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCCCCH
TSVEGIVPNPDDIEEENKRTSKWRALDYMGLKPGTRITDIAIDRVFIGSCTNGRIEDLRA
HHCCCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKIVDGRKVASTVSAMIVPGSGLVKEQAEKEGLDKIFLDAGFEWREPGCSMCLAMNDDR
HHHHHCCHHHHHHHHEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGYKSRTHLVSPAMAAAAAIAGHFVDVREWQ
CCCHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194