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The map label for this gene is argG [H]

Identifier: 159185316

GI number: 159185316

Start: 2651235

End: 2652458

Strand: Reverse

Name: argG [H]

Synonym: Atu2667

Alternate gene names: 159185316

Gene position: 2652458-2651235 (Counterclockwise)

Preceding gene: 15889926

Following gene: 159185314

Centisome position: 93.34

GC content: 59.48

Gene sequence:

>1224_bases
ATGGCACTCCCGAAAGACGTTAAGAAAGTCGTTCTCGCCTATTCCGGCGGCCTCGACACCTCGATCATCCTGAAATGGCT
TCAGACGGAACTCGGCGCCGAGGTCGTGACCTTCACCGCCGATCTCGGCCAGGGCGAAGAGCTTGAGCCGGCACGCAAGA
AGGCTGAGATGCTGGGCATCAAGGAAATCTTTGTCGAGGACGTTCGCGAAGAGTTCGTGCGTGATTTCGTGTTTCCGATG
TTCCGCGCCAATGCCGTTTATGAAGGCGTCTATCTGCTCGGCACGTCGATTGCCCGTCCGCTGATTTCCAAGCACCTGAT
CGAGATCGCCAAGAAGACCGGCGCTGACGCCATCGCCCACGGCGCCACCGGCAAGGGCAACGACCAGGTCCGCTTCGAGC
TTTCGGCCTATGCGTTGAACCCTGACATCAAGATCATTGCGCCCTGGCGCGACTGGACGTTCAAGAGCCGTACCGACCTC
CTGAACTTCGCCGAACAGCACCAGATCCCGGTCGCCAAGGACAAGAAGGGTGAAGCGCCTTTCTCCGTTGACGCCAACCT
CTTGCACTCCTCCTCCGAGGGCAAGGTGCTGGAAGACCCGGCGGTGGAAGCACCTGAATATGTGCATATGCGCACCATTT
CCCCGGAAGCGGCTCCCGACAAGGCGACCGTCATCAAGGTCGGTTTCCGCAAGGGCGATGCCTGCTCCATCAACGGTGTT
GAAATGTCGCCCGCGACGCTTCTTGCCACGCTCAACAACTACGGTCGCGAAAACGGCATCGGCCGCCTCGATCTGGTCGA
GAACCGTTTCGTCGGCATGAAGTCACGCGGCGTTTACGAGACGCCCGGCGGCACGATCCTGCTGGCAGCGCACCGCGCCA
TCGAATCCATCACACTCGACCGGGGTGCCGCGCATCTCAAGGACGATCTGATGCCGCGTTACGCTGAGCTTATCTATTAC
GGCTTCTGGTTTTCGCCGGAGCGCGAAATGCTGCAGGCCCTGATCGACAAGAGCCAGGAACATGTCGAAGGCGAAGTGAC
GCTGAAGCTCTACAAGGGCAATGTCATGGTAACCGGCCGCGAGAGCGAGAAGTCGCTGTATTCCGACGCGCTGGTGACCT
TCGAGGACGACCACGGCGCTTACGACCAGAAGGATGCTGCTGGCTTCATCAAGCTCAATGCGCTGCGTCTTCGCACGCTC
GCCAAGCGTAACCTCAACAAGTAA

Upstream 100 bases:

>100_bases
GGAAAAGCGCTTCACACATTTCGGGTTGCACTGTAAAAGTGCCGAAATCCCAAGGAAATGGCGGTGCCCCGATGCCGTCA
TCATGCAGATGGACATCATT

Downstream 100 bases:

>100_bases
TTCAGCTGACGTAAAGATTATGAAACCCGCGGGCGGTGACGCCGGCGGGTTTTTTCATGTCTTGCTTCTGGAGACGATGC
GGAAGCCGATGTAGCTGGCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase 2 [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MALPKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFVEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIEIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWTFKSRTDL
LNFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAVEAPEYVHMRTISPEAAPDKATVIKVGFRKGDACSINGV
EMSPATLLATLNNYGRENGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDDLMPRYAELIYY
GFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDALVTFEDDHGAYDQKDAAGFIKLNALRLRTL
AKRNLNK

Sequences:

>Translated_407_residues
MALPKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFVEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIEIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWTFKSRTDL
LNFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAVEAPEYVHMRTISPEAAPDKATVIKVGFRKGDACSINGV
EMSPATLLATLNNYGRENGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDDLMPRYAELIYY
GFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDALVTFEDDHGAYDQKDAAGFIKLNALRLRTL
AKRNLNK
>Mature_406_residues
ALPKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFVEDVREEFVRDFVFPMF
RANAVYEGVYLLGTSIARPLISKHLIEIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWTFKSRTDLL
NFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPAVEAPEYVHMRTISPEAAPDKATVIKVGFRKGDACSINGVE
MSPATLLATLNNYGRENGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDDLMPRYAELIYYG
FWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGRESEKSLYSDALVTFEDDHGAYDQKDAAGFIKLNALRLRTLA
KRNLNK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=48.9949748743719, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=48.9949748743719, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1789563, Length=405, Percent_Identity=27.4074074074074, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=42.5742574257426, Blast_Score=329, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21358151, Length=409, Percent_Identity=47.921760391198, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45131; Mature: 45000

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIFVEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIEIAKKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWTFKSRTDLLNFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAVEAPEYVHMRTISPEAAPDKATVIKVGFRKGDACSINGV
CCCCHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
EMSPATLLATLNNYGRENGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDDLMPRYAELIYYGFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCHHHCCCEEEEEEEECCEEEECC
ESEKSLYSDALVTFEDDHGAYDQKDAAGFIKLNALRLRTLAKRNLNK
CHHHHHHHCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALPKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIFVEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIEIAKKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWTFKSRTDLLNFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAVEAPEYVHMRTISPEAAPDKATVIKVGFRKGDACSINGV
CCCCHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
EMSPATLLATLNNYGRENGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDDLMPRYAELIYYGFWFSPEREMLQALIDKSQEHVEGEVTLKLYKGNVMVTGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHCCHHHCCCEEEEEEEECCEEEECC
ESEKSLYSDALVTFEDDHGAYDQKDAAGFIKLNALRLRTLAKRNLNK
CHHHHHHHCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA