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The map label for this gene is cls [H]

Identifier: 159185236

GI number: 159185236

Start: 2454549

End: 2456009

Strand: Reverse

Name: cls [H]

Synonym: Atu2486

Alternate gene names: 159185236

Gene position: 2456009-2454549 (Counterclockwise)

Preceding gene: 159185238

Following gene: 159185235

Centisome position: 86.43

GC content: 60.1

Gene sequence:

>1461_bases
ATGCTCGATCTCGTCATTGCCTATTGGCCGCATATCCTTGCCGTTCTCTCCATCTTGATGGGTACCGTGGCGGCCGTGCA
TGCCACGATGACGAAGGAGGAGGTGCGTTCCGCGCTGGGCTGGGTGGGCGTCATCGTGCTGTCGCCGATCGTCGGCGCGG
TCATCTACGCCATCGCCGGCATCAACCGCATTCGCCGGTCGAACATCACGCAGCAGCGGTCCTTCATGCTGGACGAGATC
ATGGATCGCGTGGCGCGCCTCGATGCCAGCAACGATATGATCGCGACGCGTTTCGGCCGCCGTTTCGAGGCGATGAAGAC
GCTTGGCGACCGGGTGACGCGCCACGCGCTGACGACCGGTAACGGCATCGAGCCGCTGGTGAGCGGGGATGTCGCCTATG
CCGCCATGCTGGAGGCGATCGGGGAGGCGAAGCGGTCGATCATTCTCGAAACATATATTTTCGATAATGACCGGATCGGC
GCCCGTTTCGTCGCGGCGCTTGAAAGGGCGAAGCTGCGTGGCGTGGAGGTGAGGGTGCTCATCGATGCGGTCGGCGCCCG
TTATTCGGTTCCGAGCATCCTGCCGACACTCAGGGATAAGGACATCGTTGCCGATGTCTTCAACGGCAATGTCATCATGG
GGCTGCGGCTGCCCTATGCCAATCTGCGTACCCACCGCAAGATCCTCGTGGTGGACGGACGTATCGCCTTCAGCGGCGGC
ATGAATATCCGCGAGGGTTTCACCCTGGAATTCGGTGGCGAAAGCCAGTCGCACGACACCCATTTCAAGATTACCGGGCC
GGTGGTCTCCGATTTTTTCTCCATCGCTGCGGAGGACTGGCGCTTCACGACGGGGGAGGTTCTCGACGCACCGGTCTGGG
ATATCGCCATTCCCGATGGCGTGCCGGGGTCGCAAATCGTTGCCCGCGTCTGCTCCTCGGGGCCGGACAAAAGCATCGAG
ACCAGTCACAAGATGCTGATGGGGGCCTTTTCGGTGGCGCGTTCCTCCATTCTCATCATGTCGCCCTATTTTCTCCCCGA
TCGGGAGCTTATTTCGGCGCTCATTACCGCAGCAAGGCGGGGGTGCGTGGTGGATATCATCGTGCCGAAAAGCAACAATC
TGGTGCTTGTCGACAGGGCGATGACGGCGCAGTTCGACCAGATGCTGAAGAATTATTGCCGCATCTGGCGGGCGACCGGG
GCTTTCAATCATTCCAAGCTTCTGGTGATCGACGGGCGCTGGAGCTATATTGGCTCCTCCAATCTCGATCCGCGATCGCT
GCGGTTGAACTTCGAGATCGATCTCGAAGTCATGGACGAAGAATTTGCCGGGGCGATCGGAGATCGTATCCGGAATGCGC
GTGCAACTGCCTTGCCCGTCCGGCTCAGCGAACTGAATGCTCGGCCATTCGTTGTCCGTTTTGTGGAGCGTGTGCTGTGG
CTCGCTTCACCCTATCTTTAG

Upstream 100 bases:

>100_bases
ATGGTCTGTCCCGCAAAAGCATGAGCGGGGCCGGGCGTTCTTCCGTTGCAGGGGCGTTACCTGTCATTGCGGGATCGGCT
GAGTGCTCTATAGTCTTATC

Downstream 100 bases:

>100_bases
AGTGCGGTGCGCCCGAAAGGACGCCCCGCGAGACCGGAGGCATAAACGCGTGAAAAAGAACGGTTTGCTGGATGATGAAA
AAACGGCTTTGACGAAAAAC

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLDLVIAYWPHILAVLSILMGTVAAVHATMTKEEVRSALGWVGVIVLSPIVGAVIYAIAGINRIRRSNITQQRSFMLDEI
MDRVARLDASNDMIATRFGRRFEAMKTLGDRVTRHALTTGNGIEPLVSGDVAYAAMLEAIGEAKRSIILETYIFDNDRIG
ARFVAALERAKLRGVEVRVLIDAVGARYSVPSILPTLRDKDIVADVFNGNVIMGLRLPYANLRTHRKILVVDGRIAFSGG
MNIREGFTLEFGGESQSHDTHFKITGPVVSDFFSIAAEDWRFTTGEVLDAPVWDIAIPDGVPGSQIVARVCSSGPDKSIE
TSHKMLMGAFSVARSSILIMSPYFLPDRELISALITAARRGCVVDIIVPKSNNLVLVDRAMTAQFDQMLKNYCRIWRATG
AFNHSKLLVIDGRWSYIGSSNLDPRSLRLNFEIDLEVMDEEFAGAIGDRIRNARATALPVRLSELNARPFVVRFVERVLW
LASPYL

Sequences:

>Translated_486_residues
MLDLVIAYWPHILAVLSILMGTVAAVHATMTKEEVRSALGWVGVIVLSPIVGAVIYAIAGINRIRRSNITQQRSFMLDEI
MDRVARLDASNDMIATRFGRRFEAMKTLGDRVTRHALTTGNGIEPLVSGDVAYAAMLEAIGEAKRSIILETYIFDNDRIG
ARFVAALERAKLRGVEVRVLIDAVGARYSVPSILPTLRDKDIVADVFNGNVIMGLRLPYANLRTHRKILVVDGRIAFSGG
MNIREGFTLEFGGESQSHDTHFKITGPVVSDFFSIAAEDWRFTTGEVLDAPVWDIAIPDGVPGSQIVARVCSSGPDKSIE
TSHKMLMGAFSVARSSILIMSPYFLPDRELISALITAARRGCVVDIIVPKSNNLVLVDRAMTAQFDQMLKNYCRIWRATG
AFNHSKLLVIDGRWSYIGSSNLDPRSLRLNFEIDLEVMDEEFAGAIGDRIRNARATALPVRLSELNARPFVVRFVERVLW
LASPYL
>Mature_486_residues
MLDLVIAYWPHILAVLSILMGTVAAVHATMTKEEVRSALGWVGVIVLSPIVGAVIYAIAGINRIRRSNITQQRSFMLDEI
MDRVARLDASNDMIATRFGRRFEAMKTLGDRVTRHALTTGNGIEPLVSGDVAYAAMLEAIGEAKRSIILETYIFDNDRIG
ARFVAALERAKLRGVEVRVLIDAVGARYSVPSILPTLRDKDIVADVFNGNVIMGLRLPYANLRTHRKILVVDGRIAFSGG
MNIREGFTLEFGGESQSHDTHFKITGPVVSDFFSIAAEDWRFTTGEVLDAPVWDIAIPDGVPGSQIVARVCSSGPDKSIE
TSHKMLMGAFSVARSSILIMSPYFLPDRELISALITAARRGCVVDIIVPKSNNLVLVDRAMTAQFDQMLKNYCRIWRATG
AFNHSKLLVIDGRWSYIGSSNLDPRSLRLNFEIDLEVMDEEFAGAIGDRIRNARATALPVRLSELNARPFVVRFVERVLW
LASPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=485, Percent_Identity=28.659793814433, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787007, Length=343, Percent_Identity=29.7376093294461, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53779; Mature: 53779

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLVIAYWPHILAVLSILMGTVAAVHATMTKEEVRSALGWVGVIVLSPIVGAVIYAIAG
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRIRRSNITQQRSFMLDEIMDRVARLDASNDMIATRFGRRFEAMKTLGDRVTRHALTTG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NGIEPLVSGDVAYAAMLEAIGEAKRSIILETYIFDNDRIGARFVAALERAKLRGVEVRVL
CCCCCHHCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
IDAVGARYSVPSILPTLRDKDIVADVFNGNVIMGLRLPYANLRTHRKILVVDGRIAFSGG
EEHHCCCCCCHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHCCCCEEEEEECEEEEECC
MNIREGFTLEFGGESQSHDTHFKITGPVVSDFFSIAAEDWRFTTGEVLDAPVWDIAIPDG
CCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VPGSQIVARVCSSGPDKSIETSHKMLMGAFSVARSSILIMSPYFLPDRELISALITAARR
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCC
GCVVDIIVPKSNNLVLVDRAMTAQFDQMLKNYCRIWRATGAFNHSKLLVIDGRWSYIGSS
CCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCC
NLDPRSLRLNFEIDLEVMDEEFAGAIGDRIRNARATALPVRLSELNARPFVVRFVERVLW
CCCCCEEEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHH
LASPYL
HHCCCC
>Mature Secondary Structure
MLDLVIAYWPHILAVLSILMGTVAAVHATMTKEEVRSALGWVGVIVLSPIVGAVIYAIAG
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRIRRSNITQQRSFMLDEIMDRVARLDASNDMIATRFGRRFEAMKTLGDRVTRHALTTG
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NGIEPLVSGDVAYAAMLEAIGEAKRSIILETYIFDNDRIGARFVAALERAKLRGVEVRVL
CCCCCHHCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEE
IDAVGARYSVPSILPTLRDKDIVADVFNGNVIMGLRLPYANLRTHRKILVVDGRIAFSGG
EEHHCCCCCCHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHCCCCEEEEEECEEEEECC
MNIREGFTLEFGGESQSHDTHFKITGPVVSDFFSIAAEDWRFTTGEVLDAPVWDIAIPDG
CCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VPGSQIVARVCSSGPDKSIETSHKMLMGAFSVARSSILIMSPYFLPDRELISALITAARR
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCC
GCVVDIIVPKSNNLVLVDRAMTAQFDQMLKNYCRIWRATGAFNHSKLLVIDGRWSYIGSS
CCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCC
NLDPRSLRLNFEIDLEVMDEEFAGAIGDRIRNARATALPVRLSELNARPFVVRFVERVLW
CCCCCEEEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEHHHCCCCHHHHHHHHHHHH
LASPYL
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA