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The map label for this gene is ureF

Identifier: 159185194

GI number: 159185194

Start: 2370167

End: 2370799

Strand: Reverse

Name: ureF

Synonym: Atu2397

Alternate gene names: 159185194

Gene position: 2370799-2370167 (Counterclockwise)

Preceding gene: 159185195

Following gene: 159185193

Centisome position: 83.43

GC content: 64.3

Gene sequence:

>633_bases
ATGGCCTGGCTGTCGCCGGCCTTTCCGGTTGGCGGTTTTTCCTATTCCGGCGGGCTGGAAAAGGCGGTCGAGGATGGGCG
CGTGTGCGATGCCGCCGGGTTGGGCGGCTGGGTGGAAACGCTGCTGCGCCATGGCAGCCTGTGGAATGATGCGGTGTTTC
TGGCGCATGCCTGGCGAAGCTCGCAGGATACCACGGCGCTCAGCGAAACGGCCAATCTCGGCCGCGCGCTGGCCGGTTCG
GCGGAACGTTATCGTGAGACCGTGCTTCTCGGTGATGCCTTCATTGCCGCTGCCAGCGCATGGCCGCATGCGGTTCTGGA
GCTATTGCCGAAGGAGGCTCCGTATCCCGTCGCCATAGGTGCTGTGGCGGCCGGACATGGAGTGCCATTGCGCGAGACGC
TTGCCGCTTTTCTGCATGCCGGTGTTTCGCAGATCGTTTCGGCCGGCATTCGTCTTGGCGTCGCGGGGCAAAAGGATGGC
GTCGCCATTCTCGCCGCAAGCGAGGCGGTGATCGAGGAAATTGCGGCGCGGGCGGCGTTATCAACACTCGATGATCTCGG
CAGCGCCACCGTTATCGCCGATACGGCGGCGATGCGTCATGAAACCCAGGGCACGCGGCTTTTCCGCTCCTGA

Upstream 100 bases:

>100_bases
CCATGGCGGCCATTCCCATGATCACGGGCAAGGCCACCACCATCATGACCACGGCTGAGGCGTGAGCGCGGAAAGCGGCG
TTGCCGCGCTGCTGCGGCTG

Downstream 100 bases:

>100_bases
CGGCGGCCCAATAAAGATCGATGGAGCGAGCGGGCGCTCCGTGTTTCTTCTCCCCGCCGGGGAGAAGTCCGCGGTAGCGG
GATGAAGGGGGCGAGGGTGC

Product: urease accessory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MAWLSPAFPVGGFSYSGGLEKAVEDGRVCDAAGLGGWVETLLRHGSLWNDAVFLAHAWRSSQDTTALSETANLGRALAGS
AERYRETVLLGDAFIAAASAWPHAVLELLPKEAPYPVAIGAVAAGHGVPLRETLAAFLHAGVSQIVSAGIRLGVAGQKDG
VAILAASEAVIEEIAARAALSTLDDLGSATVIADTAAMRHETQGTRLFRS

Sequences:

>Translated_210_residues
MAWLSPAFPVGGFSYSGGLEKAVEDGRVCDAAGLGGWVETLLRHGSLWNDAVFLAHAWRSSQDTTALSETANLGRALAGS
AERYRETVLLGDAFIAAASAWPHAVLELLPKEAPYPVAIGAVAAGHGVPLRETLAAFLHAGVSQIVSAGIRLGVAGQKDG
VAILAASEAVIEEIAARAALSTLDDLGSATVIADTAAMRHETQGTRLFRS
>Mature_209_residues
AWLSPAFPVGGFSYSGGLEKAVEDGRVCDAAGLGGWVETLLRHGSLWNDAVFLAHAWRSSQDTTALSETANLGRALAGSA
ERYRETVLLGDAFIAAASAWPHAVLELLPKEAPYPVAIGAVAAGHGVPLRETLAAFLHAGVSQIVSAGIRLGVAGQKDGV
AILAASEAVIEEIAARAALSTLDDLGSATVIADTAAMRHETQGTRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_AGRT5 (A9CHW6)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_355349.2
- STRING:   A9CHW6
- GeneID:   1134435
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2397
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   WLSPAFP
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 21699; Mature: 21568

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWLSPAFPVGGFSYSGGLEKAVEDGRVCDAAGLGGWVETLLRHGSLWNDAVFLAHAWRS
CCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
SQDTTALSETANLGRALAGSAERYRETVLLGDAFIAAASAWPHAVLELLPKEAPYPVAIG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEH
AVAAGHGVPLRETLAAFLHAGVSQIVSAGIRLGVAGQKDGVAILAASEAVIEEIAARAAL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
STLDDLGSATVIADTAAMRHETQGTRLFRS
HHHHHCCCCHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
AWLSPAFPVGGFSYSGGLEKAVEDGRVCDAAGLGGWVETLLRHGSLWNDAVFLAHAWRS
CCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
SQDTTALSETANLGRALAGSAERYRETVLLGDAFIAAASAWPHAVLELLPKEAPYPVAIG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEH
AVAAGHGVPLRETLAAFLHAGVSQIVSAGIRLGVAGQKDGVAILAASEAVIEEIAARAAL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
STLDDLGSATVIADTAAMRHETQGTRLFRS
HHHHHCCCCHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194