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The map label for this gene is papS [H]

Identifier: 159185123

GI number: 159185123

Start: 2221609

End: 2222865

Strand: Reverse

Name: papS [H]

Synonym: Atu2250

Alternate gene names: 159185123

Gene position: 2222865-2221609 (Counterclockwise)

Preceding gene: 15889527

Following gene: 159185121

Centisome position: 78.23

GC content: 61.02

Gene sequence:

>1257_bases
ATGAATAGCCTTGCCGGGCAGGACTGGTTCGAAAAACCGGCGCTCAAGCGTATCTTCGCGCTTCTGAATGCCGATGGCGG
CGAGGTGCGGATCGTCGGCGGCGCGGTGCGCAATGCGCTGATGGATTTGCCGGTTGTCGATGTCGACATGGCGACGACGG
TGACCCCTAATGTGGTTGTTGAGCGGGCGAAGGCCGCGGGCATCAAGTCGGTGCCAACAGGCATCGAACACGGCACGGTG
ACGCTGGTGATCGATGGCGAGGGTTTCGAGGTGACGACGTTGCGCCGCGATGTCGAGACAAATGGCCGCCATGCCCAGGT
GGCCTTCGGCACGGACTGGCAGACCGATGCCGAGCGGCGGGATCTCACCATCAACGCCCTTTACGCTAACGAAAAGGGTG
AGATCATCGACCTTGTCGATGGTCTAGCGGATGTGGAAACCCGCACGGTGCGCTTCATCGGGGATGCGGCGATGCGGATT
TCGGAAGATTATCTTCGAATCCTGCGGTTTTTTCGCTTTTTCGCCCATTATGGTTCAGGCCGCCCTGATGCCGACGGTTT
GCGGGCGAGTGCGCGGGCCAAGGACAAGCTTGGAACGCTTTCGGCTGAGCGGGTGTGGAGCGAAACGAAGAAACTGATTT
CTGCCCGCGACCCATCCCGCGCACTCCTGTGGATGCGGCAATCGGGTGTTCTCTCGGAAATTCTTCCCGAGACGGAAAAA
TGGGGCATTGATGCCATTCACGGGCTGGTGGCGACGGAGCAGGCGCTTGGTTGGGCCGCTGATCCGATGCTGCGGCTTGC
CGCCATCGTTCCGCCCGACAGGGAGAGGCTCGCTGCGCTTGCAGCCCGCCTCAGGCTGTCAAAGGCCGAGGCGGCTTATC
TTACGCATTGGGCTTCTGCCCCTGCCGTCGATCCGGAGATGAAAGAAACGGCATTGGACCGCCTGCTTTACCGGCAGGGT
GTCGAGGCCGTGAAGACACGATTGAAACTTGCGCTTGCTTCGGCTCGCGCCGATCTCTCAGCCGGCGATACGGCCATGCA
CAAAGTCGCGCGCCTTTCGACGTTGCTGACGAGGGCAGAGAAGTTCCGCAAGCCGGGTTTTCCGCTGAGCGGTGCCGATG
TGATGGCCGCCGGCGTCGAGGCTGGTCCGAAGGTAGGCGAGGTGCTGAAAAGCCTCGAAGAAAAGTGGATCGACGTCAAT
TTCTCACTCGACCGGGCAGCGCTGACCGCCCGGCTGAAGGATATGCTCGAGAATTGA

Upstream 100 bases:

>100_bases
AGGGGAAGGAGCGGTTTTTCTACGAAATGCCCTATGGCGAACGTTACATTTGGGGCATTACCGCGGGCATCGTACGAACG
ATTTACGAGAGGTTTTATAC

Downstream 100 bases:

>100_bases
AGTCGCGGCGAGGGCTGCTTTGCGGCTCAGGCCGCATTTGCCTCGTCGCGGATGCGGGATTTGATGTTTTCGATCATGTT
TTCGCGGATGATGGTTTCAC

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNSLAGQDWFEKPALKRIFALLNADGGEVRIVGGAVRNALMDLPVVDVDMATTVTPNVVVERAKAAGIKSVPTGIEHGTV
TLVIDGEGFEVTTLRRDVETNGRHAQVAFGTDWQTDAERRDLTINALYANEKGEIIDLVDGLADVETRTVRFIGDAAMRI
SEDYLRILRFFRFFAHYGSGRPDADGLRASARAKDKLGTLSAERVWSETKKLISARDPSRALLWMRQSGVLSEILPETEK
WGIDAIHGLVATEQALGWAADPMLRLAAIVPPDRERLAALAARLRLSKAEAAYLTHWASAPAVDPEMKETALDRLLYRQG
VEAVKTRLKLALASARADLSAGDTAMHKVARLSTLLTRAEKFRKPGFPLSGADVMAAGVEAGPKVGEVLKSLEEKWIDVN
FSLDRAALTARLKDMLEN

Sequences:

>Translated_418_residues
MNSLAGQDWFEKPALKRIFALLNADGGEVRIVGGAVRNALMDLPVVDVDMATTVTPNVVVERAKAAGIKSVPTGIEHGTV
TLVIDGEGFEVTTLRRDVETNGRHAQVAFGTDWQTDAERRDLTINALYANEKGEIIDLVDGLADVETRTVRFIGDAAMRI
SEDYLRILRFFRFFAHYGSGRPDADGLRASARAKDKLGTLSAERVWSETKKLISARDPSRALLWMRQSGVLSEILPETEK
WGIDAIHGLVATEQALGWAADPMLRLAAIVPPDRERLAALAARLRLSKAEAAYLTHWASAPAVDPEMKETALDRLLYRQG
VEAVKTRLKLALASARADLSAGDTAMHKVARLSTLLTRAEKFRKPGFPLSGADVMAAGVEAGPKVGEVLKSLEEKWIDVN
FSLDRAALTARLKDMLEN
>Mature_418_residues
MNSLAGQDWFEKPALKRIFALLNADGGEVRIVGGAVRNALMDLPVVDVDMATTVTPNVVVERAKAAGIKSVPTGIEHGTV
TLVIDGEGFEVTTLRRDVETNGRHAQVAFGTDWQTDAERRDLTINALYANEKGEIIDLVDGLADVETRTVRFIGDAAMRI
SEDYLRILRFFRFFAHYGSGRPDADGLRASARAKDKLGTLSAERVWSETKKLISARDPSRALLWMRQSGVLSEILPETEK
WGIDAIHGLVATEQALGWAADPMLRLAAIVPPDRERLAALAARLRLSKAEAAYLTHWASAPAVDPEMKETALDRLLYRQG
VEAVKTRLKLALASARADLSAGDTAMHKVARLSTLLTRAEKFRKPGFPLSGADVMAAGVEAGPKVGEVLKSLEEKWIDVN
FSLDRAALTARLKDMLEN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=410, Percent_Identity=31.4634146341463, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87081691, Length=210, Percent_Identity=30, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=411, Percent_Identity=34.0632603406326, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI281360112, Length=416, Percent_Identity=31.7307692307692, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI21357337, Length=416, Percent_Identity=31.7307692307692, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45675; Mature: 45675

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLAGQDWFEKPALKRIFALLNADGGEVRIVGGAVRNALMDLPVVDVDMATTVTPNVVV
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCEEECCCCCCCCHHHHH
ERAKAAGIKSVPTGIEHGTVTLVIDGEGFEVTTLRRDVETNGRHAQVAFGTDWQTDAERR
HHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHCCCCCEEEEEECCCCCCCCCCC
DLTINALYANEKGEIIDLVDGLADVETRTVRFIGDAAMRISEDYLRILRFFRFFAHYGSG
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDADGLRASARAKDKLGTLSAERVWSETKKLISARDPSRALLWMRQSGVLSEILPETEK
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHEEEEHHCCCHHHHCCCHHH
WGIDAIHGLVATEQALGWAADPMLRLAAIVPPDRERLAALAARLRLSKAEAAYLTHWASA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAVDPEMKETALDRLLYRQGVEAVKTRLKLALASARADLSAGDTAMHKVARLSTLLTRAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KFRKPGFPLSGADVMAAGVEAGPKVGEVLKSLEEKWIDVNFSLDRAALTARLKDMLEN
HHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSLAGQDWFEKPALKRIFALLNADGGEVRIVGGAVRNALMDLPVVDVDMATTVTPNVVV
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCEEECCCCCCCCHHHHH
ERAKAAGIKSVPTGIEHGTVTLVIDGEGFEVTTLRRDVETNGRHAQVAFGTDWQTDAERR
HHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHCCCCCEEEEEECCCCCCCCCCC
DLTINALYANEKGEIIDLVDGLADVETRTVRFIGDAAMRISEDYLRILRFFRFFAHYGSG
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPDADGLRASARAKDKLGTLSAERVWSETKKLISARDPSRALLWMRQSGVLSEILPETEK
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHEEEEHHCCCHHHHCCCHHH
WGIDAIHGLVATEQALGWAADPMLRLAAIVPPDRERLAALAARLRLSKAEAAYLTHWASA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAVDPEMKETALDRLLYRQGVEAVKTRLKLALASARADLSAGDTAMHKVARLSTLLTRAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KFRKPGFPLSGADVMAAGVEAGPKVGEVLKSLEEKWIDVNFSLDRAALTARLKDMLEN
HHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA