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The map label for this gene is prsA

Identifier: 159185103

GI number: 159185103

Start: 2186332

End: 2187264

Strand: Direct

Name: prsA

Synonym: Atu2218

Alternate gene names: 159185103

Gene position: 2186332-2187264 (Clockwise)

Preceding gene: 159185102

Following gene: 159185107

Centisome position: 76.94

GC content: 59.81

Gene sequence:

>933_bases
ATGAAGGTTTTCGCAGGCAATTCGAACCGGCACCTCGCCGAAGCGATCTGCAAGTATCTGAACGTTCCTCTCGGAAATGC
CACTGTAAAGCGGTTTGCTGACCAGGAAATATTCGTAGAAATCAGCGAGAACGTGCGCGGCGAGGACGTTTTTATCGTCC
AGTCCACCTCCTTCCCGGCAAATGATCATCTGATGGAACTGCTCATCATGATCGATGCCATGCGCCGCTCTTCGGCAAAA
CGTATCACCGCGGTGCTTCCCTATTTCGGTTACGCCCGCCAGGACCGCAAAGCCGGTCCCCGCACCCCGATTTCCGCCAA
GCTCGTTGCCAACCTGATTACCGAAGCCGGCGCCGACCGCGTTCTAACGCTCGATCTTCATGCCGGCCAGATCCAGGGTT
TTTTCGACATCCCCACCGACAACCTCTTCGCAGCGCCCATTCTCGCCCGCGACGTGAAGGACCATTACGACACCAACAAT
GTCATGGTCGTTTCGCCTGATGTCGGCGGCGTGGTTCGCGCCCGCGCCCTGTCGAAGCGCCTCGACTGTCTGCTGGCAAT
CGTCGACAAACGCCGTGACCGTCCCGGTGAATCCGAGGTCATGAACGTCATCGGCGATGTCTCCGGCAAGGATTGCATCC
TGATCGACGATATCATCGATTCCGGCGGCACGCTCTGCAACGCCGCCGAAGCGCTGCTGAAAAAAGGCGCGACCAGCGTT
ACCGCCTATATCACCCACGGTGTGCTTTCCGGCGGCGCGGTTGCCCGCGTGGCCTCCTCGAAGCTGCGCGAACTCGTCAT
CACCGACAGCATCCAGCCGACCACCGGTGTGCAATCCGCGCACAATATCCGCGTGGTCAGCACCGCAGGCCTGCTTGGCG
AGGCGATCAACCGCACCGCGCAGGAACAGTCGGTCTCCGGCCTGTTCGACTAA

Upstream 100 bases:

>100_bases
AAGGCTTTTCGGCCGGATGTTCCTCCCCCTTGAACGACGCTTGGTTACGGTGGATTGAGCCCCGTGGCGCAAGGCGATGG
CATGGAAACAGGCGGCCGCA

Downstream 100 bases:

>100_bases
CGGCTCCAGAAACCTCAATCGTCACGCTGAGCCGGCCCAACCCCGTCCAGCTTAGATGGCAGACTGCATTTATATCGAGG
TCGATTGCAGGAGAGGCATA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKVFAGNSNRHLAEAICKYLNVPLGNATVKRFADQEIFVEISENVRGEDVFIVQSTSFPANDHLMELLIMIDAMRRSSAK
RITAVLPYFGYARQDRKAGPRTPISAKLVANLITEAGADRVLTLDLHAGQIQGFFDIPTDNLFAAPILARDVKDHYDTNN
VMVVSPDVGGVVRARALSKRLDCLLAIVDKRRDRPGESEVMNVIGDVSGKDCILIDDIIDSGGTLCNAAEALLKKGATSV
TAYITHGVLSGGAVARVASSKLRELVITDSIQPTTGVQSAHNIRVVSTAGLLGEAINRTAQEQSVSGLFD

Sequences:

>Translated_310_residues
MKVFAGNSNRHLAEAICKYLNVPLGNATVKRFADQEIFVEISENVRGEDVFIVQSTSFPANDHLMELLIMIDAMRRSSAK
RITAVLPYFGYARQDRKAGPRTPISAKLVANLITEAGADRVLTLDLHAGQIQGFFDIPTDNLFAAPILARDVKDHYDTNN
VMVVSPDVGGVVRARALSKRLDCLLAIVDKRRDRPGESEVMNVIGDVSGKDCILIDDIIDSGGTLCNAAEALLKKGATSV
TAYITHGVLSGGAVARVASSKLRELVITDSIQPTTGVQSAHNIRVVSTAGLLGEAINRTAQEQSVSGLFD
>Mature_310_residues
MKVFAGNSNRHLAEAICKYLNVPLGNATVKRFADQEIFVEISENVRGEDVFIVQSTSFPANDHLMELLIMIDAMRRSSAK
RITAVLPYFGYARQDRKAGPRTPISAKLVANLITEAGADRVLTLDLHAGQIQGFFDIPTDNLFAAPILARDVKDHYDTNN
VMVVSPDVGGVVRARALSKRLDCLLAIVDKRRDRPGESEVMNVIGDVSGKDCILIDDIIDSGGTLCNAAEALLKKGATSV
TAYITHGVLSGGAVARVASSKLRELVITDSIQPTTGVQSAHNIRVVSTAGLLGEAINRTAQEQSVSGLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=310, Percent_Identity=46.1290322580645, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI28557709, Length=310, Percent_Identity=45.4838709677419, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4506129, Length=308, Percent_Identity=45.1298701298701, Blast_Score=285, Evalue=3e-77,
Organism=Homo sapiens, GI84875539, Length=311, Percent_Identity=44.6945337620579, Blast_Score=280, Evalue=9e-76,
Organism=Homo sapiens, GI4506133, Length=342, Percent_Identity=34.7953216374269, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=32.7485380116959, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=34.0277777777778, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=34.0277777777778, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=34.0277777777778, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=34.0277777777778, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=54.1666666666667, Blast_Score=347, Evalue=6e-97,
Organism=Caenorhabditis elegans, GI25149168, Length=310, Percent_Identity=45.1612903225806, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=310, Percent_Identity=45.1612903225806, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=310, Percent_Identity=45.1612903225806, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=45.1298701298701, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=337, Percent_Identity=34.1246290801187, Blast_Score=191, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6321776, Length=310, Percent_Identity=44.1935483870968, Blast_Score=265, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6320946, Length=311, Percent_Identity=43.7299035369775, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6319403, Length=312, Percent_Identity=44.2307692307692, Blast_Score=257, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=42.3469387755102, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324511, Length=105, Percent_Identity=39.0476190476191, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355239, Length=310, Percent_Identity=45.8064516129032, Blast_Score=280, Evalue=8e-76,
Organism=Drosophila melanogaster, GI45551540, Length=333, Percent_Identity=43.2432432432432, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=31.3390313390313, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=31.3390313390313, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=31.3390313390313, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=31.3390313390313, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24651462, Length=370, Percent_Identity=30.8108108108108, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651464, Length=370, Percent_Identity=30.8108108108108, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552010, Length=370, Percent_Identity=30.8108108108108, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_AGRT5 (Q8UDA9)

Other databases:

- EMBL:   AE007869
- PIR:   AI2848
- PIR:   G97625
- RefSeq:   NP_355175.2
- ProteinModelPortal:   Q8UDA9
- SMR:   Q8UDA9
- STRING:   Q8UDA9
- GeneID:   1134256
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2218
- eggNOG:   COG0462
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- PhylomeDB:   Q8UDA9
- ProtClustDB:   PRK01259
- BioCyc:   ATUM176299-1:ATU2218-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 33344; Mature: 33344

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFAGNSNRHLAEAICKYLNVPLGNATVKRFADQEIFVEISENVRGEDVFIVQSTSFPA
CEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
NDHLMELLIMIDAMRRSSAKRITAVLPYFGYARQDRKAGPRTPISAKLVANLITEAGADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLFAAPILARDVKDHYDTNNVMVVSPDVGGVVRARALSKR
EEEEEECCCCCCEEEECCCCCEEECHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
LDCLLAIVDKRRDRPGESEVMNVIGDVSGKDCILIDDIIDSGGTLCNAAEALLKKGATSV
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCHH
TAYITHGVLSGGAVARVASSKLRELVITDSIQPTTGVQSAHNIRVVSTAGLLGEAINRTA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
QEQSVSGLFD
HHHHHCCCCC
>Mature Secondary Structure
MKVFAGNSNRHLAEAICKYLNVPLGNATVKRFADQEIFVEISENVRGEDVFIVQSTSFPA
CEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCC
NDHLMELLIMIDAMRRSSAKRITAVLPYFGYARQDRKAGPRTPISAKLVANLITEAGADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
VLTLDLHAGQIQGFFDIPTDNLFAAPILARDVKDHYDTNNVMVVSPDVGGVVRARALSKR
EEEEEECCCCCCEEEECCCCCEEECHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
LDCLLAIVDKRRDRPGESEVMNVIGDVSGKDCILIDDIIDSGGTLCNAAEALLKKGATSV
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCHH
TAYITHGVLSGGAVARVASSKLRELVITDSIQPTTGVQSAHNIRVVSTAGLLGEAINRTA
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
QEQSVSGLFD
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194