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The map label for this gene is carA

Identifier: 159185084

GI number: 159185084

Start: 2140140

End: 2141345

Strand: Direct

Name: carA

Synonym: Atu2170

Alternate gene names: 159185084

Gene position: 2140140-2141345 (Clockwise)

Preceding gene: 15889444

Following gene: 159185085

Centisome position: 75.32

GC content: 61.86

Gene sequence:

>1206_bases
ATGACCGAGACAGCGCCCTGGACTACCCGCAAACCCACCGCAATGCTTGTTCTAGCCGATGGCACGGTGATTGAAGGAAC
AGGCATCGGTGCAACCGGCAAGGTTCAGGCCGAAGTCTGCTTCAACACGGCGCTGACGGGTTACGAGGAAATCCTCACCG
ACCCCTCCTATCTCGGCCAGATCGTCACCTTCACCTTCCCTCATATCGGCAATGTCGGCACCAATGAGGAAGACATCGAA
GACCTGACGCCCGCCGCCCGTCGCGGCGCCGTCGGCGTCATCTTCAAGGCCGATATCACCGACCCCTCGAACTTCCGCGC
CGTCAAGCATCTGGATGCCTGGCTGAAGGCGCGTGGGGTCATCGGCCTCTGCGGTATCGATACACGCGCGCTGACGGCCT
GGATCCGCGAAAACGGTGCGCCGAACGCGGTCATCGCTCATGACCCGAACGGCGTCTTTGACATCGAAGCGCTGAAGGCC
GAAGCCAAGGCATGGAGCGGTCTCGTCGGCCTCGACCTCGCCATCGAAGCGACGTCCGGCCAGTCCTCCACCTGGACGGA
AACGCCGTGGGTATGGAACAAGGGTTACGGCACGCTCGGTGAAGCCGATGCGAAATACCACGTCGTCTGCGTGGATTTCG
GCGTCAAGCGCAACATCCTGCGCCTGTTTGCCGGCCTCGATTGCAAGGTGACGGTTGTTCCGGCGCAGACCTCGGCTGAA
GATATTCTGGCGCTGAAGCCGGATGGCGTCTTTCTCTCCAACGGTCCGGGAGATCCGGCCGCGACGGGCGAATATGCCGT
GCCTGTTATCCAGAACCTCATCAAGAGCGAACTGCCGATCTTCGGCATCTGCCTCGGTCACCAGATGCTTGGCCTCGCCG
TTGGCGCGAAGACCGAAAAGATGCATCAGGGCCATCACGGCGCCAACCATCCGGTCAAGGACTTCACCACCGGCAAGGTG
GAAATCGTCTCTATGAACCACGGCTTTGCGGTCGACACCAAATCGCTGCCCGAGGGCGTCGAGGAAACCCACACGTCGCT
GTTCGACGGCACCAATTGCGGCCTGCGCATCGTCGGCAAGCCGGTCTTCTCCGTCCAGCACCATCCGGAAGCATCGCCCG
GCCCGCAGGACAGCCACTATCTCTTCCGCCGCTTCGTCAACCTGCTGCGCGAAAACAAGGGTGAGGCAGCACTCGCCGAG
CGCTGA

Upstream 100 bases:

>100_bases
TTATCGCGCGCCCCTTTATGTCACGAAGCCTGCGACAGAAATGGCCCCTTACCCCCTGCCTTTCAAGAGGCGGGGTGCGA
AGCGGCAGAAACGGAACGAG

Downstream 100 bases:

>100_bases
GACAATCCGAGGAAATCGTGAAAGAACCGCCGCCGCTCCCGAGAGCTGCGGTCGGACAACTGATTGTAATACTCGGCGGA
AGGCAGGGTCTGCGAGACCC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTETAPWTTRKPTAMLVLADGTVIEGTGIGATGKVQAEVCFNTALTGYEEILTDPSYLGQIVTFTFPHIGNVGTNEEDIE
DLTPAARRGAVGVIFKADITDPSNFRAVKHLDAWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKA
EAKAWSGLVGLDLAIEATSGQSSTWTETPWVWNKGYGTLGEADAKYHVVCVDFGVKRNILRLFAGLDCKVTVVPAQTSAE
DILALKPDGVFLSNGPGDPAATGEYAVPVIQNLIKSELPIFGICLGHQMLGLAVGAKTEKMHQGHHGANHPVKDFTTGKV
EIVSMNHGFAVDTKSLPEGVEETHTSLFDGTNCGLRIVGKPVFSVQHHPEASPGPQDSHYLFRRFVNLLRENKGEAALAE
R

Sequences:

>Translated_401_residues
MTETAPWTTRKPTAMLVLADGTVIEGTGIGATGKVQAEVCFNTALTGYEEILTDPSYLGQIVTFTFPHIGNVGTNEEDIE
DLTPAARRGAVGVIFKADITDPSNFRAVKHLDAWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKA
EAKAWSGLVGLDLAIEATSGQSSTWTETPWVWNKGYGTLGEADAKYHVVCVDFGVKRNILRLFAGLDCKVTVVPAQTSAE
DILALKPDGVFLSNGPGDPAATGEYAVPVIQNLIKSELPIFGICLGHQMLGLAVGAKTEKMHQGHHGANHPVKDFTTGKV
EIVSMNHGFAVDTKSLPEGVEETHTSLFDGTNCGLRIVGKPVFSVQHHPEASPGPQDSHYLFRRFVNLLRENKGEAALAE
R
>Mature_400_residues
TETAPWTTRKPTAMLVLADGTVIEGTGIGATGKVQAEVCFNTALTGYEEILTDPSYLGQIVTFTFPHIGNVGTNEEDIED
LTPAARRGAVGVIFKADITDPSNFRAVKHLDAWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKAE
AKAWSGLVGLDLAIEATSGQSSTWTETPWVWNKGYGTLGEADAKYHVVCVDFGVKRNILRLFAGLDCKVTVVPAQTSAED
ILALKPDGVFLSNGPGDPAATGEYAVPVIQNLIKSELPIFGICLGHQMLGLAVGAKTEKMHQGHHGANHPVKDFTTGKVE
IVSMNHGFAVDTKSLPEGVEETHTSLFDGTNCGLRIVGKPVFSVQHHPEASPGPQDSHYLFRRFVNLLRENKGEAALAER

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=398, Percent_Identity=34.9246231155779, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI169790915, Length=398, Percent_Identity=34.6733668341709, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI21361331, Length=398, Percent_Identity=34.6733668341709, Blast_Score=196, Evalue=4e-50,
Organism=Escherichia coli, GI1786215, Length=387, Percent_Identity=49.3540051679587, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI193204318, Length=395, Percent_Identity=37.9746835443038, Blast_Score=212, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6324878, Length=387, Percent_Identity=35.4005167958656, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=31.6708229426434, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45555749, Length=391, Percent_Identity=34.7826086956522, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24642586, Length=391, Percent_Identity=34.7826086956522, Blast_Score=191, Evalue=5e-49,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_AGRT5 (Q8UDF7)

Other databases:

- EMBL:   AE007869
- PIR:   AI2842
- PIR:   B97620
- RefSeq:   NP_355130.2
- ProteinModelPortal:   Q8UDF7
- SMR:   Q8UDF7
- STRING:   Q8UDF7
- GeneID:   1134208
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2170
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   Q8UDF7
- ProtClustDB:   PRK12564
- BioCyc:   ATUM176299-1:ATU2170-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42940; Mature: 42809

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 284-284 ACT_SITE 368-368 ACT_SITE 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETAPWTTRKPTAMLVLADGTVIEGTGIGATGKVQAEVCFNTALTGYEEILTDPSYLGQ
CCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHCE
IVTFTFPHIGNVGTNEEDIEDLTPAARRGAVGVIFKADITDPSNFRAVKHLDAWLKARGV
EEEEECCCCCCCCCCHHHHHHCCHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
IGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKAEAKAWSGLVGLDLAIEATSG
EEEECCCHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCEEEEEEEEEECCC
QSSTWTETPWVWNKGYGTLGEADAKYHVVCVDFGVKRNILRLFAGLDCKVTVVPAQTSAE
CCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCC
DILALKPDGVFLSNGPGDPAATGEYAVPVIQNLIKSELPIFGICLGHQMLGLAVGAKTEK
CEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHH
MHQGHHGANHPVKDFTTGKVEIVSMNHGFAVDTKSLPEGVEETHTSLFDGTNCGLRIVGK
HHCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECC
PVFSVQHHPEASPGPQDSHYLFRRFVNLLRENKGEAALAER
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TETAPWTTRKPTAMLVLADGTVIEGTGIGATGKVQAEVCFNTALTGYEEILTDPSYLGQ
CCCCCCCCCCCEEEEEEECCEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHCCHHHHCE
IVTFTFPHIGNVGTNEEDIEDLTPAARRGAVGVIFKADITDPSNFRAVKHLDAWLKARGV
EEEEECCCCCCCCCCHHHHHHCCHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCC
IGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKAEAKAWSGLVGLDLAIEATSG
EEEECCCHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCEEEEEEEEEECCC
QSSTWTETPWVWNKGYGTLGEADAKYHVVCVDFGVKRNILRLFAGLDCKVTVVPAQTSAE
CCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCC
DILALKPDGVFLSNGPGDPAATGEYAVPVIQNLIKSELPIFGICLGHQMLGLAVGAKTEK
CEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHH
MHQGHHGANHPVKDFTTGKVEIVSMNHGFAVDTKSLPEGVEETHTSLFDGTNCGLRIVGK
HHCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEECC
PVFSVQHHPEASPGPQDSHYLFRRFVNLLRENKGEAALAER
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194