Click here to switch to the map view.

The map label for this gene is iivG [H]

Identifier: 159185062

GI number: 159185062

Start: 2093537

End: 2095189

Strand: Direct

Name: iivG [H]

Synonym: Atu2128

Alternate gene names: 159185062

Gene position: 2093537-2095189 (Clockwise)

Preceding gene: 159185061

Following gene: 15889410

Centisome position: 73.68

GC content: 62.92

Gene sequence:

>1653_bases
ATGAAAAGAACTGGTGGCCAACTGATCGTCGAAGCGCTGAAGGCCAATGGCGTCTCGCGCGTTTCCTGCGTTCCGGGCGA
AAGCTACCTGGCGGTGCTGGATGCACTTTATGAAAGCGGCATCGAAACCGTCGTCTGCCGCCAGGAAGGCGGCGCCGCCA
TGATGGCTGACACCTGGGGCCGCCTGACGGGCGAACCAGGCATCTGCATGGTGACGCGCGGACCGGGCGCCACCAACGCA
TCGGCCGGCCTGCACATTGCGAGACAGGATTCCATTCCGATGATCCTCTTCATCGGCCAGGTACAGCGCGAAGCCCGCGA
GCGCGAGGCGTTTCAGGAAGTCGAATACCGCCGCGCCTTCACCGAATTCGCCAAATGGGTGGGCGAGATCGACGATGCCC
GCCGCATCCCCGAATTCGTCACCCGTGCCTTCGCCGTTGCCACCTCCGGCCGCCCCGGCCCGGTGGTTCTGACGCTGCCC
GAAGACATGCTGGTGGAGGAGGTCGAAGCGCCCGAGGCAAAGCCCTATATGCCGGTGGAGGCGCATCCCGGCCCTTCGCA
GATGACGGCCTTCGCAAAAATGCTGGGCGAGGCGAAACGGCCGATTTTCATTATCGGCGGTACACGCTGGTCCGAAGAAA
GCGTGGCAACCTTCCGGGCATTTGCCGAAAACCACAAGCTGCCCGTCGGCTGTTCCTTCCGCCGCCAGATGCTGTTCGAT
CATCTGAGCCCCTCTTATGCCGGAGATGTCGGCATCGGCATCAACCCGGCCTTAGCGAAGGAGATCAAGGAATCCGATCT
GGTGGTGCTGCTCGGCGGTCGCCTCTCGGAAATGCCGTCCTCCGGTTATACGCTCATCGACATCCCCTACCCGTCGCAGA
AGCTGATCCATATTTATCCCGAAGCCGAAGAGCTCGGCCGCGTCTATCGCCCCGATCTGGCGATCTGTGCTGCGCCGGAT
GATTTCGTCGCCGTCCTCTCCTCGCTCACCCTTTCCGGCAGCGCCGCCTGGGCGGAACGCACCGCCGCCATGCACGCGGC
TTACCTCGCCTGGTCGACGCCGCCGCAGACCGGCCCCGGCACGGTGCAGATGGGGCCGATCATGGACTGGATCGAGGCCA
ATGTGGCCGACGACGCCATCTTCACCAATGGCGCCGGCAATTATGCCACCTGGCTGCACCGCTTCCACCGCTTCCGCCGC
TACAACACGCAATCGGCCCCGACCTCCGGTTCCATGGGTTACGGCCTGCCGGCGGCTGTCGCCGCCAAGCATCTGTTCCC
GGAGCGTGAGGTGATCTGCTTTGCCGGTGACGGCTGTTTCATGATGCACGGACAGGAATTCATCACTGCCGTGCGTTATG
GCCTGCCGATCATCACCGTGCTTGTTAACAATGGCACCTATGGCACCATTCGCATGCATCAGGAGCGGGAATACCCCGGC
CGTGTCAGCGGCACCGATCTCGTCAATCCGGATTTCAACGCTTTTGCCAAGGCCTATGGCGGCCATGGCGAAACGGTGGA
AAGAACGCAAGACTTCGCCGCCGCCTTCGAGCGGGCGCGTGCAAGCGGCAAGCCTGGCATCATCGAGGTGAAGCTGGACC
CGGAAGCGATCACGCCGACGCGCACGCTGACGCAGATCAGGACAAAAAGCTGA

Upstream 100 bases:

>100_bases
TGCGTTATGAGCGCCCGAGCCCGAAGCTCGGCGAACATCAGGCGCAGGTGCTGGCCGAACTGGAAACTATCGAAAGGACC
GCGACGCCATGAAGGCAGGA

Downstream 100 bases:

>100_bases
CATGACGCATGGACATGCCTCCGGCATGGTGTAAATCCTGACGCTCGCTGCACGTCATCCTCGGGCTTGTCCCGAGGATC
CAACCACGTCGCGTTAAATC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKRTGGQLIVEALKANGVSRVSCVPGESYLAVLDALYESGIETVVCRQEGGAAMMADTWGRLTGEPGICMVTRGPGATNA
SAGLHIARQDSIPMILFIGQVQREAREREAFQEVEYRRAFTEFAKWVGEIDDARRIPEFVTRAFAVATSGRPGPVVLTLP
EDMLVEEVEAPEAKPYMPVEAHPGPSQMTAFAKMLGEAKRPIFIIGGTRWSEESVATFRAFAENHKLPVGCSFRRQMLFD
HLSPSYAGDVGIGINPALAKEIKESDLVVLLGGRLSEMPSSGYTLIDIPYPSQKLIHIYPEAEELGRVYRPDLAICAAPD
DFVAVLSSLTLSGSAAWAERTAAMHAAYLAWSTPPQTGPGTVQMGPIMDWIEANVADDAIFTNGAGNYATWLHRFHRFRR
YNTQSAPTSGSMGYGLPAAVAAKHLFPEREVICFAGDGCFMMHGQEFITAVRYGLPIITVLVNNGTYGTIRMHQEREYPG
RVSGTDLVNPDFNAFAKAYGGHGETVERTQDFAAAFERARASGKPGIIEVKLDPEAITPTRTLTQIRTKS

Sequences:

>Translated_550_residues
MKRTGGQLIVEALKANGVSRVSCVPGESYLAVLDALYESGIETVVCRQEGGAAMMADTWGRLTGEPGICMVTRGPGATNA
SAGLHIARQDSIPMILFIGQVQREAREREAFQEVEYRRAFTEFAKWVGEIDDARRIPEFVTRAFAVATSGRPGPVVLTLP
EDMLVEEVEAPEAKPYMPVEAHPGPSQMTAFAKMLGEAKRPIFIIGGTRWSEESVATFRAFAENHKLPVGCSFRRQMLFD
HLSPSYAGDVGIGINPALAKEIKESDLVVLLGGRLSEMPSSGYTLIDIPYPSQKLIHIYPEAEELGRVYRPDLAICAAPD
DFVAVLSSLTLSGSAAWAERTAAMHAAYLAWSTPPQTGPGTVQMGPIMDWIEANVADDAIFTNGAGNYATWLHRFHRFRR
YNTQSAPTSGSMGYGLPAAVAAKHLFPEREVICFAGDGCFMMHGQEFITAVRYGLPIITVLVNNGTYGTIRMHQEREYPG
RVSGTDLVNPDFNAFAKAYGGHGETVERTQDFAAAFERARASGKPGIIEVKLDPEAITPTRTLTQIRTKS
>Mature_550_residues
MKRTGGQLIVEALKANGVSRVSCVPGESYLAVLDALYESGIETVVCRQEGGAAMMADTWGRLTGEPGICMVTRGPGATNA
SAGLHIARQDSIPMILFIGQVQREAREREAFQEVEYRRAFTEFAKWVGEIDDARRIPEFVTRAFAVATSGRPGPVVLTLP
EDMLVEEVEAPEAKPYMPVEAHPGPSQMTAFAKMLGEAKRPIFIIGGTRWSEESVATFRAFAENHKLPVGCSFRRQMLFD
HLSPSYAGDVGIGINPALAKEIKESDLVVLLGGRLSEMPSSGYTLIDIPYPSQKLIHIYPEAEELGRVYRPDLAICAAPD
DFVAVLSSLTLSGSAAWAERTAAMHAAYLAWSTPPQTGPGTVQMGPIMDWIEANVADDAIFTNGAGNYATWLHRFHRFRR
YNTQSAPTSGSMGYGLPAAVAAKHLFPEREVICFAGDGCFMMHGQEFITAVRYGLPIITVLVNNGTYGTIRMHQEREYPG
RVSGTDLVNPDFNAFAKAYGGHGETVERTQDFAAAFERARASGKPGIIEVKLDPEAITPTRTLTQIRTKS

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=25.4448398576512, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI21361361, Length=556, Percent_Identity=24.1007194244604, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=30.1094890510949, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=28.6225402504472, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=26.7631103074141, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1788716, Length=565, Percent_Identity=25.4867256637168, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=25.7042253521127, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=548, Percent_Identity=26.6423357664234, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=548, Percent_Identity=26.6423357664234, Blast_Score=132, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=567, Percent_Identity=23.4567901234568, Blast_Score=112, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=26.9162210338681, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323073, Length=546, Percent_Identity=23.4432234432234, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320816, Length=286, Percent_Identity=21.3286713286713, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=26.0945709281961, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59927; Mature: 59927

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTGGQLIVEALKANGVSRVSCVPGESYLAVLDALYESGIETVVCRQEGGAAMMADTWG
CCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCC
RLTGEPGICMVTRGPGATNASAGLHIARQDSIPMILFIGQVQREAREREAFQEVEYRRAF
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TEFAKWVGEIDDARRIPEFVTRAFAVATSGRPGPVVLTLPEDMLVEEVEAPEAKPYMPVE
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEE
AHPGPSQMTAFAKMLGEAKRPIFIIGGTRWSEESVATFRAFAENHKLPVGCSFRRQMLFD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
HLSPSYAGDVGIGINPALAKEIKESDLVVLLGGRLSEMPSSGYTLIDIPYPSQKLIHIYP
HCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHCCCCCCCEEEECCCCCCEEEEEEC
EAEELGRVYRPDLAICAAPDDFVAVLSSLTLSGSAAWAERTAAMHAAYLAWSTPPQTGPG
CHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCC
TVQMGPIMDWIEANVADDAIFTNGAGNYATWLHRFHRFRRYNTQSAPTSGSMGYGLPAAV
CEEECCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AAKHLFPEREVICFAGDGCFMMHGQEFITAVRYGLPIITVLVNNGTYGTIRMHQEREYPG
HHHHCCCCCCEEEEECCCEEEECCHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCC
RVSGTDLVNPDFNAFAKAYGGHGETVERTQDFAAAFERARASGKPGIIEVKLDPEAITPT
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
RTLTQIRTKS
HHHHHHHCCC
>Mature Secondary Structure
MKRTGGQLIVEALKANGVSRVSCVPGESYLAVLDALYESGIETVVCRQEGGAAMMADTWG
CCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCC
RLTGEPGICMVTRGPGATNASAGLHIARQDSIPMILFIGQVQREAREREAFQEVEYRRAF
CCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
TEFAKWVGEIDDARRIPEFVTRAFAVATSGRPGPVVLTLPEDMLVEEVEAPEAKPYMPVE
HHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCEE
AHPGPSQMTAFAKMLGEAKRPIFIIGGTRWSEESVATFRAFAENHKLPVGCSFRRQMLFD
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
HLSPSYAGDVGIGINPALAKEIKESDLVVLLGGRLSEMPSSGYTLIDIPYPSQKLIHIYP
HCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHCCCCCCCEEEECCCCCCEEEEEEC
EAEELGRVYRPDLAICAAPDDFVAVLSSLTLSGSAAWAERTAAMHAAYLAWSTPPQTGPG
CHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCC
TVQMGPIMDWIEANVADDAIFTNGAGNYATWLHRFHRFRRYNTQSAPTSGSMGYGLPAAV
CEEECCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AAKHLFPEREVICFAGDGCFMMHGQEFITAVRYGLPIITVLVNNGTYGTIRMHQEREYPG
HHHHCCCCCCEEEEECCCEEEECCHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCC
RVSGTDLVNPDFNAFAKAYGGHGETVERTQDFAAAFERARASGKPGIIEVKLDPEAITPT
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
RTLTQIRTKS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]