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The map label for this gene is metF [H]

Identifier: 159185058

GI number: 159185058

Start: 2087408

End: 2088298

Strand: Reverse

Name: metF [H]

Synonym: Atu2123

Alternate gene names: 159185058

Gene position: 2088298-2087408 (Counterclockwise)

Preceding gene: 15889405

Following gene: 159185053

Centisome position: 73.49

GC content: 60.94

Gene sequence:

>891_bases
TTGAGGTCTGAAACCGCACGCCGTGATGGCGATATCCGGTTGTCTTTCGAATTTTTCCCGCCCAAGAATGCCGAGATGGA
CGCCCAGCTCTGGGAAACGGTTGCCGAGCTGAAGAAGTGGGATCCGGATTTTGTGTCCGTCACCTATGGCGCTGGTGGTT
CCACCAAGGCGCCGACGCTGGATGCCGTGCGCCGGATGATCGGTGACAGCCATCTGCCGACCGCTGCGCATCTGACCTGC
GTCGACGCCAGCCGTGATGATGTGCATCAGGTCATCGAAGAGTTTCGCAACGCCGGCGTTCGCCATTTCGTGGCGCTGCG
TGGCGATCCCTCCACTGGCATTGGCACGGCTTACCAGCCGCATCCGGAAGGTTACGAGAACGCCGCAGCGCTGGTGAAGG
GGCTGCGCGAGATCGGCGATTTCGAGATTTCCGTTTCCGCCTATCCGGAAAAACATCCTGAAAGCGCAAGCGAGACGGTC
GATATCGACATGCTGAAGCGCAAGGCCGACAATGGTGCGACCCGGGCGCTGACCCAGTTCTTCTTCGATAATGACGTCTT
TGAACGCTACATGGAGCGTGTGACTGCGGCAGGCATCACCATTCCGGTGGTTCCGGGCATCATGCCGATCCAGAACCTGA
CCCAGCTCAAGCGTTTCGCCGGCCGCTGCGGCACCAGCGTTCCCAGATTTCTGGATGAGCGTTTCGAAGGCTATGACGAG
GATCTGGAAGGGCGTGCCCGCGTGGCCGCTGAAGTTGCGGCCGAGCAGATCGCCGATCTGCAACGGCGTGGCTTTAACGA
GTTCCACCTCTACACCATGAACCGCGCTCCGCTGGTCGACGCGGTGCTGGAAAATCTCGGGCTGCAGCGCCTGCGCCGCA
GCGCCGCCTGA

Upstream 100 bases:

>100_bases
CTGGACGTGACGATATGGGTTGCCCGCGACCAGCGGCTGCTGATGGCAGACGATGTGGTGCAAGAGGCGGTTTTACTTCG
CTCCGGGAGGGCATGATATG

Downstream 100 bases:

>100_bases
CGCCGGAAAGCCGATTACCGACATGAAAAAAGCGCAGGTCCCTCGGGGCCTGCGCTTTTTAAATTCGTGTCATCTACGGT
TCTTAACTGCGTTTGGTCTT

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRSETARRDGDIRLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTLDAVRRMIGDSHLPTAAHLTC
VDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQPHPEGYENAAALVKGLREIGDFEISVSAYPEKHPESASETV
DIDMLKRKADNGATRALTQFFFDNDVFERYMERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDE
DLEGRARVAAEVAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQRLRRSAA

Sequences:

>Translated_296_residues
MRSETARRDGDIRLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTLDAVRRMIGDSHLPTAAHLTC
VDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQPHPEGYENAAALVKGLREIGDFEISVSAYPEKHPESASETV
DIDMLKRKADNGATRALTQFFFDNDVFERYMERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDE
DLEGRARVAAEVAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQRLRRSAA
>Mature_296_residues
MRSETARRDGDIRLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTLDAVRRMIGDSHLPTAAHLTC
VDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQPHPEGYENAAALVKGLREIGDFEISVSAYPEKHPESASETV
DIDMLKRKADNGATRALTQFFFDNDVFERYMERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDE
DLEGRARVAAEVAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQRLRRSAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=297, Percent_Identity=31.986531986532, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1790377, Length=289, Percent_Identity=41.522491349481, Blast_Score=245, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI25147436, Length=290, Percent_Identity=30.6896551724138, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=290, Percent_Identity=30.6896551724138, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=288, Percent_Identity=30.2083333333333, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=30.9352517985612, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33005; Mature: 33005

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSETARRDGDIRLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTL
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DAVRRMIGDSHLPTAAHLTCVDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQP
HHHHHHHCCCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
HPEGYENAAALVKGLREIGDFEISVSAYPEKHPESASETVDIDMLKRKADNGATRALTQF
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
FFDNDVFERYMERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDE
HHCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
DLEGRARVAAEVAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQRLRRSAA
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSETARRDGDIRLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTL
CCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DAVRRMIGDSHLPTAAHLTCVDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQP
HHHHHHHCCCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
HPEGYENAAALVKGLREIGDFEISVSAYPEKHPESASETVDIDMLKRKADNGATRALTQF
CCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
FFDNDVFERYMERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDE
HHCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
DLEGRARVAAEVAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQRLRRSAA
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]