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The map label for this gene is pfp [H]

Identifier: 159185054

GI number: 159185054

Start: 2079158

End: 2080369

Strand: Direct

Name: pfp [H]

Synonym: Atu2115

Alternate gene names: 159185054

Gene position: 2079158-2080369 (Clockwise)

Preceding gene: 15889388

Following gene: 159185055

Centisome position: 73.17

GC content: 61.14

Gene sequence:

>1212_bases
ATGGCGAAACAGAAAGTCGCAATGCTGACCGCGGGTGGCCTCGCGCCCTGTCTCTCTTCGGCCGTGGGTGGCCTGATCGA
GCGCTATAGCGACATCGCGCCTGAAATCGACATCATCGCTTACCGCTCCGGTTATCAGGGCGTGCTTCTGGGCGAGCGTA
TCGAGATCACCAAGGATATGCGCGAAAAGGCGCATCTACTGCATCGTTACGGCGGCTCGCCGATCGGCAACAGCCGCGTC
AAGCTGACCAATGCCGCCGATTGCGCCAAGCGCGGCCTCGTCAAGGAAGGCGACAATCCGTTGCGCGTCGCCGCTGAACG
GCTGGCGGCTGACGGCATCACCATTCTCCATACTATCGGTGGCGACGACACCAACACCACCGCCGCGGACCTTGCCGCCT
ATCTCGGCGCCAACGGATATGACCTCACCGTCGTCGGCCTGCCGAAGACGGTGGATAATGACGTCGTGCCGATCAAGCAG
TCGCTCGGCGCCTGGACGGCCGCGGAAGTCGGTGCTGCCTTCTTCGACAATGTCAGCAACGAGCAGAGCGCGGCACCGAA
AACCTTCGTCATCCACGAAGTCATGGGCCGCCATTGCGGCTGGCTGACCGCCGCCACCGCCCGCGCCTATATCCAGAAAA
CCCGCGGCAACGACTATGTCGAAGGCCTGATGATGAACACGCAGATGAAGAACATCGACGGCATCTACCTGCCGGAAATG
GCCTTCGACATCGAGGCGGAAGCCGAGCGCCTCAAAGAGGTCATGGACAAACATGGCTATGTCACGCTGTTCGTTTCCGA
AGGTGCGGGCCTCGACGCCATCGTCGCCGAGCGGGAAGCTGCTGGCGAAGCCGTCAAGCGCGATGCCTTCGGCCATGTGA
AGATCGACACCATCAATGTTGGCGGCTGGTTCCAGAAGCAGTTCGCCGGCCTCATCGGCGCCGAGCGCTCCATGGTGCAG
AAGTCGGGCTATTTCGCCCGCTCCGCGCCCGCCAATGGCGACGATCTTCGCCTCATCCAGGGCATGGTCGATCTGGCGGT
CGAAAGCGCGCTCAATAAGGTCTCCGGGGTCACCGGTCATGACGAAGACCAGAATGGCAAATTGCGGACCATCGAGTTTC
CGCGTATCAAGGGCGGAAAGCATTTCGACCTCTCTGCAAAATGGTTTACCGAAGTGATGGAACACGTCGGTCAGCCGTTC
ACAGCGGCCTGA

Upstream 100 bases:

>100_bases
AATCAGCATTTTTCGTCATAAATTCACTTTACACCTACACGCGGCTGTCGCATCCATTTGCCAGACATGTCTTTGGGTAT
ACGCAAAAAGGAGAGCCATG

Downstream 100 bases:

>100_bases
TTTTACGATAGGCTAAAATAACGCGGTGACAGGGAGGGCAAGGAGGAAGAGAATGTCTGGACAGGCGTGGCTGATTTTCT
GTGCAGCATGTGCCGTTGTG

Product: pyrophosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPEIDIIAYRSGYQGVLLGERIEITKDMREKAHLLHRYGGSPIGNSRV
KLTNAADCAKRGLVKEGDNPLRVAAERLAADGITILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIKQ
SLGAWTAAEVGAAFFDNVSNEQSAAPKTFVIHEVMGRHCGWLTAATARAYIQKTRGNDYVEGLMMNTQMKNIDGIYLPEM
AFDIEAEAERLKEVMDKHGYVTLFVSEGAGLDAIVAEREAAGEAVKRDAFGHVKIDTINVGGWFQKQFAGLIGAERSMVQ
KSGYFARSAPANGDDLRLIQGMVDLAVESALNKVSGVTGHDEDQNGKLRTIEFPRIKGGKHFDLSAKWFTEVMEHVGQPF
TAA

Sequences:

>Translated_403_residues
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPEIDIIAYRSGYQGVLLGERIEITKDMREKAHLLHRYGGSPIGNSRV
KLTNAADCAKRGLVKEGDNPLRVAAERLAADGITILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIKQ
SLGAWTAAEVGAAFFDNVSNEQSAAPKTFVIHEVMGRHCGWLTAATARAYIQKTRGNDYVEGLMMNTQMKNIDGIYLPEM
AFDIEAEAERLKEVMDKHGYVTLFVSEGAGLDAIVAEREAAGEAVKRDAFGHVKIDTINVGGWFQKQFAGLIGAERSMVQ
KSGYFARSAPANGDDLRLIQGMVDLAVESALNKVSGVTGHDEDQNGKLRTIEFPRIKGGKHFDLSAKWFTEVMEHVGQPF
TAA
>Mature_402_residues
AKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPEIDIIAYRSGYQGVLLGERIEITKDMREKAHLLHRYGGSPIGNSRVK
LTNAADCAKRGLVKEGDNPLRVAAERLAADGITILHTIGGDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIKQS
LGAWTAAEVGAAFFDNVSNEQSAAPKTFVIHEVMGRHCGWLTAATARAYIQKTRGNDYVEGLMMNTQMKNIDGIYLPEMA
FDIEAEAERLKEVMDKHGYVTLFVSEGAGLDAIVAEREAAGEAVKRDAFGHVKIDTINVGGWFQKQFAGLIGAERSMVQK
SGYFARSAPANGDDLRLIQGMVDLAVESALNKVSGVTGHDEDQNGKLRTIEFPRIKGGKHFDLSAKWFTEVMEHVGQPFT
AA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family. PFP subfamily [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=287, Percent_Identity=27.5261324041812, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI266453748, Length=287, Percent_Identity=27.5261324041812, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI4505749, Length=287, Percent_Identity=27.5261324041812, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI266453619, Length=287, Percent_Identity=27.5261324041812, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790350, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=290, Percent_Identity=30, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022953
- InterPro:   IPR000023
- InterPro:   IPR011405 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43359; Mature: 43228

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPEIDIIAYRSGYQGVLLGERIEITKDM
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCHHHHHHHH
REKAHLLHRYGGSPIGNSRVKLTNAADCAKRGLVKEGDNPLRVAAERLAADGITILHTIG
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEC
GDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIKQSLGAWTAAEVGAAFFDNVSN
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
EQSAAPKTFVIHEVMGRHCGWLTAATARAYIQKTRGNDYVEGLMMNTQMKNIDGIYLPEM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCCEECCCH
AFDIEAEAERLKEVMDKHGYVTLFVSEGAGLDAIVAEREAAGEAVKRDAFGHVKIDTINV
HCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
GGWFQKQFAGLIGAERSMVQKSGYFARSAPANGDDLRLIQGMVDLAVESALNKVSGVTGH
CCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DEDQNGKLRTIEFPRIKGGKHFDLSAKWFTEVMEHVGQPFTAA
CCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKQKVAMLTAGGLAPCLSSAVGGLIERYSDIAPEIDIIAYRSGYQGVLLGERIEITKDM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCHHHHHHHH
REKAHLLHRYGGSPIGNSRVKLTNAADCAKRGLVKEGDNPLRVAAERLAADGITILHTIG
HHHHHHHHHHCCCCCCCCEEEECCHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEC
GDDTNTTAADLAAYLGANGYDLTVVGLPKTVDNDVVPIKQSLGAWTAAEVGAAFFDNVSN
CCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
EQSAAPKTFVIHEVMGRHCGWLTAATARAYIQKTRGNDYVEGLMMNTQMKNIDGIYLPEM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCCEECCCH
AFDIEAEAERLKEVMDKHGYVTLFVSEGAGLDAIVAEREAAGEAVKRDAFGHVKIDTINV
HCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
GGWFQKQFAGLIGAERSMVQKSGYFARSAPANGDDLRLIQGMVDLAVESALNKVSGVTGH
CCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
DEDQNGKLRTIEFPRIKGGKHFDLSAKWFTEVMEHVGQPFTAA
CCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653240; 1317210 [H]