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The map label for this gene is slt [H]

Identifier: 159185052

GI number: 159185052

Start: 2076523

End: 2077062

Strand: Reverse

Name: slt [H]

Synonym: Atu2112

Alternate gene names: 159185052

Gene position: 2077062-2076523 (Counterclockwise)

Preceding gene: 15889395

Following gene: 159185051

Centisome position: 73.1

GC content: 59.26

Gene sequence:

>540_bases
ATGAACAGGTTTGTTGTTGCTGTCGTGGCGGGCGTTGTTGCGCTCGCTTCGCAGGCGGGGATTTCTTTTGCCAAAGATGA
GGACGTCAAGACCAAGACCGTGACGCTGGAGACCTTCGTGCGCAAGCCCGGTTATCCCATTCCCGAAAAAAGCCTGCCTG
CCTCTCTCAAGAACGGCTATTCCGATCTGATCGTCAAATATGCCAAGCGTTACGGCGTTCCCACTAACCTGGCGCATGCC
GTGGTTTCGGTGGAAAGCAAGTTCAACCCCAAGGCCCGTGGCAGCGCCGGCGAGGTCGGGCTAATGCAGATCAAGCCGGC
AACGGCGCGGATGATGGGTTTTCGCGGCGCGACCAAAGCGCTCTACGATCCCGAGACGAATATTCGCTGGGGCATGCAAT
ATCTGGCGACGGCGCATCAGCTTGGCGGCGGCGAAGTGTGCAGCACCATCCTGCGTTACAATGCCGGCCACGGTGCCACC
CGCATGAACCCGGTCTCCAAGCGTTATTGCGGCAAGGTGCAGGCATTGCTGGCGAGCTGA

Upstream 100 bases:

>100_bases
AGGAGACCGGACAGCGGAGCGTCGTGATCGACTTTCCGGGCCCAAGCTGTTTGTGCCATTTCACTGTTTCTGTCATCCGA
TCTGGAGACTTTTTCTACAT

Downstream 100 bases:

>100_bases
GGCCGGCATCGATCATCTGCGAAATTGCGAAAAACGATTCCGGTTCGGGTCGGCATGCTGTAGTCAGAAACAAATCTGAT
ACGGAGCATGACATGCCGGA

Product: soluble lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNRFVVAVVAGVVALASQAGISFAKDEDVKTKTVTLETFVRKPGYPIPEKSLPASLKNGYSDLIVKYAKRYGVPTNLAHA
VVSVESKFNPKARGSAGEVGLMQIKPATARMMGFRGATKALYDPETNIRWGMQYLATAHQLGGGEVCSTILRYNAGHGAT
RMNPVSKRYCGKVQALLAS

Sequences:

>Translated_179_residues
MNRFVVAVVAGVVALASQAGISFAKDEDVKTKTVTLETFVRKPGYPIPEKSLPASLKNGYSDLIVKYAKRYGVPTNLAHA
VVSVESKFNPKARGSAGEVGLMQIKPATARMMGFRGATKALYDPETNIRWGMQYLATAHQLGGGEVCSTILRYNAGHGAT
RMNPVSKRYCGKVQALLAS
>Mature_179_residues
MNRFVVAVVAGVVALASQAGISFAKDEDVKTKTVTLETFVRKPGYPIPEKSLPASLKNGYSDLIVKYAKRYGVPTNLAHA
VVSVESKFNPKARGSAGEVGLMQIKPATARMMGFRGATKALYDPETNIRWGMQYLATAHQLGGGEVCSTILRYNAGHGAT
RMNPVSKRYCGKVQALLAS

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=109, Percent_Identity=37.6146788990826, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 19223; Mature: 19223

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVVAVVAGVVALASQAGISFAKDEDVKTKTVTLETFVRKPGYPIPEKSLPASLKNGY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCHHHCCH
SDLIVKYAKRYGVPTNLAHAVVSVESKFNPKARGSAGEVGLMQIKPATARMMGFRGATKA
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCH
LYDPETNIRWGMQYLATAHQLGGGEVCSTILRYNAGHGATRMNPVSKRYCGKVQALLAS
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRFVVAVVAGVVALASQAGISFAKDEDVKTKTVTLETFVRKPGYPIPEKSLPASLKNGY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCHHHCCH
SDLIVKYAKRYGVPTNLAHAVVSVESKFNPKARGSAGEVGLMQIKPATARMMGFRGATKA
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCH
LYDPETNIRWGMQYLATAHQLGGGEVCSTILRYNAGHGATRMNPVSKRYCGKVQALLAS
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]