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The map label for this gene is mraW

Identifier: 159185048

GI number: 159185048

Start: 2067546

End: 2068571

Strand: Reverse

Name: mraW

Synonym: Atu2102

Alternate gene names: 159185048

Gene position: 2068571-2067546 (Counterclockwise)

Preceding gene: 17935988

Following gene: 159185047

Centisome position: 72.8

GC content: 62.18

Gene sequence:

>1026_bases
ATGGCGGCGAATTCTGGCGGGCGATCTTCTGATGCCGATGGCGGACCTCAGCGTCACATCCCGGTCCTGCTTCGCGAAGT
CATTGCCGCGCTGGAACCAGCGCCCGGCAAGATCATTCTCGACGGAACCTTTGGCGCCGGCGGTTATACCCAGGCCATTC
TTGACCAGGGTGCCAACGTCATCGCGCTCGATCGCGATCCGACAGCAATCGCCGGCGGCGAGGCGATGGTTGTCGCCAAT
GGCGGCCGATTGAGCCTCATCCAGTCGCAATTTTCCGATCTTGCCAAGCATGCTCCCGAGGATGGGCTTGATGGCGTCGT
TCTCGATATCGGCGTTTCCTCCATGCAGATCGACGAGGCCGAGCGCGGTTTTTCCTTCCAGAAGAATGGTCCGCTCGACA
TGCGCATGTCCGCTTCGGGCGTTTCGGCGGCCGATGTCGTCAATCGTGCGAAGGTCGGCGATCTCATTCGCATCTTCGGT
TTTCTCGGTGAAGAAAAGCAGCCGGGCCGCATCGCCCGCGCCATCGAGAAGAAGCGGGCGGAAGAACCCTTCCGCACCAC
CCGCGATCTGGCTGGTCTCATCGAGATCGTCACGCCGCGCAAGGCGAAGGACAAGATCCATCCCGCAACACGGGTGTTCC
AGGCGCTGCGTGTTTTCGTCAATGATGAGCTTGGCGAGTTGGCGCAGGCGCTGTTCGCTGCAGAACGGTCCTTGAAGCCC
GGCGGCCGGCTGGTCGTCGTCACCTTCCATTCGCTGGAAGACCGTATCGTCAAGAAGTTCTTCTCCGACCGTTCGGGCAA
GGCCGCCGGTTCCCGGCATCTGCCAATGGTGGAGGACAAGCCCGCCATTTTCGAGAATATCGGCAAGCCGATGATCGCCG
CCAGCGACGAGGAGGCGGAGCTTAATCCGCGGGCCCGTTCCGCCAAGTTGCGCGCCGGCCTGCGCACCACCGCACCGGCG
CGGGCCGCGGATTTCTCGATCTTCGAGCTTCCCGATCTCGCCAGCCTTGAAAAGATGGGAGGCTGA

Upstream 100 bases:

>100_bases
TTCAGCTTTGGCAACCGAATGCGTTTCTGGCGGCGCAGGCGGAAGCAAGAGCGGGGCGCGGTTTTTCGGCTGCTCGCCCC
GCATAGGACGGGGACACGGA

Downstream 100 bases:

>100_bases
TATGCTGCGCACTTTCGATGTCATCTTGATGGGTGTGATGGTCGCTGCGGCCGTCGTGACCTATTCGATCAAGCACAAGG
CCGATCTGAAGCTTGAGGAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MAANSGGRSSDADGGPQRHIPVLLREVIAALEPAPGKIILDGTFGAGGYTQAILDQGANVIALDRDPTAIAGGEAMVVAN
GGRLSLIQSQFSDLAKHAPEDGLDGVVLDIGVSSMQIDEAERGFSFQKNGPLDMRMSASGVSAADVVNRAKVGDLIRIFG
FLGEEKQPGRIARAIEKKRAEEPFRTTRDLAGLIEIVTPRKAKDKIHPATRVFQALRVFVNDELGELAQALFAAERSLKP
GGRLVVVTFHSLEDRIVKKFFSDRSGKAAGSRHLPMVEDKPAIFENIGKPMIAASDEEAELNPRARSAKLRAGLRTTAPA
RAADFSIFELPDLASLEKMGG

Sequences:

>Translated_341_residues
MAANSGGRSSDADGGPQRHIPVLLREVIAALEPAPGKIILDGTFGAGGYTQAILDQGANVIALDRDPTAIAGGEAMVVAN
GGRLSLIQSQFSDLAKHAPEDGLDGVVLDIGVSSMQIDEAERGFSFQKNGPLDMRMSASGVSAADVVNRAKVGDLIRIFG
FLGEEKQPGRIARAIEKKRAEEPFRTTRDLAGLIEIVTPRKAKDKIHPATRVFQALRVFVNDELGELAQALFAAERSLKP
GGRLVVVTFHSLEDRIVKKFFSDRSGKAAGSRHLPMVEDKPAIFENIGKPMIAASDEEAELNPRARSAKLRAGLRTTAPA
RAADFSIFELPDLASLEKMGG
>Mature_340_residues
AANSGGRSSDADGGPQRHIPVLLREVIAALEPAPGKIILDGTFGAGGYTQAILDQGANVIALDRDPTAIAGGEAMVVANG
GRLSLIQSQFSDLAKHAPEDGLDGVVLDIGVSSMQIDEAERGFSFQKNGPLDMRMSASGVSAADVVNRAKVGDLIRIFGF
LGEEKQPGRIARAIEKKRAEEPFRTTRDLAGLIEIVTPRKAKDKIHPATRVFQALRVFVNDELGELAQALFAAERSLKPG
GRLVVVTFHSLEDRIVKKFFSDRSGKAAGSRHLPMVEDKPAIFENIGKPMIAASDEEAELNPRARSAKLRAGLRTTAPAR
AADFSIFELPDLASLEKMGG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=348, Percent_Identity=41.3793103448276, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI165377202, Length=205, Percent_Identity=41.9512195121951, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=43.4920634920635, Blast_Score=224, Evalue=6e-60,
Organism=Drosophila melanogaster, GI62472493, Length=342, Percent_Identity=41.2280701754386, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI28571637, Length=342, Percent_Identity=41.2280701754386, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_AGRT5 (P58745)

Other databases:

- EMBL:   AE007869
- PIR:   AG2834
- PIR:   C97612
- RefSeq:   NP_355067.2
- ProteinModelPortal:   P58745
- SMR:   P58745
- STRING:   P58745
- GeneID:   1134140
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2102
- NMPDR:   fig|176299.3.peg.470
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   P58745
- ProtClustDB:   PRK00050
- BioCyc:   ATUM176299-1:ATU2102-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36370; Mature: 36239

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: BINDING 64-64 BINDING 91-91 BINDING 109-109 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANSGGRSSDADGGPQRHIPVLLREVIAALEPAPGKIILDGTFGAGGYTQAILDQGANV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCE
IALDRDPTAIAGGEAMVVANGGRLSLIQSQFSDLAKHAPEDGLDGVVLDIGVSSMQIDEA
EEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHEHHHH
ERGFSFQKNGPLDMRMSASGVSAADVVNRAKVGDLIRIFGFLGEEKQPGRIARAIEKKRA
HCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
EEPFRTTRDLAGLIEIVTPRKAKDKIHPATRVFQALRVFVNDELGELAQALFAAERSLKP
CCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
GGRLVVVTFHSLEDRIVKKFFSDRSGKAAGSRHLPMVEDKPAIFENIGKPMIAASDEEAE
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCC
LNPRARSAKLRAGLRTTAPARAADFSIFELPDLASLEKMGG
CCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHCCC
>Mature Secondary Structure 
AANSGGRSSDADGGPQRHIPVLLREVIAALEPAPGKIILDGTFGAGGYTQAILDQGANV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCE
IALDRDPTAIAGGEAMVVANGGRLSLIQSQFSDLAKHAPEDGLDGVVLDIGVSSMQIDEA
EEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHEHHHH
ERGFSFQKNGPLDMRMSASGVSAADVVNRAKVGDLIRIFGFLGEEKQPGRIARAIEKKRA
HCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
EEPFRTTRDLAGLIEIVTPRKAKDKIHPATRVFQALRVFVNDELGELAQALFAAERSLKP
CCHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
GGRLVVVTFHSLEDRIVKKFFSDRSGKAAGSRHLPMVEDKPAIFENIGKPMIAASDEEAE
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCC
LNPRARSAKLRAGLRTTAPARAADFSIFELPDLASLEKMGG
CCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194