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The map label for this gene is glcD [H]

Identifier: 159185028

GI number: 159185028

Start: 2013171

End: 2014583

Strand: Reverse

Name: glcD [H]

Synonym: Atu2053

Alternate gene names: 159185028

Gene position: 2014583-2013171 (Counterclockwise)

Preceding gene: 15889340

Following gene: 15889338

Centisome position: 70.9

GC content: 60.86

Gene sequence:

>1413_bases
ATGGCACTGAAGGATGTGGTGGCGGGCAAGCGGAACGAAGAGGGTATCGCGTCTGCGCTCGCGGTGCTGAAGCAGCAGCT
GGGCGATAAGCTCCAGACGGGCCAGGCGTTTCGCGAGCAGCATGGCCATACCACCACCTACCTGACCCTGCAGGCGCCCG
ACGGTGTCGTTTTTGCCGAAAGCGCCGATGATGTGAAGGCCGTGGTAAAGGCCTGCGCGCAATACAAGGTGCCGGTCATT
CCCTTCGGAACGGGATCTTCGCTCGAAGGGCAGGTCAACGCGCCCAATGGCGGAATATGCATCGATTTCAGCCGGATGAA
TCGCATCATTGAGGTGAATGCCGAAGATCTTGACGTGACGGTGGAGCCCGGCGTGACGCGCGAGGATCTGAATGTCTATC
TGCGCGATACCGGCCTGTTCTTTCCGATCGATCCCGGCGCCAATGCTTCGATTGGCGGCATGGCCTCGACGCGCGCTTCC
GGCACCAATGCCGTGCGTTACGGCACGATGAAGGACAATGTTCTGGCCGTCACCGCCGTTGTCGCCAATGGTGAGGAAAT
CCGCACTGCGCGGCGCGCCCGCAAGTCGTCCGCCGGTTACGACCTGACGCGGCTTTTCGTGGGGGCCGAAGGCACGCTCG
GTGTCATCACCTCGGTGACGCTGCGGCTGCAGGGCATTCCGGAAAAGATTACCGGCGGTGTCTGCACCTTCGACAGTATC
AAGGCGGCCTGCGATGCCGTCATCATGACGATCCAGATGGGCGTTCCGGTCGCCCGCATCGAATTGCTTGACGAGGTGCA
GGTGCGCGCCTGCAATGCCTATTCCGGTCTGAACTATGCGGAAAAGCCGACACTCTTCGTGGAGTTCCATGGCACCGAGG
AGACGGCGGCGCTGCAATCGCAGCAGTTCGCCGAGATTGCAGCCGAATGCGGCAGCGTCGATTTCCGCTGGACGGGCGAT
GCGGCGGAGCGCAACAAGCTGTGGAAGGCGCGCCACGACGCCTATTGGGCGTCGCGGGCGCTGGCACCACATCTCGACGG
GCTTTCCACCGATGTCTGCGTGCCGATTTCACGGCTGGCCGATTGCGTCGTGGAAACGCAGCGGGATATTGCCGAGAACG
GCTTTCTGGCGCCGATCGTCGGCCATGCGGGTGATGGGAACTTCCATGTGCTTATTCTGTTTGATGGCAAGGACGCCGAA
AGCGTTGCGAAAACAGAGGCCTTTGTTGCCCGTCTCAACCGCCGCGCCATTGCCATGGACGGCACATGCACCGGCGAACA
TGGCATTGGCCAGGGCAAGATGAGTTTTCTTGCCGAAGAGGCAGGAAATGCACTGGATCTCATGCGCGCCATCAAAACGT
CGCTCGACCCCGATAACATCTTCAATCCGGGCAAATTGTTTCGGCTTGTCTGA

Upstream 100 bases:

>100_bases
CGCGATGCTGGACAAAGCGTTAACCTTATCCCATACCACCTGACGAAATTTCGTAACGGATCGAATCCGTTACGGGCAGA
TGTTGTGGTGGAGATTGACG

Downstream 100 bases:

>100_bases
TCGTTCTCATATTTGATTGGTACAAAAGGCTGCTTGCCGTTAAGGCAGAAAAGAATAGGTTTGCAGCGTTCGCGAAGGGA
GATGCAGGTCAGTGCTCGGG

Product: FAD dependent oxidoreductase

Products: (R)-acetoin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAESADDVKAVVKACAQYKVPVI
PFGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVTVEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRAS
GTNAVRYGTMKDNVLAVTAVVANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSI
KAACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQSQQFAEIAAECGSVDFRWTGD
AAERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLADCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAE
SVAKTEAFVARLNRRAIAMDGTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV

Sequences:

>Translated_470_residues
MALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAESADDVKAVVKACAQYKVPVI
PFGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVTVEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRAS
GTNAVRYGTMKDNVLAVTAVVANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSI
KAACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQSQQFAEIAAECGSVDFRWTGD
AAERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLADCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAE
SVAKTEAFVARLNRRAIAMDGTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV
>Mature_469_residues
ALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAESADDVKAVVKACAQYKVPVIP
FGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVTVEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRASG
TNAVRYGTMKDNVLAVTAVVANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSIK
AACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQSQQFAEIAAECGSVDFRWTGDA
AERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLADCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAES
VAKTEAFVARLNRRAIAMDGTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=444, Percent_Identity=50.9009009009009, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI37595754, Length=467, Percent_Identity=48.3940042826552, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI119964728, Length=420, Percent_Identity=25.7142857142857, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4501993, Length=472, Percent_Identity=25.4237288135593, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1789351, Length=418, Percent_Identity=29.1866028708134, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1787977, Length=506, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI48994907, Length=489, Percent_Identity=24.3353783231084, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994914, Length=142, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=471, Percent_Identity=43.7367303609342, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71992381, Length=471, Percent_Identity=42.4628450106157, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71992392, Length=376, Percent_Identity=48.6702127659575, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17534361, Length=442, Percent_Identity=27.1493212669683, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556096, Length=482, Percent_Identity=24.6887966804979, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320027, Length=469, Percent_Identity=38.8059701492537, Blast_Score=316, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6320023, Length=432, Percent_Identity=26.1574074074074, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6320764, Length=441, Percent_Identity=25.8503401360544, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI18921117, Length=427, Percent_Identity=29.03981264637, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639277, Length=427, Percent_Identity=29.03981264637, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24639275, Length=427, Percent_Identity=29.03981264637, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24653753, Length=459, Percent_Identity=26.3616557734205, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.4

Molecular weight: Translated: 50270; Mature: 50138

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAE
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHHCCCEEEEEEEECCCCEEEEC
SADDVKAVVKACAQYKVPVIPFGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVT
CCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCCEEEEHHHCCEEEEECCCCEEEE
VEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKDNVLAVTAV
ECCCCCHHHCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEE
VANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSI
EECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCHHHCCCEEEHHHH
KAACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQS
HHHHHHEEEEHCCCCCHHHHHHHHHHHHHEECHHCCCCCCCCCEEEEEECCCCHHHHHHH
QQFAEIAAECGSVDFRWTGDAAERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLA
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAESVAKTEAFVARLNRRAIAMD
HHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEC
GTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
ALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAE
CCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHHCCCEEEEEEEECCCCEEEEC
SADDVKAVVKACAQYKVPVIPFGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVT
CCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCCEEEEHHHCCEEEEECCCCEEEE
VEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKDNVLAVTAV
ECCCCCHHHCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEE
VANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSI
EECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEEEEECCCHHHCCCEEEHHHH
KAACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQS
HHHHHHEEEEHCCCCCHHHHHHHHHHHHHEECHHCCCCCCCCCEEEEEECCCCHHHHHHH
QQFAEIAAECGSVDFRWTGDAAERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLA
HHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
DCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAESVAKTEAFVARLNRRAIAMD
HHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEC
GTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R,R)-butane-2,3-diol; NAD+

Specific reaction: (R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]