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The map label for this gene is rbsB [H]

Identifier: 159184970

GI number: 159184970

Start: 1875825

End: 1876754

Strand: Reverse

Name: rbsB [H]

Synonym: Atu1899

Alternate gene names: 159184970

Gene position: 1876754-1875825 (Counterclockwise)

Preceding gene: 15889196

Following gene: 159184969

Centisome position: 66.05

GC content: 60.32

Gene sequence:

>930_bases
ATGAAATTTTCACGCATTCTGCTGACGTCCGCTGCCCTTCTTGCCCTGTCGCTCGGTTCGGCCAACGCCGCCGAAGTCAA
GAAGATCGGCCTTGCCGTCGCCAATCTGCAGGCGAACTTCTTCAACCAGATCAAGCAGGCCGTCGAAGCCGAAGCCAAGA
CGCGCGGCATTGCCGTCGTTACCGTCGATGCCAAGGGCGATGGCCCGACGCAGGTCAACCAGATCCAGGATCTGCTGACC
CAGAATATCGACGCGCTGATCTATATCCCAGCCGGTGCCGCTGCCGCCACCGTTCCGGTGAAGCTTGCGAAAGCTGCCGG
CGTTCCGGTCATCAACATCGACCGCAATGCCGATGGCGCGCCGGGTGATACCTTCCTTGCCACCGATTCCGTCGCTTCCG
CCAAGTCGGTTTGCGATTACATCATCAAACAGGCCGGTGGTGCCGGCAAGATGGTCATCATCCACGGCCAGAAGGGCACT
ACACCTGAAGTCGACCGCACGAAGGGCTGCATGGAATCGGTCAAGGCCAATTCCGGCGTGAAGATCGTTGCCGAGCAATG
GTCCAACATGTGGAGCCAGGATGAAGGCTTCCAGATCATGCAGAACATGCTGCAGGCCAATCCCGACGTCTCCATCGTCT
TTGCACAGGCAGACGGTCTGGCGCTCGGCGCGGCGCAGGCAATCAAGGTTGCCAATCCGTCGCAGAAGATCGTCGTTGGC
GGCTTCGATGGCGATACGGCAGCGCTTGAGGCACTGAAGAATGGCGTCTTCGACGTGACCGCGACGCAGCAGACCCAGAA
GATGGGCCGCATGGCTGTCGAGATGGCCGTCAAGATCGTCGGCGGCGAGAAGGTTCCGGCCGTCCAGCTCTCCGACGCGA
CGCTGACGACCAAGGAAAATGTCGCAGGCTTCATCGCCAACCATCCCTGA

Upstream 100 bases:

>100_bases
CAATAAACGACCCTCCCGATACGGGAGGCGTGAAAAACGACCGGAGGGGAGGAGGTTCGATCTGCCCGAAGGTCAAAAAT
CAACAAATGGAGGAATAATC

Downstream 100 bases:

>100_bases
TCCTTCTGCCCCGCGCGTCCAACCGGATGCGCGGGGCATTCATTGGTTTGTCTTTAGGCAATTTTGGACACAAAGCCGTG
GTGTATCGCCACTGCGATTA

Product: ABC transporter, substrate binding protein (ribose)

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKFSRILLTSAALLALSLGSANAAEVKKIGLAVANLQANFFNQIKQAVEAEAKTRGIAVVTVDAKGDGPTQVNQIQDLLT
QNIDALIYIPAGAAAATVPVKLAKAAGVPVINIDRNADGAPGDTFLATDSVASAKSVCDYIIKQAGGAGKMVIIHGQKGT
TPEVDRTKGCMESVKANSGVKIVAEQWSNMWSQDEGFQIMQNMLQANPDVSIVFAQADGLALGAAQAIKVANPSQKIVVG
GFDGDTAALEALKNGVFDVTATQQTQKMGRMAVEMAVKIVGGEKVPAVQLSDATLTTKENVAGFIANHP

Sequences:

>Translated_309_residues
MKFSRILLTSAALLALSLGSANAAEVKKIGLAVANLQANFFNQIKQAVEAEAKTRGIAVVTVDAKGDGPTQVNQIQDLLT
QNIDALIYIPAGAAAATVPVKLAKAAGVPVINIDRNADGAPGDTFLATDSVASAKSVCDYIIKQAGGAGKMVIIHGQKGT
TPEVDRTKGCMESVKANSGVKIVAEQWSNMWSQDEGFQIMQNMLQANPDVSIVFAQADGLALGAAQAIKVANPSQKIVVG
GFDGDTAALEALKNGVFDVTATQQTQKMGRMAVEMAVKIVGGEKVPAVQLSDATLTTKENVAGFIANHP
>Mature_309_residues
MKFSRILLTSAALLALSLGSANAAEVKKIGLAVANLQANFFNQIKQAVEAEAKTRGIAVVTVDAKGDGPTQVNQIQDLLT
QNIDALIYIPAGAAAATVPVKLAKAAGVPVINIDRNADGAPGDTFLATDSVASAKSVCDYIIKQAGGAGKMVIIHGQKGT
TPEVDRTKGCMESVKANSGVKIVAEQWSNMWSQDEGFQIMQNMLQANPDVSIVFAQADGLALGAAQAIKVANPSQKIVVG
GFDGDTAALEALKNGVFDVTATQQTQKMGRMAVEMAVKIVGGEKVPAVQLSDATLTTKENVAGFIANHP

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=292, Percent_Identity=34.2465753424658, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1790526, Length=305, Percent_Identity=27.5409836065574, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790674, Length=216, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789990, Length=290, Percent_Identity=28.6206896551724, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788898, Length=324, Percent_Identity=26.2345679012346, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788473, Length=299, Percent_Identity=24.4147157190635, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32014; Mature: 32014

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSRILLTSAALLALSLGSANAAEVKKIGLAVANLQANFFNQIKQAVEAEAKTRGIAVV
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
TVDAKGDGPTQVNQIQDLLTQNIDALIYIPAGAAAATVPVKLAKAAGVPVINIDRNADGA
EEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
PGDTFLATDSVASAKSVCDYIIKQAGGAGKMVIIHGQKGTTPEVDRTKGCMESVKANSGV
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
KIVAEQWSNMWSQDEGFQIMQNMLQANPDVSIVFAQADGLALGAAQAIKVANPSQKIVVG
EEEHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEECCHHEEEECCCCCEEEEE
GFDGDTAALEALKNGVFDVTATQQTQKMGRMAVEMAVKIVGGEKVPAVQLSDATLTTKEN
CCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECHHH
VAGFIANHP
CCEEECCCC
>Mature Secondary Structure
MKFSRILLTSAALLALSLGSANAAEVKKIGLAVANLQANFFNQIKQAVEAEAKTRGIAVV
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
TVDAKGDGPTQVNQIQDLLTQNIDALIYIPAGAAAATVPVKLAKAAGVPVINIDRNADGA
EEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCC
PGDTFLATDSVASAKSVCDYIIKQAGGAGKMVIIHGQKGTTPEVDRTKGCMESVKANSGV
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
KIVAEQWSNMWSQDEGFQIMQNMLQANPDVSIVFAQADGLALGAAQAIKVANPSQKIVVG
EEEHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEECCHHEEEECCCCCEEEEE
GFDGDTAALEALKNGVFDVTATQQTQKMGRMAVEMAVKIVGGEKVPAVQLSDATLTTKEN
CCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECHHH
VAGFIANHP
CCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]