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The map label for this gene is tig

Identifier: 159184864

GI number: 159184864

Start: 1650702

End: 1652180

Strand: Reverse

Name: tig

Synonym: Atu1664

Alternate gene names: 159184864

Gene position: 1652180-1650702 (Counterclockwise)

Preceding gene: 159184869

Following gene: 159184863

Centisome position: 58.14

GC content: 57.94

Gene sequence:

>1479_bases
ATGCAGGTTATCGAAACGCTCGCTGATGGGCTGAAGCGCGAACTCAAAATCGTTATCCCGGCTGCCGACATGAAGGCCCG
TCTGGATGAGCGCCTGGTTGACGCCAAGGACAAGGTTCGCATCAACGGTTTCCGTCCCGGCAAGGTGCCGATGGGCCATC
TGAAGAAGATGTACGGCAAGTCCATCATGGCCGACCTCGTCAACGAGCTGGTTCGTGAAAAGCCTTCCGAAATCCTTTCC
AGCCGCGGTGAGAAGTCTGCAACGCAGCCGGCCATCTCCATGACCGAAGACGAGCAGGAAGCCGAAAAGATCCTGTCGGC
CGAATCCGATTTCGAATTCACCGTTGCCTACGAAATCATTCCGGCCATCGAACTGAAGGCCAATGACGGCATCAAGGTTA
CCCGCGAAGTTGTTGAAGTCTCCGAAGACGAAATCAACGAGCAGATCCTCAAGATCGCCGAAAGCGCCCGTACCTACGAG
ACCAAGAAGGGCAAGGCCGCTGATGGCGACCGCGTCACTATGAACTATCTCGGCAAGGTTGACGGCGTTGCCTTCGATGG
CGGCACTGCTGAAGACGCTGAACTGGTTCTCGGTTCGGGCCGCTTCATCCCCGGCTTCGAAGACCAGCTCGTTGGCGTTA
AGGCCGGCGACGAAAAGACCATCACTGTGACCTTCCCGGCCGACTACCCGGCTGCGAACCTCGCAGGCAAGGACGCCACC
TTCGACGTCACCGTCAAGGAAGTTGCTGCCGCTGCTGCTGTCGAAATCAACGACGAACTGGCTGAAAAGCTCGGTCTCGA
ATCGGCTGAAAAGCTGAAGGAGATCGTCAAGGGCCAGATCGAAAGCCAGTTCGGCAACGTCACCCGCCAGAAGGTCAAGC
GTCAGATCCTCGACCAGCTGGACGAGATGTACAAGTTCGACACGCCGGCTGGCCTCGTCGACGCCGAGTTCGACAACATC
TGGCGCCAGATCAACACCGATCTCGCCCAGTCCGGCAAGACCTTCGCTGACGAAGACACGACCGAAGAAGAAGCCCGCGA
AGAATATCGCAAGCTTGCTGAACGCCGCGTCCGCCTCGGCCTCGTTCTTTCTGAAATCGGCGAAAAGGCCGGCGTCGAAG
TGACCGAAGAAGAAATGCAGCGCGCGCTGTTCCAGCAGCTGCAGCAGTTCCCGGGCCAGCAGAAGGAAATCCTCGATTTC
TTCCGCAACACCCCCGGCGCTTCCGCTTCGCTGCGCGCTCCGATCTTCGAAGAGAAGGTCATCGACAAGCTGCTCACCGA
AATCTCGGTAACGGACAAGACCGTTTCCAAGGAAGAGCTGCTGGCTGACGACGGCGAAGAAGAAACGGAAACCAAAAAGA
AGGCTCCGGCCAAGAAAAAGGCTGCTGCCAAGGCTGACGACGCCGCTGAAGGCGAAGAAGCCGCTCCGAAGAAGAAGGCT
CCGGCAAAGAAGAAGGCCACTGAGGCCGACGCCGAATAA

Upstream 100 bases:

>100_bases
TAAGGGAAGCCACGGCCGGGGAACGACGTTTCCGGCGCAGTGCTCGAAAGTAAACCAACGGCTGTCTGAGCACCGCCGCC
ATGATGTGAAGGTTAGGAAA

Downstream 100 bases:

>100_bases
TCTTCTTCAGTGCAAGCTGATTGAATTGAAGGGCCGTGGCGAAAGCTGCGGCCTTTTCGTTTGATTCAGGATGCGATGTG
TTCGGCCTTGAACCGGTTGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MQVIETLADGLKRELKIVIPAADMKARLDERLVDAKDKVRINGFRPGKVPMGHLKKMYGKSIMADLVNELVREKPSEILS
SRGEKSATQPAISMTEDEQEAEKILSAESDFEFTVAYEIIPAIELKANDGIKVTREVVEVSEDEINEQILKIAESARTYE
TKKGKAADGDRVTMNYLGKVDGVAFDGGTAEDAELVLGSGRFIPGFEDQLVGVKAGDEKTITVTFPADYPAANLAGKDAT
FDVTVKEVAAAAAVEINDELAEKLGLESAEKLKEIVKGQIESQFGNVTRQKVKRQILDQLDEMYKFDTPAGLVDAEFDNI
WRQINTDLAQSGKTFADEDTTEEEAREEYRKLAERRVRLGLVLSEIGEKAGVEVTEEEMQRALFQQLQQFPGQQKEILDF
FRNTPGASASLRAPIFEEKVIDKLLTEISVTDKTVSKEELLADDGEEETETKKKAPAKKKAAAKADDAAEGEEAAPKKKA
PAKKKATEADAE

Sequences:

>Translated_492_residues
MQVIETLADGLKRELKIVIPAADMKARLDERLVDAKDKVRINGFRPGKVPMGHLKKMYGKSIMADLVNELVREKPSEILS
SRGEKSATQPAISMTEDEQEAEKILSAESDFEFTVAYEIIPAIELKANDGIKVTREVVEVSEDEINEQILKIAESARTYE
TKKGKAADGDRVTMNYLGKVDGVAFDGGTAEDAELVLGSGRFIPGFEDQLVGVKAGDEKTITVTFPADYPAANLAGKDAT
FDVTVKEVAAAAAVEINDELAEKLGLESAEKLKEIVKGQIESQFGNVTRQKVKRQILDQLDEMYKFDTPAGLVDAEFDNI
WRQINTDLAQSGKTFADEDTTEEEAREEYRKLAERRVRLGLVLSEIGEKAGVEVTEEEMQRALFQQLQQFPGQQKEILDF
FRNTPGASASLRAPIFEEKVIDKLLTEISVTDKTVSKEELLADDGEEETETKKKAPAKKKAAAKADDAAEGEEAAPKKKA
PAKKKATEADAE
>Mature_492_residues
MQVIETLADGLKRELKIVIPAADMKARLDERLVDAKDKVRINGFRPGKVPMGHLKKMYGKSIMADLVNELVREKPSEILS
SRGEKSATQPAISMTEDEQEAEKILSAESDFEFTVAYEIIPAIELKANDGIKVTREVVEVSEDEINEQILKIAESARTYE
TKKGKAADGDRVTMNYLGKVDGVAFDGGTAEDAELVLGSGRFIPGFEDQLVGVKAGDEKTITVTFPADYPAANLAGKDAT
FDVTVKEVAAAAAVEINDELAEKLGLESAEKLKEIVKGQIESQFGNVTRQKVKRQILDQLDEMYKFDTPAGLVDAEFDNI
WRQINTDLAQSGKTFADEDTTEEEAREEYRKLAERRVRLGLVLSEIGEKAGVEVTEEEMQRALFQQLQQFPGQQKEILDF
FRNTPGASASLRAPIFEEKVIDKLLTEISVTDKTVSKEELLADDGEEETETKKKAPAKKKAAAKADDAAEGEEAAPKKKA
PAKKKATEADAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=444, Percent_Identity=31.7567567567568, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_AGRT5 (Q8UEU0)

Other databases:

- EMBL:   AE007869
- PIR:   AC2781
- PIR:   D97560
- RefSeq:   NP_354652.2
- ProteinModelPortal:   Q8UEU0
- STRING:   Q8UEU0
- GeneID:   1133702
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1664
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q8UEU0
- ProtClustDB:   PRK01490
- BioCyc:   ATUM176299-1:ATU1664-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 54150; Mature: 54150

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVIETLADGLKRELKIVIPAADMKARLDERLVDAKDKVRINGFRPGKVPMGHLKKMYGK
CCHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHH
SIMADLVNELVREKPSEILSSRGEKSATQPAISMTEDEQEAEKILSAESDFEFTVAYEII
HHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEE
PAIELKANDGIKVTREVVEVSEDEINEQILKIAESARTYETKKGKAADGDRVTMNYLGKV
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCC
DGVAFDGGTAEDAELVLGSGRFIPGFEDQLVGVKAGDEKTITVTFPADYPAANLAGKDAT
CCEEECCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCE
FDVTVKEVAAAAAVEINDELAEKLGLESAEKLKEIVKGQIESQFGNVTRQKVKRQILDQL
EEEEHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEMYKFDTPAGLVDAEFDNIWRQINTDLAQSGKTFADEDTTEEEAREEYRKLAERRVRLG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSEIGEKAGVEVTEEEMQRALFQQLQQFPGQQKEILDFFRNTPGASASLRAPIFEEKV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
IDKLLTEISVTDKTVSKEELLADDGEEETETKKKAPAKKKAAAKADDAAEGEEAAPKKKA
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCC
PAKKKATEADAE
CCHHHCCCCCCC
>Mature Secondary Structure
MQVIETLADGLKRELKIVIPAADMKARLDERLVDAKDKVRINGFRPGKVPMGHLKKMYGK
CCHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHH
SIMADLVNELVREKPSEILSSRGEKSATQPAISMTEDEQEAEKILSAESDFEFTVAYEII
HHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEE
PAIELKANDGIKVTREVVEVSEDEINEQILKIAESARTYETKKGKAADGDRVTMNYLGKV
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHCC
DGVAFDGGTAEDAELVLGSGRFIPGFEDQLVGVKAGDEKTITVTFPADYPAANLAGKDAT
CCEEECCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCE
FDVTVKEVAAAAAVEINDELAEKLGLESAEKLKEIVKGQIESQFGNVTRQKVKRQILDQL
EEEEHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEMYKFDTPAGLVDAEFDNIWRQINTDLAQSGKTFADEDTTEEEAREEYRKLAERRVRLG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLSEIGEKAGVEVTEEEMQRALFQQLQQFPGQQKEILDFFRNTPGASASLRAPIFEEKV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
IDKLLTEISVTDKTVSKEELLADDGEEETETKKKAPAKKKAAAKADDAAEGEEAAPKKKA
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCC
PAKKKATEADAE
CCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194