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The map label for this gene is kdsA

Identifier: 159184755

GI number: 159184755

Start: 1420608

End: 1421453

Strand: Direct

Name: kdsA

Synonym: Atu1424

Alternate gene names: 159184755

Gene position: 1420608-1421453 (Clockwise)

Preceding gene: 15888750

Following gene: 15888752

Centisome position: 49.99

GC content: 59.57

Gene sequence:

>846_bases
ATGAGCGTTACGAACAATCTCAAAGTCACCGCCGGCGATGGTGCCAGCAAGGTCTCCTTCTGCAATACCGAGCGTTTTTC
GCTGATTGCCGGCCCCTGCCAGATGGAAAGCCGCGACCATGCCTTCATGATTGCCGGCGTGCTCAAGGAACTGTGCGATA
GTCTGGGCATCGGCCTCGTTTACAAATCCTCCTTCGACAAGGCCAACCGCACCTCGCTTTCCGGCAAGCGTGGCATCGGT
CTTGACAGCGCGATGGAGATTTTCGCTGATCTCAAGAAGGAATTCGGCTTTCCGGTTCTGAGCGATATTCACACCGAGGA
ACAATGCGCCATCGTGTCCGAAGTGGTGGATGTGCTGCAGATCCCGGCCTTCCTCAGCCGGCAGACCGATCTTCTCGTTG
CCGCCGCCAAAACCGGCCGCGTCATCAACGTCAAAAAAGGCCAGTTCCTGGCGCCCTGGGACATGAAGAACGTGCTGGCC
AAGCTGAATGAAAGCGGCAACCCGAATGTGCTGCTGTGCGAACGCGGCGCGTCGTTCGGTTACAATACGCTCGTCTCCGA
CATGCGTTCGCTACCGATCATGGCTTCACTCGGTGCGCCCGTCATTTTCGACGCCACCCATTCGGTGCAGCAGCCGGGCG
GGCAGGGCGGTTCCACCGGCGGCCAGCGCGAATTCGTCGAGACTTTGGCGCGGGCGGCGGTGGCCGTCGGCGTAGCCGGT
CTCTTCATCGAGACCCACGAGGACCCGGACAATGCGCCTTCCGATGGCCCCAACATGGTCCACCTGAAAGACATGCCGAA
ACTGTTGGAAAAGCTTCTGGCCTTCGACGCCATCACCAAAGCCTGA

Upstream 100 bases:

>100_bases
TACTTGCTGCTAATGGCGTCTCCAGCATGGAAGCGGGCGGCCGGCTGGTGGTGCCCGCAGCACCCGGTCAGGGCGTCGCC
TTCGCCTTCGAGGAAATATG

Downstream 100 bases:

>100_bases
CACAGTGCGTTCGGTTTGTGCCAATCGATTTTAATGGCGGGCGCTCCGTTTCTGCCGCCCGCCGTCATTGTAATTTCTTC
GTCATCGTCTAAGACAGGCG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLVYKSSFDKANRTSLSGKRGIG
LDSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQIPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLA
KLNESGNPNVLLCERGASFGYNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAG
LFIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA

Sequences:

>Translated_281_residues
MSVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLVYKSSFDKANRTSLSGKRGIG
LDSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQIPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLA
KLNESGNPNVLLCERGASFGYNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAG
LFIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA
>Mature_280_residues
SVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLVYKSSFDKANRTSLSGKRGIGL
DSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQIPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLAK
LNESGNPNVLLCERGASFGYNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAGL
FIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=274, Percent_Identity=46.7153284671533, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_AGRT5 (Q8UFH3)

Other databases:

- EMBL:   AE007869
- PIR:   AH2751
- PIR:   H97532
- RefSeq:   NP_354432.2
- ProteinModelPortal:   Q8UFH3
- SMR:   Q8UFH3
- STRING:   Q8UFH3
- GeneID:   1133462
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1424
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   RSLPIMA
- PhylomeDB:   Q8UFH3
- ProtClustDB:   PRK05198
- BioCyc:   ATUM176299-1:ATU1424-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29975; Mature: 29844

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLV
CCCCCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
YKSSFDKANRTSLSGKRGIGLDSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQ
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHH
IPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLAKLNESGNPNVLLCERGASFG
HHHHHCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHHHHCCCCCCCEEEECCCCCCC
YNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAG
HHHHHHHHHHCCCHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA
EEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLV
CCCCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEE
YKSSFDKANRTSLSGKRGIGLDSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQ
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHH
IPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLAKLNESGNPNVLLCERGASFG
HHHHHCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHHHHCCCCCCCEEEECCCCCCC
YNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAG
HHHHHHHHHHCCCHHHCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LFIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA
EEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194