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The map label for this gene is pyrB [H]

Identifier: 159184703

GI number: 159184703

Start: 1300286

End: 1301227

Strand: Reverse

Name: pyrB [H]

Synonym: Atu1308

Alternate gene names: 159184703

Gene position: 1301227-1300286 (Counterclockwise)

Preceding gene: 15888634

Following gene: 15888632

Centisome position: 45.79

GC content: 61.04

Gene sequence:

>942_bases
ATGGTCTTCTTCCCCCATCGCCATCTCCTCGGCATCAAGGGGCTTTCCCATCAGGATATCACCCTGCTTCTCGATAAAGC
CGACGAGGCGGTGAAAATCAGCCGTCAGCGCGAGAAAAAGACCTCGACGCTGCGAGGCCTGACGCAGATCAACCTGTTCT
TTGAAGCCTCGACGCGCACGCAATCCTCCTTCGAGCTGGCCGGAAAGCGCCTCGGCGCAGACGTGATGAATATGTCGGTC
GGCAATTCCTCGGTGAAGAAGGGCGAAACGCTGATCGATACCGCGATGACGCTGAACGCCATGCGCCCGGATGTGCTGGT
GGTTCGCCATTCGTCGGCAGGTGCCGCAGCGCTGCTGGCACAAAAGGTCGCCTGCTCCGTGGTCAATGCCGGGGACGGCC
AGCATGAGCACCCGACACAGGCGCTGCTGGATGCGCTGACCATCCGCCGCGCCAAGGGCGAGCTTTCGGGTATCACGGTG
GCGATCTGCGGTGACGTGCTGCATTCGCGCGTCGCCCGCTCCAATATCATTCTGCTCAACCAGATGGGTGCGCGCGTGCG
GGTCGTCGCCCCCGCCACGCTTCTTCCCTCCGGTATCCGCGACATGAGCGTGGAAGTCTTCAACGACATGAAGGAAGGGC
TGAAGAATGCCGATGTGGTGATGATGCTGCGCCTGCAGCGTGAGCGCATGTCCGGCTCCTTCGTGCCTTCGGTGCGCGAA
TATTTCCACTATTACGGTCTCGACGCCGAAAAGCTGAAAGCGGCGAAGGAAGATGCGCTGGTCATGCATCCCGGTCCGAT
GAACCGTGGCGTGGAAATCGCCTCCGAAGTGGCGGACGGTCCGCAGAGCGTCATCGAAAGCCAGGTGGAAATGGGGGTTG
CCGTGCGCATGGCCGTCATGGAAACCCTGCTTGTCTCGCAAAACCAGGGTGAGCGCGTATGA

Upstream 100 bases:

>100_bases
TCCTGAGGCGTGTTCACAGCGAATGGAGCGCACGGATCAGCATTCGGGCGTTCCGGGCATTTTCACGGGAAAATGCGGCG
CCCGTCAGGAGGCAAACCGC

Downstream 100 bases:

>100_bases
GTGCCGTTACCGTTCTCAAGAACCTCCGAATCGTCGACCCCTCCCGCAATCTGGATGAGACCGGCAGTATCGTCATCGGA
GCCGATGGCACCATTCTCGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MVFFPHRHLLGIKGLSHQDITLLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQSSFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVVRHSSAGAAALLAQKVACSVVNAGDGQHEHPTQALLDALTIRRAKGELSGITV
AICGDVLHSRVARSNIILLNQMGARVRVVAPATLLPSGIRDMSVEVFNDMKEGLKNADVVMMLRLQRERMSGSFVPSVRE
YFHYYGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIESQVEMGVAVRMAVMETLLVSQNQGERV

Sequences:

>Translated_313_residues
MVFFPHRHLLGIKGLSHQDITLLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQSSFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVVRHSSAGAAALLAQKVACSVVNAGDGQHEHPTQALLDALTIRRAKGELSGITV
AICGDVLHSRVARSNIILLNQMGARVRVVAPATLLPSGIRDMSVEVFNDMKEGLKNADVVMMLRLQRERMSGSFVPSVRE
YFHYYGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIESQVEMGVAVRMAVMETLLVSQNQGERV
>Mature_313_residues
MVFFPHRHLLGIKGLSHQDITLLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQSSFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVVRHSSAGAAALLAQKVACSVVNAGDGQHEHPTQALLDALTIRRAKGELSGITV
AICGDVLHSRVARSNIILLNQMGARVRVVAPATLLPSGIRDMSVEVFNDMKEGLKNADVVMMLRLQRERMSGSFVPSVRE
YFHYYGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIESQVEMGVAVRMAVMETLLVSQNQGERV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=37.012987012987, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=35.5987055016181, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=34.8909657320872, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=37.012987012987, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=33.8658146964856, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34056; Mature: 34056

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFFPHRHLLGIKGLSHQDITLLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRT
CEECCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
QSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDVLVVRHSSAGAAALLA
CHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QKVACSVVNAGDGQHEHPTQALLDALTIRRAKGELSGITVAICGDVLHSRVARSNIILLN
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCEEEEE
QMGARVRVVAPATLLPSGIRDMSVEVFNDMKEGLKNADVVMMLRLQRERMSGSFVPSVRE
CCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
YFHYYGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIESQVEMGVAVRMAVM
HHHHHCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ETLLVSQNQGERV
HHHHHCCCCCCCC
>Mature Secondary Structure
MVFFPHRHLLGIKGLSHQDITLLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRT
CEECCCCHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
QSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDVLVVRHSSAGAAALLA
CHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QKVACSVVNAGDGQHEHPTQALLDALTIRRAKGELSGITVAICGDVLHSRVARSNIILLN
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCEEEEE
QMGARVRVVAPATLLPSGIRDMSVEVFNDMKEGLKNADVVMMLRLQRERMSGSFVPSVRE
CCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
YFHYYGLDAEKLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIESQVEMGVAVRMAVM
HHHHHCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ETLLVSQNQGERV
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA