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The map label for this gene is ycdT [H]

Identifier: 159184622

GI number: 159184622

Start: 1111295

End: 1112257

Strand: Reverse

Name: ycdT [H]

Synonym: Atu1119

Alternate gene names: 159184622

Gene position: 1112257-1111295 (Counterclockwise)

Preceding gene: 159184624

Following gene: 159184621

Centisome position: 39.14

GC content: 60.85

Gene sequence:

>963_bases
ATGAACATTGCGCCATTGCCGCGCAATTACGAACTGATCTATGAAATATTATCAGGACACAACCCGGCCATGGGCCGCGA
CATTCTCGCGCTCGGCAATGCGCCGCAGCAGCACGAGATCGATATTATTGGCCAGCGGCACAATCTGCCCGGCTTTTCCC
GGATCGAAGCCGAACAGATGGCGAGCGCGACCTTCGATACGCTGACGCAGATTTCCGCGCGGCTGGAGGCAAGCCTTAAA
CGCACCGAGACCTTCATGGACGCCCTCCAGCAGGATGAGGACAGCGAGCCGCCGGCAACGGAGCGTTTCCTGGAGCTTCT
GGGCGACATTCACGAGGAGCAGACGAGCCTTCGGCATTTCATTGCCATGGGGCTGGTGAAAATCCGTGAAGCCGAAAGAA
ACCGGGTGGAACTTCAGGCCGCCTCCCTGCGCGACAGCTTGACGGCGCTTCCCAACCGGGCCGCCTTTCTGGAAAAGCTC
GGCGCCCTTTTTGCAAAGGATCGGGCCGCGTCCGCCACCTCGCTGGTATTGCTCGACATCGATCATTTCCGGGAAATCAA
CGGCAAATATGGTTCTTCCGCCGGCAATAAGGCACTGCGACGGCTCGCCGCGCTGTTTCGCAAGACCATCAAGAAGGACG
ATTTCGTCGCTCGCATCGGTGGCGATGAATTCGCCTTTCTGTTTGCCGGGGTACCGCAGAATACGGCCGAGGCGATCGCC
GAGCGGCTGCGGCAAAGCGTAGAGGCCCTGCACTTCGCGACCAGTGGTGGCGATGGCGAGCATCTGACGGTTTCGATCGG
TGTCGCGGCGGTGGATGGCACCGCCACAGCCGCCGAATTTTTCGGCCATGCCGAACTCGCTTTGCTCTCGGCCCGCAGCG
GCACGCGCAACTGCGTTATCGGCTACTCGCGGGAGGTGGCGCAGCACAGCCGCAACAGCTATCTGGCACAGCTCGGCTCC
TGA

Upstream 100 bases:

>100_bases
GGTATTGGGGCTGTCAGTGACGACATCGGCGGGCGATAAGAAACGTGATCAGGCAAGAAGTTCTCTTGTCGTTACCAAAA
TCACCCAATTCATCGCCAAG

Downstream 100 bases:

>100_bases
AGCGGCAATCTCTCGCGGTGTGGCGCAGAGGCTCTTGCCTTGGCCAGACAGTTCGGCCAAAACGGCGCCATGATTACGAT
TACCGATCTTTCCGCCCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNIAPLPRNYELIYEILSGHNPAMGRDILALGNAPQQHEIDIIGQRHNLPGFSRIEAEQMASATFDTLTQISARLEASLK
RTETFMDALQQDEDSEPPATERFLELLGDIHEEQTSLRHFIAMGLVKIREAERNRVELQAASLRDSLTALPNRAAFLEKL
GALFAKDRAASATSLVLLDIDHFREINGKYGSSAGNKALRRLAALFRKTIKKDDFVARIGGDEFAFLFAGVPQNTAEAIA
ERLRQSVEALHFATSGGDGEHLTVSIGVAAVDGTATAAEFFGHAELALLSARSGTRNCVIGYSREVAQHSRNSYLAQLGS

Sequences:

>Translated_320_residues
MNIAPLPRNYELIYEILSGHNPAMGRDILALGNAPQQHEIDIIGQRHNLPGFSRIEAEQMASATFDTLTQISARLEASLK
RTETFMDALQQDEDSEPPATERFLELLGDIHEEQTSLRHFIAMGLVKIREAERNRVELQAASLRDSLTALPNRAAFLEKL
GALFAKDRAASATSLVLLDIDHFREINGKYGSSAGNKALRRLAALFRKTIKKDDFVARIGGDEFAFLFAGVPQNTAEAIA
ERLRQSVEALHFATSGGDGEHLTVSIGVAAVDGTATAAEFFGHAELALLSARSGTRNCVIGYSREVAQHSRNSYLAQLGS
>Mature_320_residues
MNIAPLPRNYELIYEILSGHNPAMGRDILALGNAPQQHEIDIIGQRHNLPGFSRIEAEQMASATFDTLTQISARLEASLK
RTETFMDALQQDEDSEPPATERFLELLGDIHEEQTSLRHFIAMGLVKIREAERNRVELQAASLRDSLTALPNRAAFLEKL
GALFAKDRAASATSLVLLDIDHFREINGKYGSSAGNKALRRLAALFRKTIKKDDFVARIGGDEFAFLFAGVPQNTAEAIA
ERLRQSVEALHFATSGGDGEHLTVSIGVAAVDGTATAAEFFGHAELALLSARSGTRNCVIGYSREVAQHSRNSYLAQLGS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=32.3353293413174, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=36, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=31.1827956989247, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=31.25, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=31.3609467455621, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=32.5443786982249, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082007, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=28.2722513089005, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787802, Length=126, Percent_Identity=34.9206349206349, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=28.9308176100629, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=156, Percent_Identity=31.4102564102564, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34957; Mature: 34957

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAPLPRNYELIYEILSGHNPAMGRDILALGNAPQQHEIDIIGQRHNLPGFSRIEAEQM
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHH
ASATFDTLTQISARLEASLKRTETFMDALQQDEDSEPPATERFLELLGDIHEEQTSLRHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAMGLVKIREAERNRVELQAASLRDSLTALPNRAAFLEKLGALFAKDRAASATSLVLLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEH
DHFREINGKYGSSAGNKALRRLAALFRKTIKKDDFVARIGGDEFAFLFAGVPQNTAEAIA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHH
ERLRQSVEALHFATSGGDGEHLTVSIGVAAVDGTATAAEFFGHAELALLSARSGTRNCVI
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHCCHHHHEEECCCCCCCEEE
GYSREVAQHSRNSYLAQLGS
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIAPLPRNYELIYEILSGHNPAMGRDILALGNAPQQHEIDIIGQRHNLPGFSRIEAEQM
CCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHH
ASATFDTLTQISARLEASLKRTETFMDALQQDEDSEPPATERFLELLGDIHEEQTSLRHF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAMGLVKIREAERNRVELQAASLRDSLTALPNRAAFLEKLGALFAKDRAASATSLVLLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEH
DHFREINGKYGSSAGNKALRRLAALFRKTIKKDDFVARIGGDEFAFLFAGVPQNTAEAIA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHH
ERLRQSVEALHFATSGGDGEHLTVSIGVAAVDGTATAAEFFGHAELALLSARSGTRNCVI
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHCCHHHHEEECCCCCCCEEE
GYSREVAQHSRNSYLAQLGS
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]