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The map label for this gene is rnhB

Identifier: 159184435

GI number: 159184435

Start: 719744

End: 720397

Strand: Reverse

Name: rnhB

Synonym: Atu0720

Alternate gene names: 159184435

Gene position: 720397-719744 (Counterclockwise)

Preceding gene: 159184436

Following gene: 159184426

Centisome position: 25.35

GC content: 64.98

Gene sequence:

>654_bases
ATGAAACGCACCGCCACACCCGATTCTCCTGCTCTTTTCGACCTTGTCGATACCGGCCCGGACTTCGCGTTCGAACTGGA
AGCCAAAAAGAAGGGCTTCTGGCCCGTGGCCGGCACGGATGAGGCAGGCCGGGGGCCACTTGCCGGCCCGGTGGTTGCGG
CGGCCGTCATTCTCGATCCGGATAATATTCCCAAAGGCATGGACGATTCCAAGAAGCTGACCAGGCAGAAACGGGAAAGC
CTTTTTGTGCAGATCATGGAAACCTCGATCGTGTCGGTCGCCTCCTCCGGCCCCGGCCTGATCGACAGCGTGAATATCCT
GCGTGCCAGCCTCGACGCCATGCGCCGCGCCGTGCTCGGCCTCGAAATCCCCCCCGCCCTCGTGCTGGCCGACGGCCGCG
ACAGGCCACCGGGCATCCCCTGCGAAGCCAAGGCCGTCATCAAGGGCGATTCCCGCTCCCTCTCCATCGCCGCCGCCTCG
ATCATCGCCAAGGTGACCCGCGACCGGATGATGGAACGGGCCGGCGTGGTGCACACCTCCTACGGCTTCGAAGGGCATGC
CGGTTACGGCACGCCGGCCCATCTGCGCGCCATCGAAAGCCATGGGCCTTGCGCGCTGCACCGGATGAGCTTTCGGCCGC
TGAAGCGGGATTGA

Upstream 100 bases:

>100_bases
GGCACGGTGAGCCACGCCCCTCGACCCCGGCTCACCGCGCCTGTCATCGCCCGCTTCCGGGGGCTTGCAGGAGATCGGGT
GTGTATGCGAGTTTCGCCGC

Downstream 100 bases:

>100_bases
GCGGCGAATACGGGACTTTTAATCTTCTCCGCTTTCAAAGCGGGTAGTTAAAAATGTTGCGTTTCTATTGAACCAATCTG
AGCGCGGCAATATCGCCGCT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKRTATPDSPALFDLVDTGPDFAFELEAKKKGFWPVAGTDEAGRGPLAGPVVAAAVILDPDNIPKGMDDSKKLTRQKRES
LFVQIMETSIVSVASSGPGLIDSVNILRASLDAMRRAVLGLEIPPALVLADGRDRPPGIPCEAKAVIKGDSRSLSIAAAS
IIAKVTRDRMMERAGVVHTSYGFEGHAGYGTPAHLRAIESHGPCALHRMSFRPLKRD

Sequences:

>Translated_217_residues
MKRTATPDSPALFDLVDTGPDFAFELEAKKKGFWPVAGTDEAGRGPLAGPVVAAAVILDPDNIPKGMDDSKKLTRQKRES
LFVQIMETSIVSVASSGPGLIDSVNILRASLDAMRRAVLGLEIPPALVLADGRDRPPGIPCEAKAVIKGDSRSLSIAAAS
IIAKVTRDRMMERAGVVHTSYGFEGHAGYGTPAHLRAIESHGPCALHRMSFRPLKRD
>Mature_217_residues
MKRTATPDSPALFDLVDTGPDFAFELEAKKKGFWPVAGTDEAGRGPLAGPVVAAAVILDPDNIPKGMDDSKKLTRQKRES
LFVQIMETSIVSVASSGPGLIDSVNILRASLDAMRRAVLGLEIPPALVLADGRDRPPGIPCEAKAVIKGDSRSLSIAAAS
IIAKVTRDRMMERAGVVHTSYGFEGHAGYGTPAHLRAIESHGPCALHRMSFRPLKRD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=51.0989010989011, Blast_Score=163, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_AGRT5 (Q8UHG2)

Other databases:

- EMBL:   AE007869
- PIR:   A97447
- PIR:   AB2665
- RefSeq:   NP_353745.2
- ProteinModelPortal:   Q8UHG2
- SMR:   Q8UHG2
- STRING:   Q8UHG2
- GeneID:   1132758
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0720
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q8UHG2
- ProtClustDB:   PRK00015
- BioCyc:   ATUM176299-1:ATU0720-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23134; Mature: 23134

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTATPDSPALFDLVDTGPDFAFELEAKKKGFWPVAGTDEAGRGPLAGPVVAAAVILDP
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECC
DNIPKGMDDSKKLTRQKRESLFVQIMETSIVSVASSGPGLIDSVNILRASLDAMRRAVLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
LEIPPALVLADGRDRPPGIPCEAKAVIKGDSRSLSIAAASIIAKVTRDRMMERAGVVHTS
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEC
YGFEGHAGYGTPAHLRAIESHGPCALHRMSFRPLKRD
CCCCCCCCCCCHHHHEEECCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKRTATPDSPALFDLVDTGPDFAFELEAKKKGFWPVAGTDEAGRGPLAGPVVAAAVILDP
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHEEEEEECC
DNIPKGMDDSKKLTRQKRESLFVQIMETSIVSVASSGPGLIDSVNILRASLDAMRRAVLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
LEIPPALVLADGRDRPPGIPCEAKAVIKGDSRSLSIAAASIIAKVTRDRMMERAGVVHTS
CCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEC
YGFEGHAGYGTPAHLRAIESHGPCALHRMSFRPLKRD
CCCCCCCCCCCHHHHEEECCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194