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The map label for this gene is pmbA [H]

Identifier: 159184421

GI number: 159184421

Start: 690689

End: 692035

Strand: Direct

Name: pmbA [H]

Synonym: Atu0693

Alternate gene names: 159184421

Gene position: 690689-692035 (Clockwise)

Preceding gene: 159184420

Following gene: 15888037

Centisome position: 24.31

GC content: 61.1

Gene sequence:

>1347_bases
ATGTCCTCAGAAATAGATTCTTCCAAACTTCTCGATCGCGCCAGCCAGCTTGTCGATCTCGCCCGTGCCGCCGGTGCGGA
TGAGGCGGATGCCGTTGTCGTGCGCTCCCGTTCGCAATCGGTCGGCGTGCGGCTTGGCAAGGTGGAAAGCACCGAATCCT
CCGAAAGCGATGATTTTTCCCTGCGTGTCTTCGTTGGCCGCCGGGTGGCAAGCGTTTCGGCCAATCCGGGTTTTGATCTG
AAGACCCTTGCCGAACGGGCCGTCGCCATGGCGAAGGTTTCCCCCGAAGACCCCTTTGCCTGCCTTGCGGACAAGCAACG
GCTGGCGACATCCTATGACGATCTCCAGCTTTTCGACGCGACGGAAGTCTCGGCGGATCAGCTTCGGGAAGCGGCACTTG
CCTCGGAAGAGGCGGCGCTGGCGGTCAAGGGCGTCAGCAATTCCTCCGGTGCCGGCGCATCGAGCGGCATGGGCGGGCTC
GTGCTCGTCACCTCACACGGTTTTTCCGGCAGCTATATGGGCAGCCGCTTCGGCCGCTCCGTCAGCGTCATCGCCGGCGA
AGGCACGAAGATGGAGCGCGACTACGATTACGATAGCCGCCTTTATTATGCTGATCTGGATACAGCCGAAGAGATCGGTC
GCCGCGCCGGGGAAAAAGTGGTGCGGCGCGTCGGCCCGCGTCAGGTCGATACCGGCAGCAATATCACTGTGGTGTTCGAT
CCCCGTATCGCCCGCGGTTTCGTCGGCGCCATTGCCGGTGCGATCAATGGTGCTTCGGTTGCCCGCAAGACCAGCTTCCT
GCGCGACAAGATGGGCCAGCAGATTCTGAAAAAAGGCCTTTATCTCACCGACGATCCCCAGATCGTGCGCGGCCCGTCCT
CCCGTCCCTTTGACGGCGAGGGTATCAGGGGTGAGAAGATGACGATGATCGAGGATGGTGTTTTGAAACACTGGTTCCTC
TCCACCTCGGCGGCGCGCGAGCTTGGCCTTGAAACCAATGGCCGCGGCGTGCGCGGCGGCACCTCGGTCTCGCCTGCCTC
CACCAACCTGGCGCTGGAGCCGGGCGATCTCTCACCGGAAGAATTGCTGGTGCAGATCGGAACCGGTTTTTACGTCACCG
AACTGATTGGTCACGGCGCCAACATGCTGACAGGTGAGTATAGCTGCGGTGCCAGCGGTTTCTGGATCGAGAACGGTGAA
AAATCCTTCCCCGTGTCCGAAGTCACCATCGCGTCCAACCTGAAGGACATGTTCATGCGGGTGACGCCTGCCAATGATAT
CGACCGCAAATATGGTGTCGCGGCACCGACGCTCGCCATCGAAGGCATGACGATCGCCGGCAAGTGA

Upstream 100 bases:

>100_bases
ATAGGGTGGCGATGGAGCCGTCTGAATGGAGAAAGCGGCGTTTCGCCCTTGATGCAGGGTTGCTGGCACAATAAATGGAA
CAGGAATGAAAGGCCAACTC

Downstream 100 bases:

>100_bases
TGCGGCCTGCGGGTTGTTTTCGCGGGCACTTTATGTGCAAGTGCGAAGAGAAAAACGGGCGCGCCGTTTGAGGCGCGCGG
AAAAGACGGCAAAATGAACG

Product: PmbA/TldD related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSSEIDSSKLLDRASQLVDLARAAGADEADAVVVRSRSQSVGVRLGKVESTESSESDDFSLRVFVGRRVASVSANPGFDL
KTLAERAVAMAKVSPEDPFACLADKQRLATSYDDLQLFDATEVSADQLREAALASEEAALAVKGVSNSSGAGASSGMGGL
VLVTSHGFSGSYMGSRFGRSVSVIAGEGTKMERDYDYDSRLYYADLDTAEEIGRRAGEKVVRRVGPRQVDTGSNITVVFD
PRIARGFVGAIAGAINGASVARKTSFLRDKMGQQILKKGLYLTDDPQIVRGPSSRPFDGEGIRGEKMTMIEDGVLKHWFL
STSAARELGLETNGRGVRGGTSVSPASTNLALEPGDLSPEELLVQIGTGFYVTELIGHGANMLTGEYSCGASGFWIENGE
KSFPVSEVTIASNLKDMFMRVTPANDIDRKYGVAAPTLAIEGMTIAGK

Sequences:

>Translated_448_residues
MSSEIDSSKLLDRASQLVDLARAAGADEADAVVVRSRSQSVGVRLGKVESTESSESDDFSLRVFVGRRVASVSANPGFDL
KTLAERAVAMAKVSPEDPFACLADKQRLATSYDDLQLFDATEVSADQLREAALASEEAALAVKGVSNSSGAGASSGMGGL
VLVTSHGFSGSYMGSRFGRSVSVIAGEGTKMERDYDYDSRLYYADLDTAEEIGRRAGEKVVRRVGPRQVDTGSNITVVFD
PRIARGFVGAIAGAINGASVARKTSFLRDKMGQQILKKGLYLTDDPQIVRGPSSRPFDGEGIRGEKMTMIEDGVLKHWFL
STSAARELGLETNGRGVRGGTSVSPASTNLALEPGDLSPEELLVQIGTGFYVTELIGHGANMLTGEYSCGASGFWIENGE
KSFPVSEVTIASNLKDMFMRVTPANDIDRKYGVAAPTLAIEGMTIAGK
>Mature_447_residues
SSEIDSSKLLDRASQLVDLARAAGADEADAVVVRSRSQSVGVRLGKVESTESSESDDFSLRVFVGRRVASVSANPGFDLK
TLAERAVAMAKVSPEDPFACLADKQRLATSYDDLQLFDATEVSADQLREAALASEEAALAVKGVSNSSGAGASSGMGGLV
LVTSHGFSGSYMGSRFGRSVSVIAGEGTKMERDYDYDSRLYYADLDTAEEIGRRAGEKVVRRVGPRQVDTGSNITVVFDP
RIARGFVGAIAGAINGASVARKTSFLRDKMGQQILKKGLYLTDDPQIVRGPSSRPFDGEGIRGEKMTMIEDGVLKHWFLS
TSAARELGLETNGRGVRGGTSVSPASTNLALEPGDLSPEELLVQIGTGFYVTELIGHGANMLTGEYSCGASGFWIENGEK
SFPVSEVTIASNLKDMFMRVTPANDIDRKYGVAAPTLAIEGMTIAGK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=446, Percent_Identity=38.3408071748879, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1789640, Length=216, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47486; Mature: 47355

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEIDSSKLLDRASQLVDLARAAGADEADAVVVRSRSQSVGVRLGKVESTESSESDDFS
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEE
LRVFVGRRVASVSANPGFDLKTLAERAVAMAKVSPEDPFACLADKQRLATSYDDLQLFDA
EEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHCCCCCEEEECC
TEVSADQLREAALASEEAALAVKGVSNSSGAGASSGMGGLVLVTSHGFSGSYMGSRFGRS
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCE
VSVIAGEGTKMERDYDYDSRLYYADLDTAEEIGRRAGEKVVRRVGPRQVDTGSNITVVFD
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEC
PRIARGFVGAIAGAINGASVARKTSFLRDKMGQQILKKGLYLTDDPQIVRGPSSRPFDGE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCC
GIRGEKMTMIEDGVLKHWFLSTSAARELGLETNGRGVRGGTSVSPASTNLALEPGDLSPE
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHH
ELLVQIGTGFYVTELIGHGANMLTGEYSCGASGFWIENGEKSFPVSEVTIASNLKDMFMR
HHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEE
VTPANDIDRKYGVAAPTLAIEGMTIAGK
ECCCCCCHHHCCCCCCEEEEECEEECCC
>Mature Secondary Structure 
SSEIDSSKLLDRASQLVDLARAAGADEADAVVVRSRSQSVGVRLGKVESTESSESDDFS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCEE
LRVFVGRRVASVSANPGFDLKTLAERAVAMAKVSPEDPFACLADKQRLATSYDDLQLFDA
EEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHCCCCCEEEECC
TEVSADQLREAALASEEAALAVKGVSNSSGAGASSGMGGLVLVTSHGFSGSYMGSRFGRS
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCE
VSVIAGEGTKMERDYDYDSRLYYADLDTAEEIGRRAGEKVVRRVGPRQVDTGSNITVVFD
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEC
PRIARGFVGAIAGAINGASVARKTSFLRDKMGQQILKKGLYLTDDPQIVRGPSSRPFDGE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCC
GIRGEKMTMIEDGVLKHWFLSTSAARELGLETNGRGVRGGTSVSPASTNLALEPGDLSPE
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHH
ELLVQIGTGFYVTELIGHGANMLTGEYSCGASGFWIENGEKSFPVSEVTIASNLKDMFMR
HHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEE
VTPANDIDRKYGVAAPTLAIEGMTIAGK
ECCCCCCHHHCCCCCCEEEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]