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The map label for this gene is cysL [H]

Identifier: 159184417

GI number: 159184417

Start: 668543

End: 669460

Strand: Direct

Name: cysL [H]

Synonym: Atu0672

Alternate gene names: 159184417

Gene position: 668543-669460 (Clockwise)

Preceding gene: 159185418

Following gene: 15888019

Centisome position: 23.53

GC content: 62.64

Gene sequence:

>918_bases
ATGACCTTCGAACAACTCCGTATTTTCATCGCCGTCGCCGAACGCGAACATCTGACCCGCGCCGCCGAAGCCATCGGCCT
CACGGCTTCCGCCGTCAGTTCCGCCATCAAGAATCTCGAGGCCTTTTATAATGTCGAGCTGTTCCACCGCGTCGGCCGCA
ACATCGAGTTGACCGAAAGCGGCCGGGCCTTTCTGGGCGAGGCCAAGGCGACGCTGGCGCGTGTGCGCAATGCCGAATTG
ATTCTCTCTGAGATGGGCGGCCTGACGCGCGGCGAAATTACCGTGTGCGCCAGCCAGACCATTGCAAGCTACTGGCTGCC
GCCGATCCTGATGCAGTTCAAGAAACTCTATCCCGGCGTGACGGTCCGGCTGGATATCGGCAATACCAAGACGGTAACGC
AGGCGGTGCTGGATGGTCTGGCGGAGGTTGGTTTCATCGAAGGCAAGATTGACGAGCCGGCATTGATGGTGCAGCCGGTC
GTGTCAGACAAGCTTCTGGTCGTCACCGGTTCGGCCCATCCTTTCGCTGACGGCCGTTATCTGACCGCGCTCGATATGCT
TTACGGCACATCCTGGGTTCTGCGCGAACAGGGCTCCGGCACGCGTTCCGCCTTCGAAGCAGCGGTGCGGCAGATGGGGG
TGGATCCGGCTGAGCTGTCCGTCATGCTCGAATTGCCATCCAATGAGGCGGTGGTGATGGCGGCCCGCTCCGGTGGCGCT
GCCACCGCCGTCTCCGCTTCCGTTGCGTCGCTTTTTCTGCGGCAGGGCCTGCTGGTCCGCGCCGGCATCGATCTGCCGGC
ACGCAATTTTGCTCTGTTGCGCCACAAGGAGCGCCATACCAGCCGCGCGGCGATGGAACTGGAGCGCCTCAGCCGGGCGG
CGTCGGAGGATTACAAGGCGGGTCTTGCGGGGTTGTAG

Upstream 100 bases:

>100_bases
GGGTGCGATGTTGTGCCATGGGCCGACAATGCCGTAGTCGCCTGTTTTATTCTATCAAATAATATGGCATATATCGTTCG
ATAAAATCGAATGAAACGCA

Downstream 100 bases:

>100_bases
GCCGAAGACGCAAGGCGCGTGACGATCACGTCATTTCCCGCGCAATGACGTGATGCACGATGCGATAATCCCGGCTTTCC
TCCCCGAAGGGCGCGAGCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTFEQLRIFIAVAEREHLTRAAEAIGLTASAVSSAIKNLEAFYNVELFHRVGRNIELTESGRAFLGEAKATLARVRNAEL
ILSEMGGLTRGEITVCASQTIASYWLPPILMQFKKLYPGVTVRLDIGNTKTVTQAVLDGLAEVGFIEGKIDEPALMVQPV
VSDKLLVVTGSAHPFADGRYLTALDMLYGTSWVLREQGSGTRSAFEAAVRQMGVDPAELSVMLELPSNEAVVMAARSGGA
ATAVSASVASLFLRQGLLVRAGIDLPARNFALLRHKERHTSRAAMELERLSRAASEDYKAGLAGL

Sequences:

>Translated_305_residues
MTFEQLRIFIAVAEREHLTRAAEAIGLTASAVSSAIKNLEAFYNVELFHRVGRNIELTESGRAFLGEAKATLARVRNAEL
ILSEMGGLTRGEITVCASQTIASYWLPPILMQFKKLYPGVTVRLDIGNTKTVTQAVLDGLAEVGFIEGKIDEPALMVQPV
VSDKLLVVTGSAHPFADGRYLTALDMLYGTSWVLREQGSGTRSAFEAAVRQMGVDPAELSVMLELPSNEAVVMAARSGGA
ATAVSASVASLFLRQGLLVRAGIDLPARNFALLRHKERHTSRAAMELERLSRAASEDYKAGLAGL
>Mature_304_residues
TFEQLRIFIAVAEREHLTRAAEAIGLTASAVSSAIKNLEAFYNVELFHRVGRNIELTESGRAFLGEAKATLARVRNAELI
LSEMGGLTRGEITVCASQTIASYWLPPILMQFKKLYPGVTVRLDIGNTKTVTQAVLDGLAEVGFIEGKIDEPALMVQPVV
SDKLLVVTGSAHPFADGRYLTALDMLYGTSWVLREQGSGTRSAFEAAVRQMGVDPAELSVMLELPSNEAVVMAARSGGAA
TAVSASVASLFLRQGLLVRAGIDLPARNFALLRHKERHTSRAAMELERLSRAASEDYKAGLAGL

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=32.280701754386, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI157672245, Length=250, Percent_Identity=27.6, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788748, Length=307, Percent_Identity=25.7328990228013, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693105, Length=220, Percent_Identity=25, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789173, Length=246, Percent_Identity=28.4552845528455, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=29.585798816568, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=179, Percent_Identity=29.0502793296089, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788296, Length=217, Percent_Identity=27.1889400921659, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788887, Length=187, Percent_Identity=29.9465240641711, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082132, Length=209, Percent_Identity=27.7511961722488, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=149, Percent_Identity=31.5436241610738, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=293, Percent_Identity=20.8191126279863, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32860; Mature: 32729

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEQLRIFIAVAEREHLTRAAEAIGLTASAVSSAIKNLEAFYNVELFHRVGRNIELTES
CCHHHEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GRAFLGEAKATLARVRNAELILSEMGGLTRGEITVCASQTIASYWLPPILMQFKKLYPGV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCE
TVRLDIGNTKTVTQAVLDGLAEVGFIEGKIDEPALMVQPVVSDKLLVVTGSAHPFADGRY
EEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCH
LTALDMLYGTSWVLREQGSGTRSAFEAAVRQMGVDPAELSVMLELPSNEAVVMAARSGGA
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCEEEEEECCCCC
ATAVSASVASLFLRQGLLVRAGIDLPARNFALLRHKERHTSRAAMELERLSRAASEDYKA
HHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLAGL
CCCCC
>Mature Secondary Structure 
TFEQLRIFIAVAEREHLTRAAEAIGLTASAVSSAIKNLEAFYNVELFHRVGRNIELTES
CHHHEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
GRAFLGEAKATLARVRNAELILSEMGGLTRGEITVCASQTIASYWLPPILMQFKKLYPGV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCE
TVRLDIGNTKTVTQAVLDGLAEVGFIEGKIDEPALMVQPVVSDKLLVVTGSAHPFADGRY
EEEEECCCCHHHHHHHHHHHHHCCEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCH
LTALDMLYGTSWVLREQGSGTRSAFEAAVRQMGVDPAELSVMLELPSNEAVVMAARSGGA
HHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCEEEEEECCCCC
ATAVSASVASLFLRQGLLVRAGIDLPARNFALLRHKERHTSRAAMELERLSRAASEDYKA
HHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLAGL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]