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The map label for this gene is folD

Identifier: 159184377

GI number: 159184377

Start: 572284

End: 573183

Strand: Direct

Name: folD

Synonym: Atu0589

Alternate gene names: 159184377

Gene position: 572284-573183 (Clockwise)

Preceding gene: 15887933

Following gene: 15887936

Centisome position: 20.14

GC content: 61.78

Gene sequence:

>900_bases
ATGGCAGTTGTGATTGACGGGAAGGCGAAGGCGGCTTCGGTAACGGAGGCGGTCCGCAAATCGGCAGAAGCGCTCGAGGC
GGAGAAGGGCGTGAAGCCCGGCCTCGCGGTTGTCATCGTCGGAAATGATCCGGCCAGCCACGCTTACGTGAACTCCAAGA
GCAAGATGGCGAAGCAATGCGGTTTCAACTCCATCCAGCACACGCTGCCGGAAGAAACGACGCAGGCGGCACTCTTGAAG
CTGGTCGGCGAACTGAACACCGATGCGTCCATCCACGGCATTCTGGTACAACTGCCCCTGCCGAAACATTTCAATTCGGA
TGAGATCATCCAGTCGATCCTGCCCGAGAAGGATGTCGATGGCCTCAGCGTCCTGAATGCCGGCAAGCTCGCTACCGGCG
ATCTCGCCACCGGTCTCATTTCCTGCACGCCAGCTGGCGCCATGCTGCTTGTGCGTGGCATTCACGGTGATGACCTTTCC
GGCCTCAACGCCGTTGTCATCGGCCGCTCGAACCTGTTCGGCAAGCCCATGGGCCAGCTCCTGCTGAACGCCAACGCCAC
GGTGACCATGGCGCATTCGCGCACCAAAGATCTTGTCACCGTCTGCAAGACGGCCGATATTCTCGTGGCTGCGGTCGGGC
GCGCGGCGATGGTGAAGGGCGACTGGGTAAAGCCCGGCGCAACCGTCATCGATGTCGGCATCAACCGCATTGCCGCACCG
GAAAAGGGCGAAGGCAAATCGAAACTGGTGGGCGATGTCGCCTTTGACGAGGCAAGCGCTGTTGCCGCCGCCATCACGCC
GGTGCCTGGCGGTGTCGGCCCGATGACGATCGCCATGCTGATGGCCAACACCGTCATCGCTGCCCATCGTGCGCTCGGCA
AGACCGCGCCGAAGTTCTGA

Upstream 100 bases:

>100_bases
AAGACTACCGCAAAACAGCCTGAAAACGCCGCATTTTAGCTTGCATTCCTGCCAAGAGGGCACCATACGGAGCCTACGTA
ACCCAGCAGGAGCAGGACGC

Downstream 100 bases:

>100_bases
TTGAATTGACCGGCCGGCTACGCCTTCGGAGCCGGCCCCGTCGAAGCGCGCACCACGAGTTCCGCCTTCCAGAGTTCCTG
ATGCGGTTCCGTCTGGTTGA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQCGFNSIQHTLPEETTQAALLK
LVGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVDGLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLS
GLNAVVIGRSNLFGKPMGQLLLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAP
EKGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF

Sequences:

>Translated_299_residues
MAVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQCGFNSIQHTLPEETTQAALLK
LVGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVDGLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLS
GLNAVVIGRSNLFGKPMGQLLLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAP
EKGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF
>Mature_298_residues
AVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQCGFNSIQHTLPEETTQAALLKL
VGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVDGLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLSG
LNAVVIGRSNLFGKPMGQLLLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAPE
KGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=299, Percent_Identity=47.1571906354515, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI222418558, Length=301, Percent_Identity=39.202657807309, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI94721354, Length=301, Percent_Identity=40.8637873754153, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI36796743, Length=228, Percent_Identity=26.7543859649123, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=287, Percent_Identity=47.7351916376307, Blast_Score=238, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=44.0677966101695, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=44.8275862068966, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=42.0529801324503, Blast_Score=228, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=23.0769230769231, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472483, Length=298, Percent_Identity=46.6442953020134, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI45551871, Length=298, Percent_Identity=46.6442953020134, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24645718, Length=298, Percent_Identity=46.6442953020134, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI17137370, Length=298, Percent_Identity=46.6442953020134, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI17136818, Length=303, Percent_Identity=45.8745874587459, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI17136816, Length=303, Percent_Identity=45.8745874587459, Blast_Score=227, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_AGRT5 (Q8UHU0)

Other databases:

- EMBL:   AE007869
- PIR:   AH2648
- PIR:   G97430
- RefSeq:   NP_353615.2
- HSSP:   P11586
- ProteinModelPortal:   Q8UHU0
- SMR:   Q8UHU0
- STRING:   Q8UHU0
- GeneID:   1132627
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0589
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   MIMIERI
- ProtClustDB:   PRK14188
- BioCyc:   ATUM176299-1:ATU0589-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30627; Mature: 30496

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQC
CEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCHHHHHHHH
GFNSIQHTLPEETTQAALLKLVGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVD
CCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLSGLNAVVIGRSNLFGKPMGQL
CCEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHH
LLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAP
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCEEEEECCHHCCCC
EKGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF
CCCCCCHHEEEHHHHCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVVIDGKAKAASVTEAVRKSAEALEAEKGVKPGLAVVIVGNDPASHAYVNSKSKMAKQC
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCHHHHHHHH
GFNSIQHTLPEETTQAALLKLVGELNTDASIHGILVQLPLPKHFNSDEIIQSILPEKDVD
CCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GLSVLNAGKLATGDLATGLISCTPAGAMLLVRGIHGDDLSGLNAVVIGRSNLFGKPMGQL
CCEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHH
LLNANATVTMAHSRTKDLVTVCKTADILVAAVGRAAMVKGDWVKPGATVIDVGINRIAAP
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCEEEEECCHHCCCC
EKGEGKSKLVGDVAFDEASAVAAAITPVPGGVGPMTIAMLMANTVIAAHRALGKTAPKF
CCCCCCHHEEEHHHHCHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194