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The map label for this gene is dnaN [H]

Identifier: 159184247

GI number: 159184247

Start: 293757

End: 294875

Strand: Reverse

Name: dnaN [H]

Synonym: Atu0301

Alternate gene names: 159184247

Gene position: 294875-293757 (Counterclockwise)

Preceding gene: 15887662

Following gene: 15887649

Centisome position: 10.38

GC content: 59.61

Gene sequence:

>1119_bases
ATGCGTATTACTCTTGAGCGGTCGAACCTTCTGAAATCGCTGAACCACGTCCACCGGGTCGTCGAGCGTCGCAACACGAT
CCCGATCCTGTCCAACGTTCTGCTGCGCGCCTCCGGCGCCAATCTGGACATGAAGGCGACCGACCTCGATCTGGAAATCA
CCGAAGCGACCCCGGCCATGGTGGAGCAGGCTGGCGCCACCACCGTACCGGCACACCTGCTTTACGAAATCGTGCGCAAG
CTGCCGGATGGTTCCGAAGTGCTTCTGGCGACCAACCCGGACGGCTCCTCCATGACCGTTGCGTCCGGCCGCTCGAAATT
CTCGCTGCAATGCCTGCCGGAAGCGGATTTCCCTGACCTCACCGCCGGCACCTTCAGCCACACCTTCAAACTGAAGGCGG
CCGATCTGAAGATGCTGATCGACCGGACGCAGTTTGCGATTTCGACCGAAGAGACGCGTTATTACCTGAACGGCATTTTC
TTCCACACCATCGAAAGCAATGGCGAGCTGAAACTGCGCGCCGTCGCCACCGACGGTCACCGCCTTGCGCGTGCTGACGT
CGATGCGCCCTCCGGCTCCGAAGGCATGCCGGGCATCATCATTCCGCGCAAGACCGTCGGTGAACTGCAGAAGCTGATGG
ACAATCCGGAACTGGAAGTCACAGTCGAAGTCTCGGATGCGAAGATCCGCCTGGCCATCGGTTCCGTCGTTCTGACCTCG
AAGCTGATCGACGGCACCTTTCCCGATTATCAGCGCGTCATCCCAACCGGCAACGACAAGGAAATGCGCGTCGATTGCCA
GACCTTCGCCCGGGCAGTGGACCGTGTTTCGACGATTTCTTCCGAGCGCGGCCGCGCCGTGAAGCTGGCGCTAACTGACG
GCCAGTTGACGCTGACCGTCAACAATCCCGACTCGGGAAGTGCTACCGAAGAAGTGGCCGTTGGCTACGACAATGATTCG
ATGGAAATCGGCTTCAATGCCAAATATCTCCTCGACATCACGTCGCAGCTCTCCGGCGAAGATGCGATTTTTCTGCTGGC
GGATGCGGGTTCGCCAACACTGGTTCGCGATACCGCCGGCGACGACGCACTCTATGTTCTGATGCCGATGCGCGTTTAA

Upstream 100 bases:

>100_bases
TGCCATTTTGCCCGTCCACATTGTCCGGCTTTTGCCGGTGAAGACTCTTCTTGCCGATCCGGCGGTGGCCGGTTCGCAAC
GGTTGAAAGCGGTAGCGTCC

Downstream 100 bases:

>100_bases
AACCGACCGTTTTCTTCAATTTTTCCAGAAACGCCGGTGGATCGCTTCATCGGCGTTTTTTGATTCGGCGAACAGGTGGC
TCTACCCGTAACTGAATTTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRASGANLDMKATDLDLEITEATPAMVEQAGATTVPAHLLYEIVRK
LPDGSEVLLATNPDGSSMTVASGRSKFSLQCLPEADFPDLTAGTFSHTFKLKAADLKMLIDRTQFAISTEETRYYLNGIF
FHTIESNGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLMDNPELEVTVEVSDAKIRLAIGSVVLTS
KLIDGTFPDYQRVIPTGNDKEMRVDCQTFARAVDRVSTISSERGRAVKLALTDGQLTLTVNNPDSGSATEEVAVGYDNDS
MEIGFNAKYLLDITSQLSGEDAIFLLADAGSPTLVRDTAGDDALYVLMPMRV

Sequences:

>Translated_372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRASGANLDMKATDLDLEITEATPAMVEQAGATTVPAHLLYEIVRK
LPDGSEVLLATNPDGSSMTVASGRSKFSLQCLPEADFPDLTAGTFSHTFKLKAADLKMLIDRTQFAISTEETRYYLNGIF
FHTIESNGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLMDNPELEVTVEVSDAKIRLAIGSVVLTS
KLIDGTFPDYQRVIPTGNDKEMRVDCQTFARAVDRVSTISSERGRAVKLALTDGQLTLTVNNPDSGSATEEVAVGYDNDS
MEIGFNAKYLLDITSQLSGEDAIFLLADAGSPTLVRDTAGDDALYVLMPMRV
>Mature_372_residues
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRASGANLDMKATDLDLEITEATPAMVEQAGATTVPAHLLYEIVRK
LPDGSEVLLATNPDGSSMTVASGRSKFSLQCLPEADFPDLTAGTFSHTFKLKAADLKMLIDRTQFAISTEETRYYLNGIF
FHTIESNGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLMDNPELEVTVEVSDAKIRLAIGSVVLTS
KLIDGTFPDYQRVIPTGNDKEMRVDCQTFARAVDRVSTISSERGRAVKLALTDGQLTLTVNNPDSGSATEEVAVGYDNDS
MEIGFNAKYLLDITSQLSGEDAIFLLADAGSPTLVRDTAGDDALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=41.7553191489362, Blast_Score=284, Evalue=6e-78,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40426; Mature: 40426

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRASGANLDMKATDLDLEITEATPAM
CEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCEEEEECCEEEEECCCHHH
VEQAGATTVPAHLLYEIVRKLPDGSEVLLATNPDGSSMTVASGRSKFSLQCLPEADFPDL
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFSHTFKLKAADLKMLIDRTQFAISTEETRYYLNGIFFHTIESNGELKLRAVATDGH
CCCCCCEEEEEEHHHHHHHHCCHHEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARADVDAPSGSEGMPGIIIPRKTVGELQKLMDNPELEVTVEVSDAKIRLAIGSVVLTS
EEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEECHHHHHH
KLIDGTFPDYQRVIPTGNDKEMRVDCQTFARAVDRVSTISSERGRAVKLALTDGQLTLTV
HHHCCCCCCHHEECCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEE
NNPDSGSATEEVAVGYDNDSMEIGFNAKYLLDITSQLSGEDAIFLLADAGSPTLVRDTAG
CCCCCCCCCCEEEEEECCCCEEECCCCEEEEEEHHHCCCCCEEEEEECCCCCEEEEECCC
DDALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MRITLERSNLLKSLNHVHRVVERRNTIPILSNVLLRASGANLDMKATDLDLEITEATPAM
CEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCEEEEECCEEEEECCCHHH
VEQAGATTVPAHLLYEIVRKLPDGSEVLLATNPDGSSMTVASGRSKFSLQCLPEADFPDL
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFSHTFKLKAADLKMLIDRTQFAISTEETRYYLNGIFFHTIESNGELKLRAVATDGH
CCCCCCEEEEEEHHHHHHHHCCHHEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARADVDAPSGSEGMPGIIIPRKTVGELQKLMDNPELEVTVEVSDAKIRLAIGSVVLTS
EEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEECHHHHHH
KLIDGTFPDYQRVIPTGNDKEMRVDCQTFARAVDRVSTISSERGRAVKLALTDGQLTLTV
HHHCCCCCCHHEECCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEE
NNPDSGSATEEVAVGYDNDSMEIGFNAKYLLDITSQLSGEDAIFLLADAGSPTLVRDTAG
CCCCCCCCCCEEEEEECCCCEEECCCCEEEEEEHHHCCCCCEEEEEECCCCCEEEEECCC
DDALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]