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The map label for this gene is pyrF

Identifier: 159184246

GI number: 159184246

Start: 291565

End: 292272

Strand: Reverse

Name: pyrF

Synonym: Atu0298

Alternate gene names: 159184246

Gene position: 292272-291565 (Counterclockwise)

Preceding gene: 15887648

Following gene: 15887646

Centisome position: 10.29

GC content: 62.57

Gene sequence:

>708_bases
ATGACCGCGCGCGAAAAACTCATCGTCGGGCTGGATGTTCCGACTGTGCAGCAGGCAGAAGACATCGTGTCGAAAATCGG
CGACGAGGTCCTGTTCTACAAGATCGGTTATCAGCTGGTATTCGCCGGGGGTCTTGAATTCGCGCGTGACCTCGTCCAGA
GCGGCAAGAAGGTCTTTCTCGACATGAAGCTGCTCGATATCGACAACACCGTCGCCTCGGGCGTCGAAAACATCGCCCGC
ATGGGCATGTCGATGCTGACGCTGCATGCCTATCCCAAAGCCATGCGTGCTGCCGTCAAGGCGGCGGAAGGCTCCGGCCT
CTGCCTGCTGGGCGTCACCGTGCTGACCTCCATGGATGACAGCGATCTGGTCGAGGCCGGTTACGCGTCCGACGCGCGTT
CGCTGGTGCTGCGCCGGGCTGAACAGGCACGCGAGGCCGGTATGGGCGGCATTGTCTGTTCGGCGGAAGAATCGACCGCT
GTGCGTGAAATTCTCGGCCCCGACCTTGCCGTGGTCACCCCCGGCATTCGCCCCGCCGGCGCCGATCTTGGCGATCAGAA
GCGGGTGATGACGCCCTATGACGCCATCAAGGCCGGTTCGAGCCATCTGGTTGTCGCCCGTCCCATCGTCAGGGCGGAAG
ACCCGAAAGCGGCGGCCCGCGCCATTCTGGACGATATGCTGCGCGCGAGTTTTCCGGCCAATCAGTAA

Upstream 100 bases:

>100_bases
GGTGGCAATCTTTTCCGCTTTTCCATCGCCGAATTTTCACTTGCAGCGGCCTCCCCCACATGCGACTGGACGGCAATGGA
AACATGGCAGGGATAAGACA

Downstream 100 bases:

>100_bases
CGACGACAGGAGGAGATGAAATGGCAAAGGGATATTGGATCGCCCAAGTCGATGTACGCGACAGCGAGCGTTACAAGGAC
TATGTCTCCACAGCCAAGCC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTAREKLIVGLDVPTVQQAEDIVSKIGDEVLFYKIGYQLVFAGGLEFARDLVQSGKKVFLDMKLLDIDNTVASGVENIAR
MGMSMLTLHAYPKAMRAAVKAAEGSGLCLLGVTVLTSMDDSDLVEAGYASDARSLVLRRAEQAREAGMGGIVCSAEESTA
VREILGPDLAVVTPGIRPAGADLGDQKRVMTPYDAIKAGSSHLVVARPIVRAEDPKAAARAILDDMLRASFPANQ

Sequences:

>Translated_235_residues
MTAREKLIVGLDVPTVQQAEDIVSKIGDEVLFYKIGYQLVFAGGLEFARDLVQSGKKVFLDMKLLDIDNTVASGVENIAR
MGMSMLTLHAYPKAMRAAVKAAEGSGLCLLGVTVLTSMDDSDLVEAGYASDARSLVLRRAEQAREAGMGGIVCSAEESTA
VREILGPDLAVVTPGIRPAGADLGDQKRVMTPYDAIKAGSSHLVVARPIVRAEDPKAAARAILDDMLRASFPANQ
>Mature_234_residues
TAREKLIVGLDVPTVQQAEDIVSKIGDEVLFYKIGYQLVFAGGLEFARDLVQSGKKVFLDMKLLDIDNTVASGVENIARM
GMSMLTLHAYPKAMRAAVKAAEGSGLCLLGVTVLTSMDDSDLVEAGYASDARSLVLRRAEQAREAGMGGIVCSAEESTAV
REILGPDLAVVTPGIRPAGADLGDQKRVMTPYDAIKAGSSHLVVARPIVRAEDPKAAARAILDDMLRASFPANQ

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=36.6812227074236, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_AGRT5 (P58638)

Other databases:

- EMBL:   AE007869
- PIR:   AB2613
- PIR:   H97394
- RefSeq:   NP_353328.2
- ProteinModelPortal:   P58638
- STRING:   P58638
- GeneID:   1132336
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0298
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   P58638
- ProtClustDB:   PRK00230
- BioCyc:   ATUM176299-1:ATU0298-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24948; Mature: 24817

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 63-63 BINDING 12-12 BINDING 34-34 BINDING 116-116 BINDING 177-177 BINDING 186-186 BINDING 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAREKLIVGLDVPTVQQAEDIVSKIGDEVLFYKIGYQLVFAGGLEFARDLVQSGKKVFL
CCCCCCEEEECCCCCHHHHHHHHHHHCCHHEEHHHCCHHEEECCHHHHHHHHHCCCEEEE
DMKLLDIDNTVASGVENIARMGMSMLTLHAYPKAMRAAVKAAEGSGLCLLGVTVLTSMDD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCC
SDLVEAGYASDARSLVLRRAEQAREAGMGGIVCSAEESTAVREILGPDLAVVTPGIRPAG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCEEEECCCCCCCC
ADLGDQKRVMTPYDAIKAGSSHLVVARPIVRAEDPKAAARAILDDMLRASFPANQ
CCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAREKLIVGLDVPTVQQAEDIVSKIGDEVLFYKIGYQLVFAGGLEFARDLVQSGKKVFL
CCCCCEEEECCCCCHHHHHHHHHHHCCHHEEHHHCCHHEEECCHHHHHHHHHCCCEEEE
DMKLLDIDNTVASGVENIARMGMSMLTLHAYPKAMRAAVKAAEGSGLCLLGVTVLTSMDD
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCCC
SDLVEAGYASDARSLVLRRAEQAREAGMGGIVCSAEESTAVREILGPDLAVVTPGIRPAG
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCEEEECCCCCCCC
ADLGDQKRVMTPYDAIKAGSSHLVVARPIVRAEDPKAAARAILDDMLRASFPANQ
CCCCCCCCCCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194